NMR paper NMR probing of protein-protein interactions using reporter ligands and affinity tags.

		BioNMR > Educational resources > Journal club
[NMR paper] NMR probing of protein-protein interactions using reporter ligands and affinity tags.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

11-24-2010, 09:25 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,778

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

NMR probing of protein-protein interactions using reporter ligands and affinity tags.

NMR probing of protein-protein interactions using reporter ligands and affinity tags.

Related Articles NMR probing of protein-protein interactions using reporter ligands and affinity tags.

J Am Chem Soc. 2004 Feb 18;126(6):1636-7

Authors: Ludwiczek ML, Baminger B, Konrat R

A novel method is proposed for the detection and quantification of protein-protein interactions in solution. In this approach, one protein binding partner is tagged with a ligand binding domain, and protein-protein interaction is monitored via changes in the NMR relaxation of a reporter ligand which reversibly binds to the ligand binding domain. The particular benefit of the method is that only minute amounts of protein material and no isotope labeling are required. Its ease of implementation and the high-throughput capabilities make the method an attractive complement to existing proteomic methodology.

PMID: 14871086 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Probing the binding entropy of ligand-protein interactions by NMR. Probing the binding entropy of ligand-protein interactions by NMR. Related Articles Probing the binding entropy of ligand-protein interactions by NMR. Chembiochem. 2005 Sep;6(9):1585-91 Authors: Homans SW	nmrlearner	Journal club	0	12-01-2010 06:56 PM
[NMR paper] Competition STD NMR for the detection of high-affinity ligands and NMR-based screenin Competition STD NMR for the detection of high-affinity ligands and NMR-based screening. Related Articles Competition STD NMR for the detection of high-affinity ligands and NMR-based screening. Magn Reson Chem. 2004 Jun;42(6):485-9 Authors: Wang YS, Liu D, Wyss DF The reported competition STD NMR method combines saturation transfer difference (STD) NMR with competition binding experiments to allow the detection of high-affinity ligands that undergo slow chemical exchange on the NMR time-scale. With this technique, the presence of a competing...	nmrlearner	Journal club	0	11-24-2010 09:51 PM
[NMR paper] Probing the kinetic landscape of transient peptide-protein interactions by use of pep Probing the kinetic landscape of transient peptide-protein interactions by use of peptide (15)n NMR relaxation dispersion spectroscopy: binding of an antithrombin peptide to human prothrombin. Related Articles Probing the kinetic landscape of transient peptide-protein interactions by use of peptide (15)n NMR relaxation dispersion spectroscopy: binding of an antithrombin peptide to human prothrombin. J Am Chem Soc. 2003 Oct 15;125(41):12432-42 Authors: Tolkatchev D, Xu P, Ni F Protein-ligand interactions may lead to the formation of multiple...	nmrlearner	Journal club	0	11-24-2010 09:16 PM
[NMR paper] Probing residual interactions in unfolded protein states using NMR spin relaxation te Probing residual interactions in unfolded protein states using NMR spin relaxation techniques: an application to delta131delta. Related Articles Probing residual interactions in unfolded protein states using NMR spin relaxation techniques: an application to delta131delta. J Am Chem Soc. 2003 Oct 1;125(39):11988-92 Authors: Choy WY, Kay LE Residual interactions in delta131delta, a large disordered fragment of staphylococcal nuclease, have been probed at two different pHs using backbone (15)N and side-chain methyl (2)H NMR spin relaxation...	nmrlearner	Journal club	0	11-24-2010 09:16 PM
[NMR paper] Probing site-specific interactions in protein-DNA complexes using heteronuclear NMR s Probing site-specific interactions in protein-DNA complexes using heteronuclear NMR spectroscopy and molecular modeling: binding of Cro repressor to OR3. Related Articles Probing site-specific interactions in protein-DNA complexes using heteronuclear NMR spectroscopy and molecular modeling: binding of Cro repressor to OR3. J Biomol Struct Dyn. 1998 Aug;16(1):13-20 Authors: Edwards CA, Tung CS, Silks LA, Gatewood JM, Fee JA, Mariappan SV In this paper, a general method is developed to study site-specific interactions in DNA-protein complexes...	nmrlearner	Journal club	0	11-17-2010 11:15 PM
[NMR paper] Probing carbohydrate-protein interactions by high-resolution NMR spectroscopy. Probing carbohydrate-protein interactions by high-resolution NMR spectroscopy. Related Articles Probing carbohydrate-protein interactions by high-resolution NMR spectroscopy. Adv Exp Med Biol. 1998;435:29-38 Authors: Homans SW, Field RA, Milton MJ, Probert M, Richardson JM	nmrlearner	Journal club	0	11-17-2010 11:06 PM
Efficient protein production method for NMR using soluble protein tags with cold shoc Efficient protein production method for NMR using soluble protein tags with cold shock expression vector Abstract The E. coli protein expression system is one of the most useful methods employed for NMR sample preparation. However, the production of some recombinant proteins in E. coli is often hampered by difficulties such as low expression level and low solubility. To address these problems, a modified cold-shock expression system containing a glutathione S-transferase (GST) tag, the pCold-GST system, was investigated. The pCold-GST system successfully expressed 9 out of 10 proteins...	nmrlearner	Journal club	0	09-18-2010 04:53 AM
[NMR paper] Discovering high-affinity ligands for proteins: SAR by NMR. Discovering high-affinity ligands for proteins: SAR by NMR. Related Articles Discovering high-affinity ligands for proteins: SAR by NMR. Science. 1996 Nov 29;274(5292):1531-4 Authors: Shuker SB, Hajduk PJ, Meadows RP, Fesik SW A nuclear magnetic resonance (NMR)-based method is described in which small organic molecules that bind to proximal subsites of a protein are identified, optimized, and linked together to produce high-affinity ligands. The approach is called "SAR by NMR" because structure-activity relationships (SAR) are obtained from...	nmrlearner	Journal club	0	08-22-2010 02:20 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off