Related ArticlesNMR-based metabolomic profile of hypercholesterolemic human sera: Relationship with in vitro gene expression?
PLoS One. 2020;15(4):e0231506
Authors: Grimaldi M, Palisi A, Marino C, Montoro P, Capasso A, Novi S, Tecce MF, D'Ursi AM
Abstract
Hypercholesterolaemia is considered an important cause of atherosclerotic cardiovascular disease. In a previous investigation, we demonstrated that cultured hepatoma cells treated with hypercholesterolaemic sera compared with cells treated with normocholesterolaemic sera show overexpression of mRNAs related to mitochondrial 3-hydroxy-3-methylglutaryl-coenzyme A synthase (HMGCS2). In the present work, using an NMR metabolomic analysis, we demonstrate that the hypercholesterolaemic blood sera previously used to treat cultured hepatoma cells are characterized by a metabolomic profile that is significantly different from the normocholesterolaemic sera. Acetate, acetone, 2-hydroxybutyrate, cysteine, valine, and glutamine are the metabolites distinguishing the two groups. Abnormalities in the concentrations of these metabolites reflect alterations in energy-related pathways, such as pantothenate and CoA biosynthesis, pyruvate, glycolysis/gluconeogenesis, the citrate cycle, and ketone bodies. Regarding ketone bodies, the pathway is regulated by HMGCS2; therefore, serum samples previously found to be able to increase HMGCS2 mRNA levels in cultured cells also contain higher amounts of the metabolites of its encoded enzyme protein product.
[NMR paper] Relationship between recombinant protein expression and host metabolome as determined by two-dimensional NMR spectroscopy.
Relationship between recombinant protein expression and host metabolome as determined by two-dimensional NMR spectroscopy.
Related Articles Relationship between recombinant protein expression and host metabolome as determined by two-dimensional NMR spectroscopy.
PLoS One. 2017;12(5):e0177233
Authors: Chae YK, Kim SH, Markley JL
Abstract
Escherichia coli has been the most widely used host to produce large amounts of heterologous proteins. However, given an input plasmid DNA, E. coli may produce soluble protein, produce only...
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[NMR paper] In*vitro mutagenicity, NMR metabolite characterization of azo and triphenylmethanes dyes by adherents bacteria and the role of the "cna" adhesion gene in activated sludge.
In*vitro mutagenicity, NMR metabolite characterization of azo and triphenylmethanes dyes by adherents bacteria and the role of the "cna" adhesion gene in activated sludge.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles In*vitro mutagenicity, NMR metabolite characterization of azo and triphenylmethanes dyes by adherents bacteria and the role of the "cna" adhesion gene in activated sludge.
Microb Pathog. 2017 Feb;103:29-39
Authors: Ayed L, Bakir K, Ben Mansour...
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Students bring 'fresh insights' to scientific research on gene expression, deep neural networks and more - Clark University News Hub
http://www.bionmr.com//t1.gstatic.com/images?q=tbn:ANd9GcTVd062HSNkufpFLOXoHxhOr1s0onXKhPyi91bbXm51srwsuet1eajYCvbuLO7yooXH_P0TNwUX
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Students bring 'fresh insights' to scientific research on gene expression, deep neural networks and more
Clark University News Hub
... biochemistry and molecular biology senior Pinky Htun '17 of Myanmar spent June and July isolating, purifying and studying proteins using state-of-the-art lab equipment, including a high-speed centrifuge and a nuclear magnetic resonance spectrometer.
Students...
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08-25-2016 05:42 AM
[NMR paper] A Facile method for expression and purification of (15)N isotope-labeled human Alzheimer's ?-amyloid peptides from E. coli for NMR-based structural analysis.
A Facile method for expression and purification of (15)N isotope-labeled human Alzheimer's ?-amyloid peptides from E. coli for NMR-based structural analysis.
Related Articles A Facile method for expression and purification of (15)N isotope-labeled human Alzheimer's ?-amyloid peptides from E. coli for NMR-based structural analysis.
Protein Expr Purif. 2015 Jul 28;
Authors: Sharma SC, Armand T, Aurelia Ball K, Chen A, Pelton JG, Wemmer DE, Head-Gordon T
Abstract
Alzheimer's disease (AD) is a progressive neurodegenerative disease...
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08-02-2015 08:22 PM
[NMR paper] Depletion of casein kinase I leads to a NAD(P)(+)/NAD(P)H balance-dependent metabolic adaptation as determined by NMR spectroscopy-metabolomic profile in Kluyveromyces lactis.
Depletion of casein kinase I leads to a NAD(P)(+)/NAD(P)H balance-dependent metabolic adaptation as determined by NMR spectroscopy-metabolomic profile in Kluyveromyces lactis.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Depletion of casein kinase I leads to a NAD(P)(+)/NAD(P)H balance-dependent metabolic adaptation as determined by NMR spectroscopy-metabolomic profile in Kluyveromyces lactis.
Biochim Biophys Acta. 2014 Jan;1840(1):556-64
Authors: Gorietti D, Zanni...
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High-yield Escherichia coli-based cell-free expression of human proteins
High-yield Escherichia coli-based cell-free expression of human proteins
Abstract Production of sufficient amounts of human proteins is a frequent bottleneck in structural biology. Here we describe an Escherichia coli-based cell-free system which yields mg-quantities of human proteins in N-terminal fusion constructs with the GB1 domain, which show significantly increased translation efficiency. A newly generated E. coli BL21 (DE3) RIPL-Star strain was used, which contains a variant RNase E with reduced activity and an excess of rare-codon tRNAs, and is devoid of lon and ompT protease...
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03-17-2012 07:32 AM
Expression and purification of (15)N- and (13)C-isotope labeled 40-residue human Alzheimer's ?-amyloid peptide for NMR-based structural analysis.
Expression and purification of (15)N- and (13)C-isotope labeled 40-residue human Alzheimer's ?-amyloid peptide for NMR-based structural analysis.
Expression and purification of (15)N- and (13)C-isotope labeled 40-residue human Alzheimer's ?-amyloid peptide for NMR-based structural analysis.
Protein Expr Purif. 2011 May 27;
Authors: Long F, Cho W, Ishii Y
Amyloid fibrils of Alzheimer's ?-amyloid peptide (A?) are a primary component of amyloid plaques, a hallmark of Alzheimer's disease (AD). Enormous attention has been given to the structural...
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06-07-2011 11:05 AM
[NMR paper] Recombinant gene expression and 1H NMR characteristics of the kringle (2 + 3) supermo
Recombinant gene expression and 1H NMR characteristics of the kringle (2 + 3) supermodule: spectroscopic/functional individuality of plasminogen kringle domains.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles Recombinant gene expression and 1H NMR characteristics of the kringle (2 + 3) supermodule: spectroscopic/functional individuality of plasminogen kringle domains.
Biochemistry. 1996 Feb 20;35(7):2357-64
Authors: Söhndel S, Hu CK, Marti D, Affolter M, Schaller J, Llinás M, Rickli EE
...
NMR-based metabolomic profile of hypercholesterolemic human sera: Relationship with in vitro gene expression?
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