NMR paper NMR evidence for independent domain structures in zoocin A, an antibacterial exoenzym

		BioNMR > Educational resources > Journal club
[NMR paper] NMR evidence for independent domain structures in zoocin A, an antibacterial exoenzym

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

11-24-2010, 09:51 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

NMR evidence for independent domain structures in zoocin A, an antibacterial exoenzym

NMR evidence for independent domain structures in zoocin A, an antibacterial exoenzyme.

Related Articles NMR evidence for independent domain structures in zoocin A, an antibacterial exoenzyme.

Biochem Biophys Res Commun. 2004 Apr 30;317(2):527-30

Authors: Liang Q, Simmonds RS, Timkovich R

NMR was used to obtain spectroscopic evidence supporting a two domain model for zoocin A in which an N-terminal catalytic domain is linked by a threonine-proline rich linker to a target recognition domain responsible for recognizing the cell wall of bacteria susceptible to the bacteriolytic action of the enzyme. When cloned and separately expressed, each domain retains the folding found in the whole enzyme. Additionally, spectroscopy suggests that the target recognition domain has a conformation typical of a soluble globular protein, while the catalytic domain aggregates at low millimolar concentrations.

PMID: 15063789 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Revealing Protein Structures in Solid-Phase Peptide Synthesis by 13C Solid-State NMR: Evidence of Excessive Misfolding for Alzheimer’s ? Revealing Protein Structures in Solid-Phase Peptide Synthesis by 13C Solid-State NMR: Evidence of Excessive Misfolding for Alzheimer’s ? Songlin Wang and Yoshitaka Ishii http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja212190z/aop/images/medium/ja-2011-12190z_0002.gif Journal of the American Chemical Society DOI: 10.1021/ja212190z http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/jacsat/~4/6EE7uthrnLg	nmrlearner	Journal club	0	01-31-2012 08:34 PM
Evidence from solid-state NMR for nonhelical conformations in the transmembrane domain of the amyloid precursor protein. Evidence from solid-state NMR for nonhelical conformations in the transmembrane domain of the amyloid precursor protein. Evidence from solid-state NMR for nonhelical conformations in the transmembrane domain of the amyloid precursor protein. Biophys J. 2011 Feb 2;100(3):711-9 Authors: Lu JX, Yau WM, Tycko R The amyloid precursor protein (APP) is subject to proteolytic processing by ?-secretase within neuronal membranes, leading to Alzheimer's disease-associated ?-amyloid peptide production by cleavage near the midpoint of the*single...	nmrlearner	Journal club	0	02-02-2011 12:40 PM
[NMR paper] Channel-forming membrane permeabilization by an antibacterial protein, sapecin: deter Channel-forming membrane permeabilization by an antibacterial protein, sapecin: determination of membrane-buried and oligomerization surfaces by NMR. Related Articles Channel-forming membrane permeabilization by an antibacterial protein, sapecin: determination of membrane-buried and oligomerization surfaces by NMR. J Biol Chem. 2004 Feb 6;279(6):4981-7 Authors: Takeuchi K, Takahashi H, Sugai M, Iwai H, Kohno T, Sekimizu K, Natori S, Shimada I The action mechanism of sapecin, an antibacterial peptide with membrane permeabilization activity, was...	nmrlearner	Journal club	0	11-24-2010 09:16 PM
[NMR paper] 1H NMR evidence that Glu-38 interacts with the N-terminal functional domain in interl 1H NMR evidence that Glu-38 interacts with the N-terminal functional domain in interleukin-8. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles 1H NMR evidence that Glu-38 interacts with the N-terminal functional domain in interleukin-8. FEBS Lett. 1996 Dec 9;399(1-2):43-6 Authors: Rajarathnam K, Clark-Lewis I, Dewald B, Baggiolini M, Sykes BD In order to assess the importance of the buried Glu-38 observed in the structure of interleukin-8, an analog in which Glu-38 was...	nmrlearner	Journal club	0	08-22-2010 02:20 PM
[NMR paper] Comparison of the NMR and X-ray structures of the HIV-1 matrix protein: evidence for Comparison of the NMR and X-ray structures of the HIV-1 matrix protein: evidence for conformational changes during viral assembly. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Comparison of the NMR and X-ray structures of the HIV-1 matrix protein: evidence for conformational changes during viral assembly. Protein Sci. 1996...	nmrlearner	Journal club	0	08-22-2010 02:20 PM
[NMR paper] Catalytic activity of the SH2 domain of human pp60c-src; evidence from NMR, mass spec Catalytic activity of the SH2 domain of human pp60c-src; evidence from NMR, mass spectrometry, site-directed mutagenesis and kinetic studies for an inherent phosphatase activity. Related Articles Catalytic activity of the SH2 domain of human pp60c-src; evidence from NMR, mass spectrometry, site-directed mutagenesis and kinetic studies for an inherent phosphatase activity. Biochemistry. 1995 Nov 21;34(46):15351-8 Authors: Boerner RJ, Consler TG, Gampe RT, Weigl D, Willard DH, Davis DG, Edison AM, Loganzo F, Kassel DB, Xu RX During solution...	nmrlearner	Journal club	0	08-22-2010 03:50 AM
[NMR paper] Two crystal structures of the B1 immunoglobulin-binding domain of streptococcal prote Two crystal structures of the B1 immunoglobulin-binding domain of streptococcal protein G and comparison with NMR. Related Articles Two crystal structures of the B1 immunoglobulin-binding domain of streptococcal protein G and comparison with NMR. Biochemistry. 1994 Apr 19;33(15):4721-9 Authors: Gallagher T, Alexander P, Bryan P, Gilliland GL The structure of the 56-residue B1 immunoglobulin-binding domain from streptococcal protein G has been determined in two different crystal forms. The crystal structures were deduced by molecular...	nmrlearner	Journal club	0	08-22-2010 03:33 AM
[NMR paper] Two crystal structures of the B1 immunoglobulin-binding domain of streptococcal prote Two crystal structures of the B1 immunoglobulin-binding domain of streptococcal protein G and comparison with NMR. Related Articles Two crystal structures of the B1 immunoglobulin-binding domain of streptococcal protein G and comparison with NMR. Biochemistry. 1994 Apr 19;33(15):4721-9 Authors: Gallagher T, Alexander P, Bryan P, Gilliland GL The structure of the 56-residue B1 immunoglobulin-binding domain from streptococcal protein G has been determined in two different crystal forms. The crystal structures were deduced by molecular...	nmrlearner	Journal club	0	08-22-2010 03:33 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off