We have used chemical shift perturbation (CSP) and saturation transfer difference (STD) NMR experiments to identify and characterize the binding of selected ligands to the receptor-binding domain (RBD) of the spike glycoprotein (S-protein) of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We also subjected full-length S-protein to STD NMR experiments, allowing correlations with RBD-based results. CSPs reveal the binding sites for heparin and fondaparinux, and affinities were...
[ASAP] NMR Experiments Provide Insights into Ligand-Binding to the SARS-CoV-2 Spike Protein Receptor-Binding Domain
NMR Experiments Provide Insights into Ligand-Binding to the SARS-CoV-2 Spike Protein Receptor-Binding Domain
Robert Creutznacher, Thorben Maass, Barbora Veselkova, George Ssebyatika, Thomas Krey, Martin Empting, Norbert Tautz, Martin Frank, Knut Ko?lbel, Charlotte Uetrecht, and Thomas Peters
https://pubs.acs.org/cms/10.1021/jacs.2c05603/asset/images/medium/ja2c05603_0004.gif
Journal of the American Chemical Society
DOI: 10.1021/jacs.2c05603
[NMR paper] Structural characterization of the N-linked glycans in the receptor binding domain of the SARS-CoV-2 spike protein and*their interactions with human lectins using NMR spectroscopy.
Structural characterization of the N-linked glycans in the receptor binding domain of the SARS-CoV-2 spike protein and*their interactions with human lectins using NMR spectroscopy.
Structural characterization of the N-linked glycans in the receptor binding domain of the SARS-CoV-2 spike protein and*their interactions with human lectins using NMR spectroscopy.
Angew Chem Int Ed Engl. 2020 Sep 11;:
Authors: Lenza MP, Oyenarte I, Diercks T, Quintana JI, Gimeno A, Bosch A, Coelho H, Diniz A, Peccati F, Delgado S, Valle M, Millet O, Abrescia NGA,...
[NMR paper] Solution NMR Studies of the Ligand-Binding Domain of an Orphan Nuclear Receptor Reveals a Dynamic Helix in the Ligand-Binding Pocket.
Solution NMR Studies of the Ligand-Binding Domain of an Orphan Nuclear Receptor Reveals a Dynamic Helix in the Ligand-Binding Pocket.
Solution NMR Studies of the Ligand-Binding Domain of an Orphan Nuclear Receptor Reveals a Dynamic Helix in the Ligand-Binding Pocket.
Biochemistry. 2018 Mar 16;:
Authors: Daffern N, Chen Z, Zhang Y, Pick L, Radhakrishnan I
Abstract
The ligand-binding domains (LBD) of the NR5A subfamily of nuclear receptors activate transcription via ligand-dependent and ligand-independent mechanisms. The...
NMR Experiments Provide Insights into Ligand-Binding to the SARS-CoV-2 Spike Protein Receptor-Binding Domain
Linear Mode
