NMR paper NMR characterization of native liquid spider dragline silk from Nephila edulis.

		BioNMR > Educational resources > Journal club
[NMR paper] NMR characterization of native liquid spider dragline silk from Nephila edulis.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

11-24-2010, 09:51 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,733

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

NMR characterization of native liquid spider dragline silk from Nephila edulis.

NMR characterization of native liquid spider dragline silk from Nephila edulis.

Related Articles NMR characterization of native liquid spider dragline silk from Nephila edulis.

Biomacromolecules. 2004 May-Jun;5(3):834-9

Authors: Hronska M, van Beek JD, Williamson PT, Vollrath F, Meier BH

Solid spider dragline silk is well-known for its mechanical properties. Nonetheless a detailed picture of the spinning process is lacking. Here we report NMR studies on the liquid silk within the wide sac of the major ampullate (m.a.) gland from the spider Nephila edulis. The resolution in the NMR spectra is shown to be significantly improved by the application of magic-angle spinning (MAS). From the narrow width of the resonance lines and the chemical shifts observed, it is concluded that the silk protein within the wide sac of the m.a. gland is dynamically disordered throughout the molecule in the sense that each amino acid of a given type senses an identical environment, on average. The NMR data obtained are consistent with an isotropic liquid phase.

PMID: 15132669 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
(13) C Solid state NMR study of the (13) C-labeled peptide, (E)(8) GGLGGQGAG(A)(6) GGAGQGGYGG as a model for the local structure of Nephila clavipes dragline silk (MaSp1) before and after spinning. (13) C Solid state NMR study of the (13) C-labeled peptide, (E)(8) GGLGGQGAG(A)(6) GGAGQGGYGG as a model for the local structure of Nephila clavipes dragline silk (MaSp1) before and after spinning. (13) C Solid state NMR study of the (13) C-labeled peptide, (E)(8) GGLGGQGAG(A)(6) GGAGQGGYGG as a model for the local structure of Nephila clavipes dragline silk (MaSp1) before and after spinning. Biopolymers. 2011 Sep 12; Authors: Yazawa K, Yamaguchi E, Knight D, Asakura T Abstract We prepared the water soluble model peptide, (E)(8)...	nmrlearner	Journal club	0	09-14-2011 08:07 PM
Elucidating metabolic pathways for amino acid incorporation into dragline spider silk using 13C enrichment and solid state NMR. Elucidating metabolic pathways for amino acid incorporation into dragline spider silk using 13C enrichment and solid state NMR. Elucidating metabolic pathways for amino acid incorporation into dragline spider silk using 13C enrichment and solid state NMR. Comp Biochem Physiol A Mol Integr Physiol. 2011 Jul;159(3):219-24 Authors: Creager MS, Izdebski T, Brooks AE, Lewis RV Abstract Spider silk has been evolutionarily optimized for contextual mechanical performance over the last 400 Ma. Despite precisely balanced mechanical properties,...	nmrlearner	Journal club	0	09-02-2011 05:40 PM
Solid-state NMR evidence for elastin-like beta-turn structure in spider dragline silk. Solid-state NMR evidence for elastin-like beta-turn structure in spider dragline silk. Solid-state NMR evidence for elastin-like beta-turn structure in spider dragline silk. Chem Commun (Camb). 2010 Sep 28;46(36):6714-6 Authors: Jenkins JE, Creager MS, Butler EB, Lewis RV, Yarger JL, Holland GP Two-dimensional homo- and heteronuclear solid-state MAS NMR experiments on (13)C/(15)N-proline labeled Argiope aurantia dragline silk provide evidence for an elastin-like beta-turn structure for the repetitive Gly-Pro-Gly-X-X motif prevalent in major...	nmrlearner	Journal club	0	12-28-2010 03:31 PM
[NMR paper] Design, expression and solid-state NMR characterization of silk-like materials constr Design, expression and solid-state NMR characterization of silk-like materials constructed from sequences of spider silk, Samia cynthia ricini and Bombyx mori silk fibroins. Related Articles Design, expression and solid-state NMR characterization of silk-like materials constructed from sequences of spider silk, Samia cynthia ricini and Bombyx mori silk fibroins. J Biochem. 2005 Jun;137(6):721-9 Authors: Yang M, Asakura T Silk has a long history of use in medicine as sutures. To address the requirements of a mechanically robust and...	nmrlearner	Journal club	0	11-25-2010 08:21 PM
[NMR paper] Dynamical characterization of residual and non-native structures in a partially folde Dynamical characterization of residual and non-native structures in a partially folded protein by (15)N NMR relaxation using a model based on a distribution of correlation times. Related Articles Dynamical characterization of residual and non-native structures in a partially folded protein by (15)N NMR relaxation using a model based on a distribution of correlation times. Protein Sci. 2002 Apr;11(4):957-64 Authors: Ochsenbein F, Neumann JM, Guittet E, van Heijenoort C A spectral density model based on a truncated lorentzian distribution of...	nmrlearner	Journal club	0	11-24-2010 08:49 PM
[NMR paper] Structural characterization of the molten globule and native states of ovalbumin: a 1 Structural characterization of the molten globule and native states of ovalbumin: a 1H NMR study. Related Articles Structural characterization of the molten globule and native states of ovalbumin: a 1H NMR study. J Pept Res. 1997 Dec;50(6):465-74 Authors: Sogami M, Era S, Koseki T, Nagai N Molecular characteristics of ovalbumin (OVA) in the acidic (pD 3.08, the E-form) and neutral regions were studied by measuring effective radii, 1H NMR spectra, spin-echo 1H NMR spectra and cross-relaxation times (TIS) from irradiated to observed protein...	nmrlearner	Journal club	0	08-22-2010 05:08 PM
[NMR paper] 13C NMR of Nephila clavipes major ampullate silk gland. 13C NMR of Nephila clavipes major ampullate silk gland. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-cellhub.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles 13C NMR of Nephila clavipes major ampullate silk gland. Biophys J. 1996 Dec;71(6):3442-7 Authors: Hijirida DH, Do KG, Michal C, Wong S, Zax D, Jelinski LW The major ampullate glands of the spider Nephila clavipes contain approximately 0.2 microliter each...	nmrlearner	Journal club	0	08-22-2010 02:20 PM
[NMR paper] Supercontracted spider dragline silk: a solid-state NMR study of the local structure. Supercontracted spider dragline silk: a solid-state NMR study of the local structure. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Supercontracted spider dragline silk: a solid-state NMR study of the local structure. Int J Biol Macromol. 1999 Mar-Apr;24(2-3):173-8 Authors: van Beek JD, Kümmerlen J, Vollrath F, Meier BH The local structure of supercontracted dragline silk from the spider Nephila madagascariensis was investigated by solid-state nuclear magnetic...	nmrlearner	Journal club	0	08-21-2010 04:03 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off