A NMR experiment for simultaneous correlations of valine and leucine/isoleucine methyls with carbonyl chemical shifts in proteins.
J Biomol NMR. 2013 Dec 18;
Authors: Tugarinov V, Venditti V, Marius Clore G
Abstract
A methyl-detected 'out-and-back' NMR experiment for obtaining simultaneous correlations of methyl resonances of valine and isoleucine/leucine residues with backbone carbonyl chemical shifts, SIM-HMCM(CGCBCA)CO, is described. The developed pulse-scheme serves the purpose of convenience in recording a single data set for all Ile(?1), Leu(?) and Val(?) (ILV) methyl positions instead of acquiring two separate spectra selective for valine or leucine/isoleucine residues. The SIM-HMCM(CGCBCA)CO experiment can be used for ILV methyl assignments in moderately sized protein systems (up to ~100*kDa) where the backbone chemical shifts of (13)C(?), (13)C? and (13)CO are known from prior NMR studies and where some losses in sensitivity can be tolerated for the sake of an overall reduction in NMR acquisition time.
PMID: 24346684 [PubMed - as supplied by publisher]
[NMR paper] Probing Slow Chemical Exchange at Carbonyl Sites in Proteins by Chemical Exchange Saturation Transfer NMR Spectroscopy.
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Angew Chem Int Ed Engl. 2013 Feb 28;
Authors: Vallurupalli P, Kay LE
Abstract
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03-02-2013 11:45 AM
Simultaneous acquisition of 13Cαâ??15N and 1Hâ??15Nâ??15N sequential correlations in proteins: application of dual receivers in 3D HNN
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Abstract We describe here, adaptation of the HNN pulse sequence for multiple nuclei detection using two independent receivers by utilizing the detectable 13Cα transverse magnetization which was otherwise dephased out in the conventional HNN experiment. It enables acquisition of 2D 13Cαâ??15N sequential correlations along with the standard 3D 15Nâ??15Nâ??1H correlations, which provides directionality to sequential walk in HNN, on one hand, and enhances...
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12-31-2011 10:40 AM
[Question from NMRWiki Q&A forum] From what experiment only two bond correlations we can get in varian nmr?
From what experiment only two bond correlations we can get in varian nmr?
Hi., i have one compound i need only two bond correlation contours from quaternary carbon to proton. Is there any pulse sequence to find only two bond correlations ie, from quaternary carbon to protons with minimum amount of sample in "varian nmr".
Check if somebody has answered this question on NMRWiki QA forum
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12-28-2010 05:27 AM
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Authors: Cisnetti F, Loth K, Pelupessy P, Bodenhausen G
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[NMR paper] 1H(C) and 1H(N) total NOE correlations in a single 3D NMR experiment. 15N and 13C tim
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Simultaneous data acquisition in time-sharing (TS) multi-dimensional NMR experiments has been shown an effective means to reduce experimental...
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11-24-2010 09:16 PM
[NMR paper] (19)F NMR studies of the leucine-isoleucine-valine binding protein: evidence that a c
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J Biomol Struct Dyn. 2003 Oct;21(2):235-46
Authors: Salopek-Sondi B, Vaughan MD, Skeels MC, Honek JF, Luck LA
The leucine-isoleucine-valine binding protein (LIV) found in the periplasmic space of E. coli has been used as a structural model for a number of neuronal receptors. This...
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11-24-2010 09:16 PM
Measurement of carbonyl chemical shifts of excited protein states by relaxation dispersion NMR spectroscopy: comparison between uniformly and selectively 13C labeled samples
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Patrik Lundström, D. Flemming Hansen and Lewis E. Kay
Journal of Biomolecular NMR; 2008; 42(1); pp 35 - 47
Abstract: Carr–Purcell–Meiboom–Gill (CPMG) relaxation dispersion nuclear magnetic resonance (NMR) spectroscopy has emerged as a powerful method for quantifying chemical shifts of excited protein states. For many applications of the technique that involve the measurement of relaxation rates of carbon...
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09-21-2008 11:36 PM
Simultaneous detection of amide and methyl correlations using a time shared NMR experiment: application to binding epitope mapping
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Peter Würtz, Olli Aitio, Maarit Hellman and Perttu Permi
Journal of Biomolecular NMR; 2007; 39(2) pp 97 - 105
Abstract:
Simultaneous recording of different NMR parameters is an efficient way to reduce the overall experimental time and speed up structural studies of biological macromolecules. This can especially be beneficial in the case of fast NMR-based drug screening applications or for collecting NOE restraints, where prohibitively long data collection...
A NMR experiment for simultaneous correlations of valine and leucine/isoleucine methyls with carbonyl chemical shifts in proteins.
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