Forkhead box O4 (FOXO4), a human transcription factor, recognizes target DNA through its forkhead domain (FHD) while maintaining comparable binding affinity to non-target DNA. The conserved region 3 (CR3), a transactivation domain, modulates DNA binding kinetics to FHD and contributes to target DNA selection, but the underlying mechanism of this selection remains elusive. Using paramagnetic relaxation enhancement analysis, we observed a minor state of CR3 close to FHD in the presence of...
[NMR paper] Solution structure of the Hop TPR2A domain and investigation of target druggability by NMR, biochemical and in silico approaches.
Solution structure of the Hop TPR2A domain and investigation of target druggability by NMR, biochemical and in silico approaches.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:--www.nature.com-images-npg_logo.gif http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.nature.com-images-lo_npg.gif http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:--www.ncbi.nlm.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.png Solution structure of the Hop TPR2A domain and investigation of target druggability by NMR, biochemical and in...
nmrlearner
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10-04-2020 05:33 AM
NMR in target driven drug discovery: why not?
NMR in target driven drug discovery: why not?
Abstract
No matter the source of compounds, drug discovery campaigns focused directly on the target are entirely dependent on a consistent stream of reliable data that reports on how a putative ligand interacts with the protein of interest. The data will derive from many sources including enzyme assays and many types of biophysical binding assays such as TR-FRET, SPR, thermophoresis and many others. Each method has its strengths and weaknesses, but none is as information rich and broadly applicable as NMR....
[ASAP] Protein Farnesyltransferase Catalyzes Unanticipated Farnesylation and Geranylgeranylation of Shortened Target Sequences
Protein Farnesyltransferase Catalyzes Unanticipated Farnesylation and Geranylgeranylation of Shortened Target Sequences
https://pubs.acs.org/na101/home/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.0c00081/20200310/images/medium/bi0c00081_0009.gif
Biochemistry
DOI: 10.1021/acs.biochem.0c00081
http://feeds.feedburner.com/~r/acs/bichaw/~4/_Z8PwCJXt2I
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nmrlearner
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03-16-2020 04:59 PM
[ASAP] Target Engagement and Binding Mode of an Antituberculosis Drug to Its Bacterial Target Deciphered in Whole Living Cells by NMR
Target Engagement and Binding Mode of an Antituberculosis Drug to Its Bacterial Target Deciphered in Whole Living Cells by NMR
https://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.8b00975/20190103/images/medium/bi-2018-00975t_0006.gif
Biochemistry
DOI: 10.1021/acs.biochem.8b00975
http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/bichaw/~4/J0ajkYKpz7s
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nmrlearner
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01-07-2019 05:49 AM
Discriminating Lipid– from Protein–CalciumBinding To Understand the Interaction between Recoverin and PhosphatidylglycerolModel Membranes
Discriminating Lipid– from Protein–CalciumBinding To Understand the Interaction between Recoverin and PhosphatidylglycerolModel Membranes
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.6b00408/20160610/images/medium/bi-2016-004082_0009.gif
Biochemistry
DOI: 10.1021/acs.biochem.6b00408
http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/bichaw/~4/AAOxpZR83vg
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nmrlearner
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06-11-2016 01:09 PM
HIV target: NMR revelations
HIV target: NMR revelations
http://www.spectroscopynow.com/common/images/thumbnails/14426a49cbd.jpgNMR spectroscopy has been used to home in on a vulnerable point in the HIV viral machinery, the envelope protein, gp41 membrane proximal external region. The research steals a march on other studies for which the protein is inaccessible, potentially leading the way to a vaccine or new targets for antiviral drugs against the disease.
Read the rest at Spectroscopynow.com
nmrlearner
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02-15-2014 07:22 AM
[NMR paper] Application of NMR SHAPES screening to an RNA target.
Application of NMR SHAPES screening to an RNA target.
Related Articles Application of NMR SHAPES screening to an RNA target.
J Am Chem Soc. 2003 Dec 24;125(51):15724-5
Authors: Johnson EC, Feher VA, Peng JW, Moore JM, Williamson JR
Several NMR screening techniques have been developed in recent years to aid in the identification of lead drug compounds. These NMR methods have traditionally been used for protein targets, and here we examine their applicability for an RNA target. We used the SHAPES compound library to test three different NMR...
NMR investigation of FOXO4-DNA interaction for discriminating target and non-target DNA sequences
Linear Mode
