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Biochim Biophys Acta. 1998 Jun 11;1385(1):7-16
Authors: Aime S, Bettinelli M, Ferrari M, Razzano E, Terreno E
At physiological pH and in the presence of an excess of malonate ligand (MAL), the lanthanide ions (Ln=Eu(III), Gd(III) and Tb(III)) are under the form of [Ln(MAL)2(H2O)4]-. Upon addition of human serum albumin (HSA), formation of two different ternary adducts of stoichiometry HSA-Ln(MAL)x(H2O)q (x=2, q=2; x=2, q=4) is detected. On the basis of the reasonable assumption that the binding strength for the two sites on the protein are inversely proportional to the hydration state of the metal ion, stability constants of 4.0.103 M-1 and 3.5.102 M-1 have been evaluated for the system with q=2 and q=4, respectively. Whereas for the stronger binding site it is suggested that the protein provides two or three donor atoms to the coordination cage of the Ln(III) ion, in the case of the weaker binding site it is likely that it corresponds to a simple electrostatic interaction between the negatively charged [Ln(MAL)2(H2O)4]- and positively charged groups on the surface of the protein.
Mechanistic Studies of the Lithium Enolate of 4-Fluoroacetophenone: Rapid-Injection NMR Study of Enolate Formation, Dynamics, and Aldol Reactivity
Mechanistic Studies of the Lithium Enolate of 4-Fluoroacetophenone: Rapid-Injection NMR Study of Enolate Formation, Dynamics, and Aldol Reactivity
Kristopher J. Kolonko, Daniel J. Wherritt and Hans J. Reich
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja207218f/aop/images/medium/ja-2011-07218f_0005.gif
Journal of the American Chemical Society
DOI: 10.1021/ja207218f
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/CiY0RvJQuSc
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[NMR paper] 1H and 13C NMR characterization of hemiamidal isoniazid-NAD(H) adducts as possible in
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Chemistry. 2003 May 9;9(9):2034-8
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J Biol Chem. 2000 Oct 13;275(41):31908-13
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Reactivity of surface lysyl residues of proteins with a broad range of chemical agents has been proposed to be dependent on the catalytic microenvironment of the residue. We have investigated the acetylation of wild type...
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Biochim Biophys Acta. 2000 Mar 6;1474(1):23-30
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J Biomol NMR. 1994 May;4(3):411-32
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Chem Res Toxicol. 1992 Jan-Feb;5(1):34-41
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