Related ArticlesNMR-based metabolomics and breath studies show lipid and protein catabolism during low dose chronic T1 AM treatment.
Obesity (Silver Spring). 2013 Mar 20;
Authors: Haviland JA, Reiland H, Butz DE, Tonelli M, Porter WP, Zucchi R, Scanlan TS, Chiellini G, Assadi-Porter FM
Abstract
OBJECTIVE: 3-iodothyronamine (T1 AM), an analog of thyroid hormone,is a recently discovered fast-acting endogenous metabolite. High single dose treatments of T1 AM have produced rapid short-term effects, including a reduction of body temperature, bradycardia, and hyperglycemia in mice. DESIGN AND METHODS: The present study monitored the effect of daily low doses of T1 AM (10mg/Kg) for eight-days on weight loss and metabolism in spontaneously overweight mice. The experiments were repeated twice (n=4). Nuclear magnetic resonance (NMR) spectroscopy of plasma and real-time analysis of exhaled (13) CO2 in breath by cavity ringdown spectroscopy (CRDS) were used to detect T1 M-induced lipolysis. RESULTS: CRDS detected increased lipolysis in breathshortly after T1 AM administration that was associated with a significant weight loss but independent of food consumption. NMR spectroscopy revealed alterations in key metabolites in serum: valine, glycine, and 3-hydroxybutyrate, suggesting that the subchronic effects of T1 AM include both lipolysis and protein breakdown. After discontinuation of T1 AM treatment, mice regained only 1.8% of the lost weight in the following two weeks, indicating lasting effects of T1 AM on weight maintenance. CONCLUSIONS: CRDS in combination with NMR and (13) C-metabolic tracing constitute a powerful method of investigation in obesity studies for identifying in vivo biochemical pathway shifts and unanticipated debilitating side effects.
PMID: 23512955 [PubMed - as supplied by publisher]
NMR-based metabolomics of mammalian cell and tissue cultures
NMR-based metabolomics of mammalian cell and tissue cultures
Abstract NMR spectroscopy was used to evaluate growth media and the cellular metabolome in two systems of interest to biomedical research. The first of these was a Chinese hamster ovary cell line engineered to express a recombinant protein. Here, NMR spectroscopy and a quantum mechanical total line shape analysis were utilized to quantify 30 metabolites such as amino acids, Krebs cycle intermediates, activated sugars, cofactors, and others in both media and cell extracts. The impact of bioreactor scale and addition of...
nmrlearner
Journal club
0
03-09-2011 04:19 AM
NMR-based metabolomics of mammalian cell and tissue cultures.
NMR-based metabolomics of mammalian cell and tissue cultures.
NMR-based metabolomics of mammalian cell and tissue cultures.
J Biomol NMR. 2011 Mar 4;
Authors: Aranibar N, Borys M, Mackin NA, Ly V, Abu-Absi N, Abu-Absi S, Niemitz M, Schilling B, Li ZJ, Brock B, Russell RJ, Tymiak A, Reily MD
NMR spectroscopy was used to evaluate growth media and the cellular metabolome in two systems of interest to biomedical research. The first of these was a Chinese hamster ovary cell line engineered to express a recombinant protein. Here, NMR spectroscopy and a...
nmrlearner
Journal club
0
03-05-2011 01:02 PM
NMR-based stable isotope resolved metabolomics in systems biochemistry
NMR-based stable isotope resolved metabolomics in systems biochemistry
Abstract An important goal of metabolomics is to characterize the changes in metabolic networks in cells or various tissues of an organism in response to external perturbations or pathologies. The profiling of metabolites and their steady state concentrations does not directly provide information regarding the architecture and fluxes through metabolic networks. This requires tracer approaches. NMR is especially powerful as it can be used not only to identify and quantify metabolites in an unfractionated mixture such...
nmrlearner
Journal club
0
03-03-2011 02:06 AM
Nuclear Magnetic Resonance (NMR)-Based Metabolomics.
Nuclear Magnetic Resonance (NMR)-Based Metabolomics.
Nuclear Magnetic Resonance (NMR)-Based Metabolomics.
Methods Mol Biol. 2011;708:321-34
Authors: Keun HC, Athersuch TJ
Biofluids are by far the most commonly studied sample type in metabolic profiling studies, encompassing blood, urine, cerebrospinal fluid, cell culture media and many others. A number of these fluids can be obtained at a high sampling frequency with minimal invasion, permitting detailed characterisation of dynamic metabolic events. One of the attractive properties of...
NMR-based metabolomics and breath studies show lipid and protein catabolism during low dose chronic T1 AM treatment.
Linear Mode
