NMR paper NMR evidence for slow collective motions in cyanometmyoglobin.

		BioNMR > Educational resources > Journal club
[NMR paper] NMR evidence for slow collective motions in cyanometmyoglobin.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-22-2010, 03:31 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

NMR evidence for slow collective motions in cyanometmyoglobin.

NMR evidence for slow collective motions in cyanometmyoglobin.

Related Articles NMR evidence for slow collective motions in cyanometmyoglobin.

Nat Struct Biol. 1997 Apr;4(4):292-7

Authors: Tolman JR, Flanagan JM, Kennedy MA, Prestegard JH

Residual dipolar couplings observed in NMR spectra at very high magnetic fields have been measured to a high degree of accuracy for the paramagnetic protein cyanometmyoglobin. Deviations of these measurements from predictions based on available crystallographic and solution structures are largely systematic and well correlated within a given helix of this highly alpha-helical protein. These observations can be explained by invoking collective motion and small displacements of representative helices from their reported average positions in the solid state. Thus, the measurements appear to be capable of providing important insights into slower, collective protein motions, which are likely to be important for function, and which have been difficult to study using established experimental techniques.

PMID: 9095197 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Slow Motions in the HydrophobicCore of Chicken Villin Headpiece Subdomain and Their Contributionsto Configurational Entropy and Heat Capacity from Solid-State DeuteronNMR Measurements Slow Motions in the HydrophobicCore of Chicken Villin Headpiece Subdomain and Their Contributionsto Configurational Entropy and Heat Capacity from Solid-State DeuteronNMR Measurements http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/bi201515b/aop/images/medium/bi-2011-01515b_0011.gif Biochemistry DOI: 10.1021/bi201515b http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/bichaw/~4/XVIXMlZqlNE More...	nmrlearner	Journal club	0	11-19-2011 07:51 AM
Comparative analysis of essential collective dynamics and NMR-derived flexibility profiles in evolutionarily diverse prion proteins. Comparative analysis of essential collective dynamics and NMR-derived flexibility profiles in evolutionarily diverse prion proteins. Comparative analysis of essential collective dynamics and NMR-derived flexibility profiles in evolutionarily diverse prion proteins. Prion. 2011 Jul 1;5(3) Authors: Santo KP, Berjanskii M, Wishart DS, Stepanova M Abstract Collective motions on ns-?s time scales are known to have a major impact on protein folding, stability, binding and enzymatic efficiency. It is also believed that these motions may have an...	nmrlearner	Journal club	0	08-27-2011 04:53 PM
[NMR paper] Characterization of the internal motions of a chimeric protein by 13C NMR highlights Characterization of the internal motions of a chimeric protein by 13C NMR highlights the important dynamic consequences of the engineering on a millisecond time scale. Related Articles Characterization of the internal motions of a chimeric protein by 13C NMR highlights the important dynamic consequences of the engineering on a millisecond time scale. Eur J Biochem. 2000 Nov;267(22):6519-33 Authors: Wolff N, Guenneugues M, Gilquin B, Drakopoulou E, Vita C, Ménez A, Zinn-Justin S By transferring the central curaremimetic beta hairpin of the...	nmrlearner	Journal club	0	11-19-2010 08:29 PM
[NMR paper] Identification of slow motions in the reduced recombinant high-potential iron sulfur Identification of slow motions in the reduced recombinant high-potential iron sulfur protein I (HiPIP I) from Ectothiorhodospira halophila via 15N rotating-frame NMR relaxation measurements. Related Articles Identification of slow motions in the reduced recombinant high-potential iron sulfur protein I (HiPIP I) from Ectothiorhodospira halophila via 15N rotating-frame NMR relaxation measurements. J Biomol NMR. 1998 Aug;12(2):307-18 Authors: Banci L, Felli IC, Koulougliotis D Rotating-frame 15N relaxation rate (R1 rho) NMR experiments have been...	nmrlearner	Journal club	0	11-17-2010 11:15 PM
[NMR paper] NMR evidence for slow collective motions in cyanometmyoglobin. NMR evidence for slow collective motions in cyanometmyoglobin. Related Articles NMR evidence for slow collective motions in cyanometmyoglobin. Nat Struct Biol. 1997 Apr;4(4):292-7 Authors: Tolman JR, Flanagan JM, Kennedy MA, Prestegard JH Residual dipolar couplings observed in NMR spectra at very high magnetic fields have been measured to a high degree of accuracy for the paramagnetic protein cyanometmyoglobin. Deviations of these measurements from predictions based on available crystallographic and solution structures are largely systematic...	nmrlearner	Journal club	0	08-22-2010 03:03 PM
[NMR paper] Collective NMR relaxation model applied to protein dynamics. Collective NMR relaxation model applied to protein dynamics. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/-PMGifs-Toolbar-topub.gif Related Articles Collective NMR relaxation model applied to protein dynamics. Phys Rev Lett. 1994 Feb 7;72(6):940-943 Authors: BrÃ¼schweiler R, Case DA	nmrlearner	Journal club	0	08-22-2010 03:33 AM
[NMR paper] Collective NMR relaxation model applied to protein dynamics. Collective NMR relaxation model applied to protein dynamics. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/-PMGifs-Toolbar-topub.gif Related Articles Collective NMR relaxation model applied to protein dynamics. Phys Rev Lett. 1994 Feb 7;72(6):940-943 Authors: BrÃ¼schweiler R, Case DA	nmrlearner	Journal club	0	08-22-2010 03:33 AM
[NMR paper] Solution structural characteristics of cyanometmyoglobin: resonance assignment of hem Solution structural characteristics of cyanometmyoglobin: resonance assignment of heme cavity residues by two-dimensional NMR. Related Articles Solution structural characteristics of cyanometmyoglobin: resonance assignment of heme cavity residues by two-dimensional NMR. Biochemistry. 1990 Feb 13;29(6):1545-56 Authors: Emerson SD, La Mar G Steady-state nuclear Overhauser effects (NOE), two-dimensional (2D) nuclear Overhauser effect spectroscopy (NOESY), and 2D spin correlation spectroscopy (COSY) have been applied to the fully paramagnetic...	nmrlearner	Journal club	0	08-21-2010 10:48 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off