G protein-coupled receptors (GPCRs) are involved in a multitude of cellular signaling cascades and consequently are a prominent target for pharmaceutical drugs. In the past decades, a growing number of high-resolution structures of GPCRs has been solved, providing unprecedented insights into their mode of action. However, knowledge on the dynamical nature of GPCRs is equally important for a better functional understanding, which can be obtained by NMR spectroscopy. Here, we employed a...
[NMR paper] Probing the Conformational States of Neurotensin Receptor 1 Variants*by NMR Site-Directed Methyl Labeling.
Probing the Conformational States of Neurotensin Receptor 1 Variants*by NMR Site-Directed Methyl Labeling.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-7388-69-wiley-full-text.png Related Articles Probing the Conformational States of Neurotensin Receptor 1 Variants*by NMR Site-Directed Methyl Labeling.
Chembiochem. 2020 Sep 02;:
Authors: Goba I, Goricanec D, Schum D, Hillenbrand M, Plückthun A, Hagn F
Abstract
G-protein coupled receptors are key players mediating signal...
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[NMR paper] Impact of polyphenols on receptor-ligand interactions by NMR: the case of neurotensin (NT)-neurotensin receptor fragment (NTS1) complex.
Impact of polyphenols on receptor-ligand interactions by NMR: the case of neurotensin (NT)-neurotensin receptor fragment (NTS1) complex.
Impact of polyphenols on receptor-ligand interactions by NMR: the case of neurotensin (NT)-neurotensin receptor fragment (NTS1) complex.
J Biomol Struct Dyn. 2019 May 03;:1-12
Authors: Richard T, Petit E, Pouységu L, Monti JP, Bondon A, Sylla T, Mérillon JM, Quideau S, Da Costa G
Abstract
Ligand-receptor interactions can be implicated in many pathological events such as chronic...
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[NMR paper] Expression and Purification of a Functional E. coli 13CH3-Methionine-Labeled Thermostable Neurotensin Receptor 1 Variant for Solution NMR Studies.
Expression and Purification of a Functional E. coli 13CH3-Methionine-Labeled Thermostable Neurotensin Receptor 1 Variant for Solution NMR Studies.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles Expression and Purification of a Functional E. coli 13CH3-Methionine-Labeled Thermostable Neurotensin Receptor 1 Variant for Solution NMR Studies.
Methods Mol Biol. 2019;1947:31-55
Authors: Bumbak F, Bathgate RAD, Scott DJ, Gooley PR
...
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[NMR paper] Optimization and 13CH3 methionine labeling of a signaling competent neurotensin receptor 1 variant for NMR studies.
Optimization and 13CH3 methionine labeling of a signaling competent neurotensin receptor 1 variant for NMR studies.
Optimization and 13CH3 methionine labeling of a signaling competent neurotensin receptor 1 variant for NMR studies.
Biochim Biophys Acta. 2018 Mar 26;:
Authors: Bumbak F, Keen AC, Gunn NJ, Gooley PR, Bathgate RAD, Scott DJ
Abstract
Neurotensin is a 13-residue peptide that acts as a neuromodulator of classical neurotransmitters such as dopamine and glutamate in the mammalian central nervous system, mainly by...
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[NMR paper] Backbone (1)H, (15)N, (13)C NMR assignment of the 518-627 fragment of the androgen receptor encompassing N-terminal and DNA binding domains.
Backbone (1)H, (15)N, (13)C NMR assignment of the 518-627 fragment of the androgen receptor encompassing N-terminal and DNA binding domains.
Related Articles Backbone (1)H, (15)N, (13)C NMR assignment of the 518-627 fragment of the androgen receptor encompassing N-terminal and DNA binding domains.
Biomol NMR Assign. 2016 Jan 5;
Authors: Meyer S, Wang YH, Pérez-Escrivŕ P, Kieffer B
Abstract
Androgen receptor (AR) belongs to the nuclear receptor superfamily that are ligand dependent transcription factors. This protein binds to...
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01-07-2016 11:10 PM
[NMR paper] Elucidation by NMR solution of neurotensin in small unilamellar vesicle environment: molecular surveys for neurotensin receptor recognition.
Elucidation by NMR solution of neurotensin in small unilamellar vesicle environment: molecular surveys for neurotensin receptor recognition.
Related Articles Elucidation by NMR solution of neurotensin in small unilamellar vesicle environment: molecular surveys for neurotensin receptor recognition.
J Biomol Struct Dyn. 2013;31(8):809-17
Authors: Da Costa G, Bondon A, Delalande O, Mouret L, Monti JP
Abstract
Neurotensin (NT) is a tridecapeptide, hormone in the periphery and neurotransmitter in the brain. We used high-resolution nuclear...
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02-08-2014 05:45 PM
Optimization of amino acid type-specific (13)C and (15)N labeling for the backbone assignment of membrane proteins by solution- and solid-state NMR with the UPLABEL algorithm.
Optimization of amino acid type-specific (13)C and (15)N labeling for the backbone assignment of membrane proteins by solution- and solid-state NMR with the UPLABEL algorithm.
Optimization of amino acid type-specific (13)C and (15)N labeling for the backbone assignment of membrane proteins by solution- and solid-state NMR with the UPLABEL algorithm.
J Biomol NMR. 2010 Dec 18;
Authors: Hefke F, Bagaria A, Reckel S, Ullrich SJ, Dötsch V, Glaubitz C, Güntert P
We present a computational method for finding optimal labeling patterns for the backbone...
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Optimization of amino acid type-specific 13C and 15N labeling for the backbone assignment of membrane proteins by solution- and solid-state NMR with the UPLABEL algorithm
Optimization of amino acid type-specific 13C and 15N labeling for the backbone assignment of membrane proteins by solution- and solid-state NMR with the UPLABEL algorithm
Abstract We present a computational method for finding optimal labeling patterns for the backbone assignment of membrane proteins and other large proteins that cannot be assigned by conventional strategies. Following the approach of Kainosho and Tsuji (Biochemistry 21:6273â??6279 (1982)), types of amino acids are labeled with 13C or/and 15N such that cross peaks between 13CO(i â?? 1) and 15NH(i) result only for pairs...
NMR sample optimization and backbone assignment of a stabilized neurotensin receptor
Linear Mode
