Fluorine ( 19 F) incorporation into glycan binding proteins (lectins) has been achieved and exploited to monitor the binding to carbohydrate ligands by Nuclear Magnetic Resonance (NMR) Spectroscopy. Galectins are a family of lectins that bind carbohydrates, generally with weak affinities, through a combination of intermolecular interactions including a key CH-? stacking interaction involving a conserved Trp residue. Herein, two galectins, Galectin-3 (Gal3) and Galectin-8 (Gal8) with one and two...
[NMR paper] The recognition of glycans by protein receptors. Insights from NMR spectroscopy.
The recognition of glycans by protein receptors. Insights from NMR spectroscopy.
Related Articles The recognition of glycans by protein receptors. Insights from NMR spectroscopy.
Chem Commun (Camb). 2018 Apr 17;:
Authors: Ardá A, Jiménez-Barbero J
Abstract
Carbohydrates (glycans, saccharides, sugars) are everywhere. In fact, glycan-protein interactions are involved in many essential processes of life and disease. The understanding of the key structural details at the atomic and molecular level is of paramount importance to...
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[NMR paper] Protein-Carbohydrate Interactions Studied by NMR. from Molecular Recognition to Drug Design.
Protein-Carbohydrate Interactions Studied by NMR. from Molecular Recognition to Drug Design.
Related Articles Protein-Carbohydrate Interactions Studied by NMR. from Molecular Recognition to Drug Design.
Curr Protein Pept Sci. 2012 Dec 10;
Authors: Fernandez-Alonso MD, Diaz D, Berbis MA, Marcelo F, Jimenez-Barbero J
Abstract
Diseases that result from infection are, in general, a consequence of specific interactions between a pathogenic organism and the cells. The study of host-pathogen interactions has provided insights for the design of...
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[NMR paper] Molecular recognition of complex-type biantennary N-glycans by protein receptors: a 3D view on epitope selection by NMR.
Molecular recognition of complex-type biantennary N-glycans by protein receptors: a 3D view on epitope selection by NMR.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Molecular recognition of complex-type biantennary N-glycans by protein receptors: a 3D view on epitope selection by NMR.
J Am Chem Soc. 2013 Jan 29;
Authors: Arda A, Blasco P, Varon Silva D, Schubert V, André S, Bruix M, Cañada FJ, Gabius HJ, Unverzagt C, Jimenez-Barbero J
Abstract
The current surge in defining...
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[NMR paper] NMR investigations of protein-carbohydrate interactions: insights into the topology o
NMR investigations of protein-carbohydrate interactions: insights into the topology of the bound conformation of a lactose isomer and beta-galactosyl xyloses to mistletoe lectin and galectin-1.
Related Articles NMR investigations of protein-carbohydrate interactions: insights into the topology of the bound conformation of a lactose isomer and beta-galactosyl xyloses to mistletoe lectin and galectin-1.
Biochim Biophys Acta. 2001 Dec 19;1568(3):225-36
Authors: Alonso-Plaza JM, Canales MA, Jiménez M, Roldán JL, García-Herrero A, Iturrino L, Asensio JL,...
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[NMR paper] NMR investigations of protein-carbohydrate interactions binding studies and refined t
NMR investigations of protein-carbohydrate interactions binding studies and refined three-dimensional solution structure of the complex between the B domain of wheat germ agglutinin and N,N', N"-triacetylchitotriose.
Related Articles NMR investigations of protein-carbohydrate interactions binding studies and refined three-dimensional solution structure of the complex between the B domain of wheat germ agglutinin and N,N', N"-triacetylchitotriose.
Eur J Biochem. 2000 Jul;267(13):3965-78
Authors: Espinosa JF, Asensio JL, García JL, Laynez J, Bruix M, Wright...
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[NMR paper] NMR investigations of protein-carbohydrate interactions: studies on the relevance of
NMR investigations of protein-carbohydrate interactions: studies on the relevance of Trp/Tyr variations in lectin binding sites as deduced from titration microcalorimetry and NMR studies on hevein domains. Determination of the NMR structure of the complex between pseudohevein and N,N',N"-triacetylchitotriose.
Related Articles NMR investigations of protein-carbohydrate interactions: studies on the relevance of Trp/Tyr variations in lectin binding sites as deduced from titration microcalorimetry and NMR studies on hevein domains. Determination of the NMR structure of the complex...
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[NMR paper] NMR investigations of protein-carbohydrate interactions: refined three-dimensional st
NMR investigations of protein-carbohydrate interactions: refined three-dimensional structure of the complex between hevein and methyl beta-chitobioside.
Related Articles NMR investigations of protein-carbohydrate interactions: refined three-dimensional structure of the complex between hevein and methyl beta-chitobioside.
Glycobiology. 1998 Jun;8(6):569-77
Authors: Asensio JL, Cañada FJ, Bruix M, González C, Khiar N, Rodríguez-Romero A, Jiménez-Barbero J
The specific interaction of hevein with GlcNAc-containing oligosaccharides has been...
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11-17-2010 11:06 PM
[NMR paper] 19F NMR relaxation studies on 5-fluorotryptophan- and tetradeutero-5-fluorotryptophan
19F NMR relaxation studies on 5-fluorotryptophan- and tetradeutero-5-fluorotryptophan-labeled E. coli glucose/galactose receptor.
Related Articles 19F NMR relaxation studies on 5-fluorotryptophan- and tetradeutero-5-fluorotryptophan-labeled E. coli glucose/galactose receptor.
J Biomol NMR. 1996 Jun;7(4):261-72
Authors: Luck LA, Vance JE, O'Connell TM, London RE
19F NMR relaxation studies have been carried out on a fluorotryptophan-labeled E. coli periplasmic glucose/galactose receptor (GGR). The protein was derived from E. coli grown on a...
NMR Investigations of Protein-Carbohydrate Interactions: The Recognition of Glycans by Galectins Engineered with Fluorotryptophan Residues
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