NMR-based metabolite profiling of human milk: a pilot study of methods for investigating compositional changes during lactation.
Biochem Biophys Res Commun. 2015 Dec 1;
Authors: Wu J, Domellöf M, Zivkovic AM, Larsson G, Öhman A, Nording ML
Abstract
Low-molecular-weight metabolites in human milk are gaining increasing interest in studies of infant nutrition. In the present study, the milk metabolome from a single mother was explored at different stages of lactation. Metabolites were extracted from sample aliquots using either methanol/water (MeOH/H2O) extraction or ultrafiltration. Nuclear magnetic resonance (NMR) spectroscopy was used for metabolite identification and quantification, and multi- and univariate statistical data analyses were used to detect changes over time of lactation. Compared to MeOH/H2O extraction, ultrafiltration more efficiently reduced the interference from lipid and protein resonances, thereby enabling the identification and quantification of 36 metabolites. The human milk metabolomes at the early (9 - 24 days after delivery) and late (31 - 87 days after delivery) stages of lactation were distinctly different according to multi- and univariate statistics. The late lactation stage is characterized by significantly elevated concentrations of lactose, choline, alanine, glutamate, and glutamine, as well as by reduced levels of citrate, phosphocholine, glycerophosphocholine, and N-acetylglucosamine. Our results indicate that there are significant compositional changes of the human milk metabolome also in different phases of the matured lactation stage. These findings complement temporal studies on the colostrum and transitional metabolome in providing a better understanding of the nutritional variations received by an infant.
PMID: 26655810 [PubMed - as supplied by publisher]
[NMR paper] A pilot metabolic profiling study in hepatopancreas of Litopenaeus vannamei with white spot syndrome virus based on (1)H NMR spectroscopy.
A pilot metabolic profiling study in hepatopancreas of Litopenaeus vannamei with white spot syndrome virus based on (1)H NMR spectroscopy.
A pilot metabolic profiling study in hepatopancreas of Litopenaeus vannamei with white spot syndrome virus based on (1)H NMR spectroscopy.
J Invertebr Pathol. 2014 Oct 14;124C:51-56
Authors: Liu PF, Liu QH, Wu Y, Jie H
Abstract
White spot syndrome virus, which was a pathogen first found in 1992, had emerged globally affecting shrimp populations in aquaculture. Here, we comprehensively...
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[NMR paper] Rapid milk group classification by 1H NMR analysis of Le and H epitopes in human milk oligosaccharide donor samples.
Rapid milk group classification by 1H NMR analysis of Le and H epitopes in human milk oligosaccharide donor samples.
Related Articles Rapid milk group classification by 1H NMR analysis of Le and H epitopes in human milk oligosaccharide donor samples.
Glycobiology. 2014 Apr 29;
Authors: van Leeuwen SS, Schoemaker RJ, Gerwig GJ, van Leusen-van Kan EJ, Dijkhuizen L, Kamerling JP
Abstract
Human milk oligosaccharides (HMOs) are a major constituent of human breast milk and play an important role in reducing the risk of infections in...
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05-03-2014 10:42 PM
[NMR paper] Novel methods based on 13C detection to study intrinsically disordered proteins
Novel methods based on 13C detection to study intrinsically disordered proteins
Publication date: April 2014
Source:Journal of Magnetic Resonance, Volume 241</br>
Author(s): Isabella C. Felli , Roberta Pierattelli</br>
Intrinsically disordered proteins (IDPs) are characterized by highly flexible solvent exposed backbones and can sample many different conformations. These properties confer them functional advantages, complementary to those of folded proteins, which need to be characterized to expand our view of how protein structural and dynamic features affect...
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03-22-2014 01:28 AM
[NMR paper] (1)H NMR-Based Metabolite Profiling of Plasma in a Rat Model of Chronic Kidney Disease.
(1)H NMR-Based Metabolite Profiling of Plasma in a Rat Model of Chronic Kidney Disease.
(1)H NMR-Based Metabolite Profiling of Plasma in a Rat Model of Chronic Kidney Disease.
PLoS One. 2014 Jan 20;9(1):e85445
Authors: Kim JA, Choi HJ, Kwon YK, Ryu do H, Kwon TH, Hwang GS
Abstract
Chronic kidney disease (CKD) is characterized by the gradual loss of the kidney function to excrete wastes and fluids from the blood. (1)H NMR-based metabolomics was exploited to investigate the altered metabolic pattern in rats with CKD induced by surgical...
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01-28-2014 11:53 AM
[NMR paper] 1H NMR Based Targeted Metabolite Profiling for Understanding the Complex Relationship Connecting Oxidative Stress with Endometriosis.
1H NMR Based Targeted Metabolite Profiling for Understanding the Complex Relationship Connecting Oxidative Stress with Endometriosis.
1H NMR Based Targeted Metabolite Profiling for Understanding the Complex Relationship Connecting Oxidative Stress with Endometriosis.
Biomed Res Int. 2013;2013:329058
Authors: Jana SK, Dutta M, Joshi M, Srivastava S, Chakravarty B, Chaudhury K
Abstract
Accumulating evidence indicates the active role of oxidative stress in the development of endometriosis; however, the mechanism of reactive oxygen species...
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08-29-2013 01:53 PM
[NMR paper] Comparison of GC-MS and NMR for metabolite profiling of rice subjected to submergence stress.
Comparison of GC-MS and NMR for metabolite profiling of rice subjected to submergence stress.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Comparison of GC-MS and NMR for metabolite profiling of rice subjected to submergence stress.
J Proteome Res. 2013 Feb 1;12(2):898-909
Authors: Barding GA, Béni S, Fukao T, Bailey-Serres J, Larive CK
Abstract
Natural disasters such as drought, extreme temperatures, and flooding can severely impact crop production....
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07-06-2013 09:57 PM
Nuclear magnetic resonance (NMR)-based drug metabolite profiling.
Nuclear magnetic resonance (NMR)-based drug metabolite profiling.
Nuclear magnetic resonance (NMR)-based drug metabolite profiling.
Methods Mol Biol. 2011;708:299-319
Authors: Lenz EM
The identification of drug metabolites in biofluids such as urine, plasma and bile is an important step in drug discovery and development. Proton nuclear magnetic resonance ((1)H-NMR) spectroscopy can provide detailed information regarding the structural transformation of a compound as a consequence of metabolism. However, successful identification of drug...
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[NMR paper] In vivo 13C NMR metabolite profiling: potential for understanding and assessing conifer seed quality.
In vivo 13C NMR metabolite profiling: potential for understanding and assessing conifer seed quality.
Related Articles In vivo 13C NMR metabolite profiling: potential for understanding and assessing conifer seed quality.
J Exp Bot. 2005 Aug;56(418):2253-65
Authors: Terskikh VV, Feurtado JA, Borchardt S, Giblin M, Abrams SR, Kermode AR
High-resolution 13C MAS NMR spectroscopy was used to profile a range of primary and secondary metabolites in vivo in intact whole seeds of eight different conifer species native to North America, including six of...
NMR-based metabolite profiling of human milk: a pilot study of methods for investigating compositional changes during lactation.
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