Defining protein oligomeric state and/or its changes in solution is of significant interest for many biophysical studies carried out in vitro, especially when the nature of the oligomeric state is crucial in the subsequent interpretation of experimental results and their biological relevance. Nuclear magnetic resonance (NMR) is a well-established methodology for the characterization of protein structure, dynamics, and interactions at the atomic level. As a spectroscopic method, NMR also provides...
[NMR paper] Role of rotational motion in diffusion NMR experiments on supramolecular assemblies: application to Sup35NM fibrils
Role of rotational motion in diffusion NMR experiments on supramolecular assemblies: application to Sup35NM fibrils
Pulsed-field gradient (PFG) NMR is an important tool for characterization of biomolecules and supramolecular assemblies. However, for micron-sized objects, such as amyloid fibrils, these experiments become difficult to interpret because in addition to translational diffusion they are also sensitive to rotational diffusion. We have constructed a mathematical theory describing the outcome of PFG NMR experiments on rod-like fibrils. To test its validity, we have studied the...
[NMR paper] Resolving biomolecular motion and interactions by R2 and R1? Relaxation Dispersion NMR.
Resolving biomolecular motion and interactions by R2 and R1? Relaxation Dispersion NMR.
Related Articles Resolving biomolecular motion and interactions by R2 and R1? Relaxation Dispersion NMR.
Methods. 2018 Apr 25;:
Authors: Walinda E, Morimoto D, Sugase K
Abstract
Among the tools of structural biology, NMR spectroscopy is unique in that it not only derives a static three-dimensional structure, but also provides an atomic-level description of the local fluctuations and global dynamics around this static structure. A battery of...
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[NMR paper] Measurement of (14)N quadrupole couplings in biomolecular solids using indirect-detection (14)N solid-state NMR with DNP.
Measurement of (14)N quadrupole couplings in biomolecular solids using indirect-detection (14)N solid-state NMR with DNP.
Related Articles Measurement of (14)N quadrupole couplings in biomolecular solids using indirect-detection (14)N solid-state NMR with DNP.
Chem Commun (Camb). 2017 Oct 26;:
Authors: Jarvis JA, Haies I, Lelli M, Rossini AJ, Kuprov I, Carravetta M, Williamson PTF
Abstract
The quadrupolar interaction experienced by the spin-1 (14)N nucleus is known to be extremely sensitive to local structure and dynamics....
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10-28-2017 08:03 AM
[NMR paper] Spin-state-selective methods in solution- and solid-state biomolecular (13)C NMR.
Spin-state-selective methods in solution- and solid-state biomolecular (13)C NMR.
Related Articles Spin-state-selective methods in solution- and solid-state biomolecular (13)C NMR.
Prog Nucl Magn Reson Spectrosc. 2015 Feb;84-85C:1-13
Authors: Felli IC, Pierattelli R
Abstract
Spin-state-selective methods to achieve homonuclear decoupling in the direct acquisition dimension of (13)C detected NMR experiments have been one of the key contributors to converting (13)C detected NMR experiments into really useful tools for studying...
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[NMR paper] Systematic solution to homo-oligomeric structures determined by NMR.
Systematic solution to homo-oligomeric structures determined by NMR.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-2250-98-WileyOnlineLibrary-Button_120x27px_FullText.gif Related Articles Systematic solution to homo-oligomeric structures determined by NMR.
Proteins. 2015 Jan 23;
Authors: Martin JW, Zhou P, Donald BR
Abstract
Protein structure determination by NMR has predominantly relied on simulated annealing-based conformational search for a converged fold using primarily...
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Spin-State-Selective Methods in Solution- and Solid-State Biomolecular 13C NMR
Spin-State-Selective Methods in Solution- and Solid-State Biomolecular 13C NMR
Publication date: Available online 1 November 2014
Source:Progress in Nuclear Magnetic Resonance Spectroscopy</br>
Author(s): Isabella C. Felli , Roberta Pierattelli</br>
Spin-state-selective methods to achieve homonuclear decoupling in the direct acquisition dimension of 13C detected NMR experiments have been one of the key contributors to converting 13C detected NMR experiments into really useful tools for studying biomolecules. We discuss here in detail the various methods that have...
NMR measurement of biomolecular translational and rotational motion for evaluating changes of protein oligomeric state in solution
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