?-Lactamases (EC 3.5.2.6) confer resistance against ?-lactam group-containing antibiotics in bacteria and higher eukaryotes, including humans. Pathogenic bacterial resistance against ?-lactam antibiotics is a primary concern for potential therapeutic developments and drug targets. Here, we report putative ?-lactamase activity, sulbactam binding (a ?-lactam analogue) in the low ?M affinity range, and site-specific interaction studies of a 14 kDa UV- and dark-inducible protein (abbreviated as...
Whole cell solid-state NMR study of Chlamydomonas reinhardtii microalgae
Whole cell solid-state NMR study of Chlamydomonas reinhardtii microalgae
Abstract
In vivo or whole-cell solid-state NMR is an emerging field which faces tremendous challenges. In most cases, cell biochemistry does not allow the labelling of specific molecules and an in vivo study is thus hindered by the inherent difficulty of identifying, among a formidable number of resonances, those arising from a given molecule. In this work we examined the possibility of studying, by solid-state NMR, the model organism Chlamydomonas reinhardtii fully and...
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[NMR paper] NMR backbone resonance assignment of New Delhi metallo-beta-lactamase.
NMR backbone resonance assignment of New Delhi metallo-beta-lactamase.
Related Articles NMR backbone resonance assignment of New Delhi metallo-beta-lactamase.
Biomol NMR Assign. 2017 Aug 14;:
Authors: Yao C, Wu Q, Xu G, Li C
Abstract
The emerging of the New Delhi metallo-beta-lactamase (NDM-1) has become one of the greatest threats to the clinical treatment. Although the structure of NDM-1 has been determined by X-ray crystallography, the molecular mechanism and process of catalysis reaction remain elusive. NMR spectroscopy plays...
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08-17-2017 01:01 PM
Dynamics of Lysine as a Heme Axial Ligand: NMR Analysisof the Chlamydomonas reinhardtii Hemoglobin THB1
Dynamics of Lysine as a Heme Axial Ligand: NMR Analysisof the Chlamydomonas reinhardtii Hemoglobin THB1
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.6b00926/20170118/images/medium/bi-2016-00926m_0015.gif
Biochemistry
DOI: 10.1021/acs.biochem.6b00926
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01-19-2017 08:56 AM
[NMR paper] The dynamics of lysine as a heme axial ligand: NMR analysis of the Chlamydomonas reinhardtii hemoglobin THB1.
The dynamics of lysine as a heme axial ligand: NMR analysis of the Chlamydomonas reinhardtii hemoglobin THB1.
Related Articles The dynamics of lysine as a heme axial ligand: NMR analysis of the Chlamydomonas reinhardtii hemoglobin THB1.
Biochemistry. 2016 Dec 29;:
Authors: Preimesberger MR, Majumdar A, Lecomte JT
Abstract
Nitrate metabolism in Chlamydomonas reinhardtii involves THB1, a monomeric hemoglobin thought to function as a nitric oxide dioxygenase (NOD). NOD activity requires dioxygen and nitric oxide binding followed by...
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[NMR paper] (1)H, (13)C and (15)N NMR assignments of Mg (2+) bound form of UV inducible transcript protein (UVI31+) from Chlamydomonas reinhardtii.
(1)H, (13)C and (15)N NMR assignments of Mg (2+) bound form of UV inducible transcript protein (UVI31+) from Chlamydomonas reinhardtii.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles (1)H, (13)C and (15)N NMR assignments of Mg (2+) bound form of UV inducible transcript protein (UVI31+) from Chlamydomonas reinhardtii.
Biomol NMR Assign. 2014 Mar 18;
Authors: Singh H, Verma D, Rao BJ, Chary KV
Abstract
Almost complete sequence specific...
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03-19-2014 10:43 PM
[NMR paper] (1)H, (13)C and (15)N NMR assignments of a mutant of UV inducible transcript (S55A-UVI31+) from Chlamydomonas reinhardtii.
(1)H, (13)C and (15)N NMR assignments of a mutant of UV inducible transcript (S55A-UVI31+) from Chlamydomonas reinhardtii.
(1)H, (13)C and (15)N NMR assignments of a mutant of UV inducible transcript (S55A-UVI31+) from Chlamydomonas reinhardtii.
Biomol NMR Assign. 2013 Aug 27;
Authors: Singh H, Rao BJ, Chary KV
Abstract
Almost complete sequence specific (1)H, (13)C and (15)N resonance assignments of a mutant of UV inducible transcript (S55A-UVI31+) from Chlamydomonas reinhardtii are reported, as a prelude to its structural and functional...
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08-28-2013 11:43 AM
[NMR paper] NMR characterization of the metallo-beta-lactamase from Bacteroides fragilis and its
NMR characterization of the metallo-beta-lactamase from Bacteroides fragilis and its interaction with a tight-binding inhibitor: role of an active-site loop.
Related Articles NMR characterization of the metallo-beta-lactamase from Bacteroides fragilis and its interaction with a tight-binding inhibitor: role of an active-site loop.
Biochemistry. 1999 Nov 2;38(44):14507-14
Authors: Scrofani SD, Chung J, Huntley JJ, Benkovic SJ, Wright PE, Dyson HJ
Understanding the structure and dynamics of the enzymes that mediate antibiotic resistance of...
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[NMR paper] The single mutation Trp35-->Ala in the 35-40 redox site of Chlamydomonas reinhardtii
The single mutation Trp35-->Ala in the 35-40 redox site of Chlamydomonas reinhardtii thioredoxin h affects its biochemical activity and the pH dependence of C36-C39 1H-13C NMR.
Related Articles The single mutation Trp35-->Ala in the 35-40 redox site of Chlamydomonas reinhardtii thioredoxin h affects its biochemical activity and the pH dependence of C36-C39 1H-13C NMR.
Eur J Biochem. 1998 Jul 1;255(1):185-95
Authors: Krimm I, Lemaire S, Ruelland E, Miginiac-Maslow M, Jaquot JP, Hirasawa M, Knaff DB, Lancelin JM
The role of the invariant Trp...
NMR insights into beta-Lactamase activity of UVI31+ Protein from Chlamydomonas reinhardtii
Linear Mode
