Related ArticlesAn NMR biochemical assay for oxidosqualene cyclase: evaluation of inhibitor activities on Trypanosoma cruzi and human enzymes.
J Med Chem. 2018 May 17;:
Authors: Tani O, Akutsu Y, Ito S, Suzuki T, Tateishi Y, Yamaguchi T, Niimi T, Namatame I, Chiba Y, Sakashita H, Kubota T, Yanagi T, Mizukami S, Hirayama K, Furukawa K, Yamasaki K
Abstract
Oxidosqualene cyclase (OSC), a membrane-associated protein, is a key enzyme of sterol biosynthesis. Here we report a novel assay for OSC, involving reaction in aqueous solution, NMR quantification in organic solvent, and factor analysis of spectra. We evaluated one known and three novel inhibitors on OSC of Trypanosoma cruzi, a parasite causative of Chagas disease, and compared with their effects on human OSC for selectivity. Among them, one novel inhibitor showed a significant parasiticidal activity.
PMID: 29771525 [PubMed - as supplied by publisher]
[ASAP] Multiplexed Real-Time NMR GTPase Assay for Simultaneous Monitoring of Multiple Guanine Nucleotide Exchange Factor Activities from Human Cancer Cells and Organoids
Multiplexed Real-Time NMR GTPase Assay for Simultaneous Monitoring of Multiple Guanine Nucleotide Exchange Factor Activities from Human Cancer Cells and Organoids
Teklab Gebregiworgis, Christopher B. Marshall, Tadateru Nishikawa, Nikolina Radulovich, María-José Sandí, Zhenhao Fang, Robert Rottapel, Ming-Sound Tsao, Mitsuhiko Ikura
https://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.7b13703/20180322/images/medium/ja-2017-13703a_0005.gif
Journal of the American Chemical Society
DOI: 10.1021/jacs.7b13703...
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[NMR paper] Multiplexed real-time NMR GTPase assay for simultaneous monitoring of multiple GEF activities from human cancer cells and organoids.
Multiplexed real-time NMR GTPase assay for simultaneous monitoring of multiple GEF activities from human cancer cells and organoids.
Multiplexed real-time NMR GTPase assay for simultaneous monitoring of multiple GEF activities from human cancer cells and organoids.
J Am Chem Soc. 2018 Mar 15;:
Authors: Gebregiworgis T, Marshall CB, Nishikawa T, Radulovich N, Sandi MJ, Fang Z, Rottapel R, Tsao MS, Ikura M
Abstract
Small GTPases (sGTPases) are critical switch-like regulators that mediate several important cellular functions and...
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[NMR paper] c-MYC G-quadruplex binding by the RNA polymerase I inhibitor BMH-21 and analogues revealed by a combined NMR and biochemical Approach.
c-MYC G-quadruplex binding by the RNA polymerase I inhibitor BMH-21 and analogues revealed by a combined NMR and biochemical Approach.
Related Articles c-MYC G-quadruplex binding by the RNA polymerase I inhibitor BMH-21 and analogues revealed by a combined NMR and biochemical Approach.
Biochim Biophys Acta. 2017 Dec 08;:
Authors: Musso L, Mazzini S, Rossini A, Castagnoli L, Scaglioni L, Artali R, Di Nicola M, Zunino F, Dallavalle S
Abstract
BACKGROUND: Pyridoquinazolinecarboxamides have been reported as RNA polymerase I...
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Biochemical and Functional Evaluation of the IntramolecularDisulfide Bonds in the Zinc-Chelating Antimicrobial Protein HumanS100A7 (Psoriasin)
Biochemical and Functional Evaluation of the IntramolecularDisulfide Bonds in the Zinc-Chelating Antimicrobial Protein HumanS100A7 (Psoriasin)
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.7b00781/20171019/images/medium/bi-2017-00781d_0013.gif
Biochemistry
DOI: 10.1021/acs.biochem.7b00781
http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA
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[NMR paper] The soluble Y115E-Y117E variant of human glutaminyl cyclase is a valid target for X-ray and NMR screening of inhibitors against Alzheimer disease.
The soluble Y115E-Y117E variant of human glutaminyl cyclase is a valid target for X-ray and NMR screening of inhibitors against Alzheimer disease.
Related Articles The soluble Y115E-Y117E variant of human glutaminyl cyclase is a valid target for X-ray and NMR screening of inhibitors against Alzheimer disease.
Acta Crystallogr F Struct Biol Commun. 2015 Aug 1;71(Pt 8):986-92
Authors: DiPisa F, Pozzi C, Benvenuti M, Andreini M, Marconi G, Mangani S
Abstract
Recent developments in molecular pathology and genetics have allowed the...
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[NMR paper] NMR structure and dynamics of Q4D059, a kinetoplastid-specific and conserved protein from Trypanosoma cruzi.
NMR structure and dynamics of Q4D059, a kinetoplastid-specific and conserved protein from Trypanosoma cruzi.
Related Articles NMR structure and dynamics of Q4D059, a kinetoplastid-specific and conserved protein from Trypanosoma cruzi.
J Struct Biol. 2015 Mar 4;
Authors: López-Castilla A, Pons T, Pires JR
Abstract
Q4D059 (UniProt accession number), is an 86-residue protein from Trypanosoma cruzi, conserved in the related kinetoplastid parasites Trypanosoma brucei and Leishmania major. These pathogens are the causal agents of the...
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NMR structure and dynamics of Q4D059, a kinetoplastid-specific and conserved protein from Trypanosoma cruzi
NMR structure and dynamics of Q4D059, a kinetoplastid-specific and conserved protein from Trypanosoma cruzi
Publication date: Available online 4 March 2015
Source:Journal of Structural Biology</br>
Author(s): Aracelys López-Castilla , Tirso Pons , José R. Pires</br>
Q4D059 (UniProt accession number), is an 86-residue protein from Trypanosoma cruzi, conserved in the related kinetoplastid parasites Trypanosoma brucei and Leishmania major. These pathogens are the causal agents of the neglected diseases: Chagas, sleeping sickness and leishmaniases respectively and had...
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[NMR paper] NMR structural study of TcUBP1, a single RRM domain protein from Trypanosoma cruzi: c
NMR structural study of TcUBP1, a single RRM domain protein from Trypanosoma cruzi: contribution of a beta hairpin to RNA binding.
Related Articles NMR structural study of TcUBP1, a single RRM domain protein from Trypanosoma cruzi: contribution of a beta hairpin to RNA binding.
Biochemistry. 2005 Mar 15;44(10):3708-17
Authors: Volpon L, D'Orso I, Young CR, Frasch AC, Gehring K
TcUBP1 is a trypanosome cytoplasmic RNA-binding protein containing a single, conserved RNA-recognition motif (RRM) domain involved in selective destabilization of U-rich...
An NMR biochemical assay for oxidosqualene cyclase: evaluation of inhibitor activities on Trypanosoma cruzi and human enzymes.
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