Related ArticlesNMR of Macromolecular Assemblies and Machines at 1 GHz and Beyond: New Transformative Opportunities for Molecular Structural Biology.
Methods Mol Biol. 2018;1688:1-35
Authors: Quinn CM, Wang M, Polenova T
Abstract
As a result of profound gains in sensitivity and resolution afforded by ultrahigh magnetic fields, transformative applications in the fields of structural biology and materials science*are being realized. The development of dual low temperature superconducting (LTS)/high-temperature superconducting (HTS) magnets has enabled the achievement of magnetic fields above 1*GHz (23.5*T), which will open doors to*an unprecedented new range of applications. In this contribution, we discuss the promise of ultrahigh field magnetic resonance. We highlight several methodological developments pertinent at high-magnetic fields including measurement of (1)H-(1)H distances and (1)H chemical shift anisotropy in the solid state as well as studies of quadrupolar nuclei such as (17)O. Higher magnetic fields have advanced heteronuclear detection in solution NMR, valuable for applications including metabolomics and disordered proteins, as well as expanded use of proton detection in the solid state in conjunction with ultrafast magic angle spinning. We also present several recent applications to structural studies of the AP205 bacteriophage, the M2 channel from Influenza A, and biomaterials such as human bone. Gains in sensitivity and resolution from increased field strengths will enable advanced applications of NMR spectroscopy including in vivo studies of whole cells and intact virions.
[NMR paper] Structural biology of supramolecular assemblies by magic-angle spinning NMR spectroscopy.
Structural biology of supramolecular assemblies by magic-angle spinning NMR spectroscopy.
Related Articles Structural biology of supramolecular assemblies by magic-angle spinning NMR spectroscopy.
Q Rev Biophys. 2017 Jan;50:e1
Authors: Quinn CM, Polenova T
Abstract
In recent years, exciting developments in instrument technology and experimental methodology have advanced the field of magic-angle spinning (MAS) nuclear magnetic resonance (NMR) to new heights. Contemporary MAS NMR yields atomic-level insights into structure and...
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[NMR paper] Isotope-labeling strategies for solution NMR studies of macromolecular assemblies.
Isotope-labeling strategies for solution NMR studies of macromolecular assemblies.
Related Articles Isotope-labeling strategies for solution NMR studies of macromolecular assemblies.
Curr Opin Struct Biol. 2016 Jun 10;38:75-82
Authors: Zhang H, van Ingen H
Abstract
Proteins come together in macromolecular assemblies, recognizing and binding to each other through their structures, and operating on their substrates through their motions. Detailed characterization of these processes is particularly suited to NMR, a high-resolution...
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06-14-2016 08:23 PM
[NMR paper] Solid-state NMR: An emerging technique in structural biology of self-assemblies.
Solid-state NMR: An emerging technique in structural biology of self-assemblies.
Related Articles Solid-state NMR: An emerging technique in structural biology of self-assemblies.
Biophys Chem. 2015 Jul 16;
Authors: Habenstein B, Loquet A
Abstract
Protein self-assemblies are ubiquitous biological systems involved in many cellular processes, ranging from bacterial and viral infection to the propagation of neurodegenerative disorders. Studying the atomic three-dimensional structures of protein self-assemblies is a particularly...
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08-04-2015 03:00 PM
[NMR paper] Information-driven modeling of large macromolecular assemblies using NMR data.
Information-driven modeling of large macromolecular assemblies using NMR data.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Information-driven modeling of large macromolecular assemblies using NMR data.
J Magn Reson. 2014 Apr;241:103-14
Authors: van Ingen H, Bonvin AM
Abstract
Availability of high-resolution atomic structures is one of the prerequisites for a mechanistic understanding of biomolecular function. This atomic...
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11-18-2014 01:56 PM
[NMR paper] Structural Investigations of Molecular Machines by Solid-State NMR.
Structural Investigations of Molecular Machines by Solid-State NMR.
Related Articles Structural Investigations of Molecular Machines by Solid-State NMR.
Acc Chem Res. 2013 Mar 15;
Authors: Loquet A, Habenstein B, Lange A
Abstract
Essential biological processes such as cell motion, signaling,protein synthesis, and pathogen-host interactions rely on multifunctional molecular machines containing supramolecular assemblies, that is, noncovalently assembled protein subunits. Scientists would like to acquire a detailed atomic view of the complete...
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03-19-2013 01:22 PM
Translational Diffusion of Macromolecular Assemblies Measured Using Transverse-Relaxation-Optimized Pulsed Field Gradient NMR
Translational Diffusion of Macromolecular Assemblies Measured Using Transverse-Relaxation-Optimized Pulsed Field Gradient NMR
Reto Horst, Arthur L. Horwich and Kurt Wu?thrich
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja206531c/aop/images/medium/ja-2011-06531c_0003.gif
Journal of the American Chemical Society
DOI: 10.1021/ja206531c
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/NWK45WCbths
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Translational diffusion of macromolecular assemblies measured using transverse relaxation-optimized PFG-NMR.
Translational diffusion of macromolecular assemblies measured using transverse relaxation-optimized PFG-NMR.
Translational diffusion of macromolecular assemblies measured using transverse relaxation-optimized PFG-NMR.
J Am Chem Soc. 2011 Sep 16;
Authors: Horst R, Horwich AL, Wüthrich K
Abstract
ABSTRACT In structural biology, pulsed field gradient (PFG) NMR for characterization of size and hydrodynamic parameters of macromolecular solutes has the advantage over other techniques that the measurements can be recorded with identical solution...
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09-17-2011 08:21 PM
[NMR paper] Slow diffusion of macromolecular assemblies by a new pulsed field gradient NMR method
Slow diffusion of macromolecular assemblies by a new pulsed field gradient NMR method.
Related Articles Slow diffusion of macromolecular assemblies by a new pulsed field gradient NMR method.
J Am Chem Soc. 2003 Mar 5;125(9):2541-5
Authors: Ferrage F, Zoonens M, Warschawski DE, Popot JL, Bodenhausen G
The translational diffusion coefficient of an integral membrane protein/surfactant complex has been measured using a novel pulsed field gradient NMR method. In this new approach, the information about the localization of the molecules is...
NMR of Macromolecular Assemblies and Machines at 1 GHz and Beyond: New Transformative Opportunities for Molecular Structural Biology.
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