NMR paper NMR elucidation of reduced B-Z transition activity of PKZ protein kinase at high NaCl concentration.

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[NMR paper] NMR elucidation of reduced B-Z transition activity of PKZ protein kinase at high NaCl concentration.

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NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

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Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

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RCI

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HADDOCK

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FANDAS

MestReS

V-NMR

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B-factor

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Sparta+

Camshift

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ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

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Shifty

Camcoil

Poulsen_rc_CS

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MAXOCC

Format conversion & validation:

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11-20-2016, 09:20 PM

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NMR elucidation of reduced B-Z transition activity of PKZ protein kinase at high NaCl concentration.

NMR elucidation of reduced B-Z transition activity of PKZ protein kinase at high NaCl concentration.

Related Articles NMR elucidation of reduced B-Z transition activity of PKZ protein kinase at high NaCl concentration.

Biochem Biophys Res Commun. 2016 Nov 14;:

Authors: Lee AR, Seo YJ, Choi SR, Ryu KS, Cheong HK, Lee SS, Park CJ, Lee JH

Abstract
A Z-DNA binding protein (ZBP)-containing protein kinase (PKZ) in fish species has an important role in the innate immune response. Previous structural studies of the Z? domain of the PKZ from Carassius auratus (caZ?PKZ) showed that the protein initially binds to B-DNA and induces B-Z transition of double stranded DNA in a salt concentration-dependent manner. However, the significantly reduced B-Z transition activity of caZ?PKZ at high salt concentration was not fully understood. In this study, we present the binding affinity of the protein for B-DNA and Z-DNA and characterize its extremely low B-Z transition activity at 250*mM NaCl. Our results emphasize that the B-DNA-bound form of caZ?PKZ can be used as molecular ruler to measure the degree of B-Z transition.

PMID: 27856245 [PubMed - as supplied by publisher]

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