Physicochemical technologies are a powerful tool for the structural characterization of vaccine antigens both at bulk level as well as on the final formulation. High-field Nuclear Magnetic Resonance (NMR) spectroscopy has been found to be an extremely and robust tool for tracking the industrial process manufacturing of carbohydrate-based vaccines. I have applied NMR spectroscopy to the characterization of a penta-valent conjugate vaccine against Neisseria meninigitidis group A, C, W, Y (MenACWY)...
[NMR paper] Quantification of residual cetyltrimethylammonium bromide (CTAB) and sodium deoxycholate (DOC) in Haemophilus influenzae type b (Hib) polysaccharide using NMR
Quantification of residual cetyltrimethylammonium bromide (CTAB) and sodium deoxycholate (DOC) in Haemophilus influenzae type b (Hib) polysaccharide using NMR
CTAB and DOC are used as reagents in the purification of Hib polysaccharide. Polysaccharide is purified by precipitation with CTAB from fermented broth followed by solvent extraction and DOC is used to remove the protein impurities. The reagents used in the purification process should be removed from the product as per regulatory requirements. These two residual reagents can be easily identified and quantified in purified Haemophilus...
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02-24-2021 05:50 AM
[ASAP] Catalytic Cycle of Neisseria meningitidis CMP-Sialic Acid Synthetase Illustrated by High-Resolution Protein Crystallography
Catalytic Cycle of Neisseria meningitidis CMP-Sialic Acid Synthetase Illustrated by High-Resolution Protein Crystallography
https://pubs.acs.org/na101/home/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.9b00517/20191003/images/medium/bi9b00517_0009.gif
Biochemistry
DOI: 10.1021/acs.biochem.9b00517
http://feeds.feedburner.com/~r/acs/bichaw/~4/heIigCTU_BA
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02-29-2020 09:52 PM
An Atypical ?/?-Hydrolase Fold Revealedin the Crystal Structure of Pimeloyl-Acyl Carrier Protein Methyl EsteraseBioG from Haemophilus influenzae
An Atypical ?/?-Hydrolase Fold Revealedin the Crystal Structure of Pimeloyl-Acyl Carrier Protein Methyl EsteraseBioG from Haemophilus influenzae
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.6b00818/20161121/images/medium/bi-2016-00818f_0010.gif
Biochemistry
DOI: 10.1021/acs.biochem.6b00818
http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/bichaw/~4/CUC9biz-5ig
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11-21-2016 11:02 PM
[NMR paper] NMR assignment of intrinsically disordered self-processing module of the FrpC protein of Neisseria meningitidis.
NMR assignment of intrinsically disordered self-processing module of the FrpC protein of Neisseria meningitidis.
NMR assignment of intrinsically disordered self-processing module of the FrpC protein of Neisseria meningitidis.
Biomol NMR Assign. 2015 Jul 3;
Authors: Kubá? V, Nová?ek J, Bumba L, Žídek L
Abstract
The self-processing module (SPM) is an internal segment of the FrpC protein (P415-F591) secreted by the pathogenic Gram-negative bacterium Neisseria meningitidis during meningococcal infection of human upper respiratory...
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07-05-2015 02:07 AM
ASU center seeks potential vaccine for tularemia - Vaccine News Daily
http://www.bionmr.com//t3.gstatic.com/images?q=tbn:ANd9GcQ6qKuM7cxI6KDroGjcLdFAIb5kokLOd6-Ve2yUtfausAAPL6vw90fIm0s8-2aY8hSYZvCnQxil
Vaccine News Daily
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ASU center seeks potential vaccine for tularemia
Vaccine News Daily
Fromme and her ASU colleagues used nuclear magnetic resonance (NMR) spectroscopy to explore detailed features of the bacteria's surface protein. Their research marks the first structural determination at atomic scale for any lipoprotein of the genus ...
ASU center seeks potential vaccine for tularemia - Vaccine News Daily
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06-23-2015 11:58 PM
[NMR paper] Structural characterization by NMR of a double phosphorylated chimeric Peptide vaccine for treatment of Alzheimer's disease.
Structural characterization by NMR of a double phosphorylated chimeric Peptide vaccine for treatment of Alzheimer's disease.
Structural characterization by NMR of a double phosphorylated chimeric Peptide vaccine for treatment of Alzheimer's disease.
Molecules. 2013;18(5):4929-41
Authors: Ramírez-Gualito K, Richter M, Matzapetakis M, Singer D, Berger S
Abstract
Rational design of peptide vaccines becomes important for the treatment of some diseases such as Alzheimer's disease (AD) and related disorders. In this study, as part of a larger...
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04-30-2013 10:21 PM
[NMR paper] NMR structure of the hypothetical protein NMA1147 from Neisseria meningitidis reveals
NMR structure of the hypothetical protein NMA1147 from Neisseria meningitidis reveals a distinct 5-helix bundle.
Related Articles NMR structure of the hypothetical protein NMA1147 from Neisseria meningitidis reveals a distinct 5-helix bundle.
Proteins. 2004 May 15;55(3):756-8
Authors: Liu G, Sukumaran DK, Xu D, Chiang Y, Acton T, Goldsmith-Fischman S, Honig B, Montelione GT, Szyperski T
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11-24-2010 09:51 PM
[NMR paper] NMR assignment of the hypothetical protein HI0004 from Haemophilus influenzae--a puta
NMR assignment of the hypothetical protein HI0004 from Haemophilus influenzae--a putative essential gene product.
Related Articles NMR assignment of the hypothetical protein HI0004 from Haemophilus influenzae--a putative essential gene product.
J Biomol NMR. 2004 May;29(1):101-2
Authors: Cheon Yeh D, Parsons JF, Parsons LM, Liu F, Eisenstein E, Orban J
NMR characterization of a multi-valent conjugate vaccine against Neisseria meningitidis A, C, W, Y and Haemophilus influenzae b infections
Linear Mode
