NMR-based solution structure of the Caulobacter crescentus ProXp-ala trans-editing enzyme
 

NMR-based solution structure of the Caulobacter crescentus ProXp-ala trans-editing enzyme

ProXp-ala is a key component of the translational machinery in all three Domains of life. This enzyme helps to maintain the fidelity of proline codon translation through aminoacyl-tRNA^(Pro) proofreading. In the first step of tRNA aminoacylation, the cognate aminoacyl-tRNA synthetase (aaRS) binds and activates an amino acid in the enzyme's synthetic active site. If a non-cognate amino acid passes this first selection step and is charged onto the tRNA, a distinct aaRS editing active site may...

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