Directed evolution is a powerful tool for improving existing properties and imparting completely new functionalities to proteins^(1-4). Nonetheless, its potential in even small proteins is inherently limited by the astronomical number of possible amino acid sequences. Sampling the complete sequence space of a 100-residue protein would require testing of 20^(100) combinations, which is beyond any existing experimental approach. In practice, selective modification of relatively few residues is...
[NMR paper] NMR-Guided Directed Evolution of a Kemp Eliminase
NMR-Guided Directed Evolution of a Kemp Eliminase
Directed evolution has emerged as a powerful tool for improving protein properties and imparting completely new functionalities onto existing proteins. Several computational methods have been successful in predicting potential hotspots for directed evolution. However, they rely heavily on prior structural and/or functional information. To address these fundamental limitations, we focused on Nuclear Magnetic Resonance (NMR) as a tool to rapidly evolve enzymes without structural characterization....
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05-14-2022 03:47 AM
[ASAP] An Emerging Link between Robustness and Evolvability in Directed Protein Evolution
An Emerging Link between Robustness and Evolvability in Directed Protein Evolution
https://pubs.acs.org/na101/home/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.1c00142/20210419/images/medium/bi1c00142_0002.gif
Biochemistry
DOI: 10.1021/acs.biochem.1c00142
http://feeds.feedburner.com/~r/acs/bichaw/~4/I_6q6kcq08s
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[NMR paper] (19)F NMR-Guided Design of Glycomimetic Langerin Ligands.
(19)F NMR-Guided Design of Glycomimetic Langerin Ligands.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles (19)F NMR-Guided Design of Glycomimetic Langerin Ligands.
ACS Chem Biol. 2016 Sep 16;11(9):2407-13
Authors: Wamhoff EC, Hanske J, Schnirch L, Aretz J, Grube M, Varón Silva D, Rademacher C
Abstract
C-type lectin receptors (CLRs) play a pivotal role in pathogen defense and immune homeostasis. Langerin, a CLR predominantly expressed on Langerhans...
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06-27-2017 05:28 PM
[NMR paper] ?¹V NMR Crystallography of Vanadium Chloroperoxidase and Its Directed Evolution P395D/L241V/T343A Mutant: Protonation Environments of the Active Site.
?¹V NMR Crystallography of Vanadium Chloroperoxidase and Its Directed Evolution P395D/L241V/T343A Mutant: Protonation Environments of the Active Site.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles ?¹V NMR Crystallography of Vanadium Chloroperoxidase and Its Directed Evolution P395D/L241V/T343A Mutant: Protonation Environments of the Active Site.
J Am Chem Soc. 2015 Apr 29;137(16):5618-28
Authors: Gupta R, Hou G, Renirie R, Wever R, Polenova T
Abstract
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03-22-2016 01:46 PM
51V NMRCrystallography of Vanadium Chloroperoxidaseand Its Directed Evolution P395D/L241V/T343A Mutant: Protonation Environmentsof the Active Site
51V NMRCrystallography of Vanadium Chloroperoxidaseand Its Directed Evolution P395D/L241V/T343A Mutant: Protonation Environmentsof the Active Site
Rupal Gupta, Guangjin Hou, Rokus Renirie, Ron Wever and Tatyana Polenova
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.5b02635/20150420/images/medium/ja-2015-02635t_0011.gif
Journal of the American Chemical Society
DOI: 10.1021/jacs.5b02635
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/wtjqPzQxjn0
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04-21-2015 12:33 PM
[NMR paper] A NMR guided approach for CsrA-RNA crystallization.
A NMR guided approach for CsrA-RNA crystallization.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles A NMR guided approach for CsrA-RNA crystallization.
J Biomol NMR. 2013 Jan 29;
Authors: Koharudin LM, Boelens R, Kaptein R, Gronenborn AM
Abstract
Structure determination of protein-nucleic acid complexes remains a challenging task. Here we present a simple method for generating crystals of a CsrA-nucleic acid complex, guided entirely by results from...
NMR-guided directed evolution
Linear Mode
