Related ArticlesNMR-based metabolomics of urine in a mouse model of Alzheimer's disease: identification of oxidative stress biomarkers.
J Clin Biochem Nutr. 2013 Mar;52(2):133-8
Authors: Fukuhara K, Ohno A, Ota Y, Senoo Y, Maekawa K, Okuda H, Kurihara M, Okuno A, Niida S, Saito Y, Takikawa O
Abstract
Alzheimer's disease (AD) is the most common cause of neurodegenerative dementia among elderly patients. A biomarker for the disease could make diagnosis easier and more accurate, and accelerate drug discovery. In this study, NMR-based metabolomics analysis in conjunction with multivariate statistics was applied to examine changes in urinary metabolites in transgenic AD mice expressing mutant tau and ?-amyloid precursor protein. These mice showed significant changes in urinary metabolites throughout the progress of the disease. Levels of 3-hydroxykynurenine, homogentisate and allantoin were significantly higher compared to control mice in 4 months (prior to onset of AD symptoms) and reverted to control values by 10 months of age (early/middle stage of AD), which highlights the relevance of oxidative stress to this neurodegenerative disorder even prior the onset of dementia. The level of these changed metabolites at very early period may provide an indication of disease risk at asymptomatic stage.
Alzheimer's analysed: New clues in brain disease
Alzheimer's analysed: New clues in brain disease
http://www.spectroscopynow.com/common/images/thumbnails/13af4d7f690.jpgRecent studies have added weight to the idea that amyloid-beta is a causative agent in Alzheimer's disease rather than the plaques formed from these chunks of misfolded peptide that deposit in the diseased brain. Now, European researchers have used REAPDOR solid-state NMR spectroscopy to analyse the hydrogen bonds in the tiny fibrils of amyloid-beta peptide, offering another perspective on this neurodegenerative disorder.
Source: Spectroscopynow.com
nmrlearner
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02-03-2013 08:49 AM
1H NMR Metabolomics Study of Human Urine
1H NMR Metabolomics Study of Human Urine
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1H NMR Metabolomics Study of Human Urine
A presentation of some recent work from the Metabolomics and Bioinformatics & Statistics Groups at the Institute of Food Research, Norwich, UK. See the teams' webpages at: www.ifr.ac.uk and www.ifr.ac.uk This video is a shortened version of a presentation given at "Advances in Metabolic Profiling 2011".
From:BioStatsXtra
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nmrlearner
NMR educational videos
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07-30-2012 07:42 AM
Identification of Cryptic Products of the Gliotoxin Gene Cluster Using NMR-Based Comparative Metabolomics and a Model for Gliotoxin Biosynthesis
Identification of Cryptic Products of the Gliotoxin Gene Cluster Using NMR-Based Comparative Metabolomics and a Model for Gliotoxin Biosynthesis
Ry R. Forseth, Ellen M. Fox, DaWoon Chung, Barbara J. Howlett, Nancy P. Keller and Frank C. Schroeder
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja2029987/aop/images/medium/ja-2011-029987_0004.gif
Journal of the American Chemical Society
DOI: 10.1021/ja2029987
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/kET0fe1YTXQ
nmrlearner
Journal club
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06-07-2011 01:01 AM
Detection of autosomal dominant polycystic kidney disease by NMR spectroscopic fingerprinting of urine.
Detection of autosomal dominant polycystic kidney disease by NMR spectroscopic fingerprinting of urine.
Detection of autosomal dominant polycystic kidney disease by NMR spectroscopic fingerprinting of urine.
Kidney Int. 2011 Mar 9;
Authors: Gronwald W, Klein MS, Zeltner R, Schulze BD, Reinhold SW, Deutschmann M, Immervoll AK, Böger CA, Banas B, Eckardt KU, Oefner PJ
Autosomal dominant polycystic kidney disease (ADPKD) is a frequent cause of kidney failure; however, urinary biomarkers for the disease are lacking. In a step towards identifying such...
nmrlearner
Journal club
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03-11-2011 03:14 PM
The application of micro-coil NMR probe technology to metabolomics of urine and serum
The application of micro-coil NMR probe technology to metabolomics of urine and serum
Abstract Increasing the sensitivity and throughput of NMR-based metabolomics is critical for the continued growth of this field. In this paper the application of micro-coil NMR probe technology was evaluated for this purpose. The most commonly used biofluids in metabolomics are urine and serum. In this study we examine different sample limited conditions and compare the detection sensitivity of the micro-coil with a standard 5 mm NMR probe. Sample concentration is evaluated as a means to leverage the...
nmrlearner
Journal club
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03-09-2011 04:19 AM
1H NMR metabolomics identification of markers of hypoxia-induced metabolic shifts in a breast cancer model system
1H NMR metabolomics identification of markers of hypoxia-induced metabolic shifts in a breast cancer model system
Abstract Hypoxia can promote invasive behavior in cancer cells and alters the response to therapeutic intervention as a result of changes in the expression many genes, including genes involved in intermediary metabolism. Although metabolomics technologies are capable of simultaneously measuring a wide range of metabolites in an untargeted manner, these methods have been relatively under utilized in the study of cancer cell responses to hypoxia. Thus, 1H NMR metabolomics was...
nmrlearner
Journal club
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03-09-2011 04:19 AM
(1)H NMR metabolomics identification of markers of hypoxia-induced metabolic shifts in a breast cancer model system.
(1)H NMR metabolomics identification of markers of hypoxia-induced metabolic shifts in a breast cancer model system.
(1)H NMR metabolomics identification of markers of hypoxia-induced metabolic shifts in a breast cancer model system.
J Biomol NMR. 2011 Mar 4;
Authors: Weljie AM, Bondareva A, Zang P, Jirik FR
Hypoxia can promote invasive behavior in cancer cells and alters the response to therapeutic intervention as a result of changes in the expression many genes, including genes involved in intermediary metabolism. Although metabolomics...
nmrlearner
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03-05-2011 01:02 PM
Study of acute biochemical effects of thallium toxicity in mouse urine by NMR spectroscopy.
Study of acute biochemical effects of thallium toxicity in mouse urine by NMR spectroscopy.
Study of acute biochemical effects of thallium toxicity in mouse urine by NMR spectroscopy.
J Appl Toxicol. 2011 Jan 7;
Authors: Tyagi R, Rana P, Khan AR, Bhatnagar D, Devi MM, Chaturvedi S, Tripathi RP, Khushu S
Thallium (Tl) is a toxic heavy metal and its exposure to the human body causes physiological and biochemical changes due to its interference with potassium-dependent biological reactions. A high-resolution (1) H NMR spectroscopy based metabonomic...
NMR-based metabolomics of urine in a mouse model of Alzheimer's disease: identification of oxidative stress biomarkers.
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