Related ArticlesNMR-Based Metabolic Profiling Reveals Neurochemical Alterations in the Brain of Rats Treated with Sorafenib.
Neurotox Res. 2015 Nov;28(4):290-301
Authors: Du C, Shao X, Zhu R, Li Y, Zhao Q, Fu D, Gu H, Kong J, Luo L, Long H, Deng P, Wang H, Hu C, Zhao Y, Cen X
Abstract
Sorafenib, an active multi-kinase inhibitor, has been widely used as a chemotherapy drug to treat advanced clear-cell renal cell carcinoma patients. In spite of the relative safety, sorafenib has been shown to exert a negative impact on cognitive functioning in cancer patients, specifically on learning and memory; however, the underlying mechanism remains unclear. In this study, an NMR-based metabolomics approach was applied to investigate the neurochemical effects of sorafenib in rats. Male rats were once daily administrated with 120 mg/kg sorafenib by gavage for 3, 7, and 28 days, respectively. NMR-based metabolomics coupled with histopathology examinations for hippocampus, prefrontal cortex (PFC), and striatum were performed. The (1)H NMR spectra data were analyzed by using multivariate pattern recognition techniques to show the time-dependent biochemical variations induced by sorafenib. Excellent separation was obtained and distinguishing metabolites were observed between sorafenib-treated and control rats. A total of 36 differential metabolites in hippocampus of rats treated with sorafenib were identified, some of which were significantly changed. Furthermore, these modified metabolites mainly reflected the disturbances in neurotransmitters, energy metabolism, membrane, and amino acids. However, only a few metabolites in PFC and striatum were altered by sorafenib. Additionally, no apparent histological changes in these three brain regions were observed in sorafenib-treated rats. Together, our findings demonstrate the disturbed metabonomics pathways, especially, in hippocampus, which may underlie the sorafenib-induced cognitive deficits in patients. This work also shows the advantage of NMR-based metabolomics over traditional approach on the study of biochemical effects of drugs.
[NMR paper] Pea Fiber and Wheat Bran Fiber Show Distinct Metabolic Profiles in Rats as Investigated by a 1H NMR-Based Metabolomic Approach.
Pea Fiber and Wheat Bran Fiber Show Distinct Metabolic Profiles in Rats as Investigated by a 1H NMR-Based Metabolomic Approach.
Pea Fiber and Wheat Bran Fiber Show Distinct Metabolic Profiles in Rats as Investigated by a 1H NMR-Based Metabolomic Approach.
PLoS One. 2014;9(12):e115561
Authors: Liu G, Xiao L, Fang T, Cai Y, Jia G, Zhao H, Wang J, Chen X, Wu C
Abstract
This study aimed to examine the effect of pea fiber (PF) and wheat bran fiber (WF) supplementation in rat metabolism. Rats were assigned randomly to one of three...
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12-30-2014 03:28 PM
[NMR paper] A pilot metabolic profiling study in hepatopancreas of Litopenaeus vannamei with white spot syndrome virus based on (1)H NMR spectroscopy.
A pilot metabolic profiling study in hepatopancreas of Litopenaeus vannamei with white spot syndrome virus based on (1)H NMR spectroscopy.
A pilot metabolic profiling study in hepatopancreas of Litopenaeus vannamei with white spot syndrome virus based on (1)H NMR spectroscopy.
J Invertebr Pathol. 2014 Oct 14;124C:51-56
Authors: Liu PF, Liu QH, Wu Y, Jie H
Abstract
White spot syndrome virus, which was a pathogen first found in 1992, had emerged globally affecting shrimp populations in aquaculture. Here, we comprehensively...
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12-03-2014 04:05 PM
Journal Highlight: 1H NMR based metabolic profiling in Crohn's disease by random forest methodology
Journal Highlight: 1H NMR based metabolic profiling in Crohn's disease by random forest methodology
http://www.spectroscopynow.com/common/images/thumbnails/146ed5b84e7.jpg1H NMR spectroscopy of the serum metabolites of patients with Crohn's disease, supported by random forest methodology, showed that valine and isoleucine are differentiating metabolites for diagnosis.
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06-30-2014 11:39 PM
[NMR paper] Behavioral, neurochemical and morphological changes induced by the overexpression of munc18-1a in brain of mice: relevance to schizophrenia.
Behavioral, neurochemical and morphological changes induced by the overexpression of munc18-1a in brain of mice: relevance to schizophrenia.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.nature.com-images-lo_tp.gif Related Articles Behavioral, neurochemical and morphological changes induced by the overexpression of munc18-1a in brain of mice: relevance to schizophrenia.
Transl Psychiatry. 2013;3:e221
Authors: Urigüen L, Gil-Pisa I, Munarriz-Cuezva E, Berrocoso E, Pascau J, Soto-Montenegro ML, Gutiérrez-Adán A, Pintado B, Madrigal JL, Castro E,...
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02-03-2013 10:19 AM
Metabolic profiling of cadmium-induced effects in one pioneer intertidal halophyte Suaeda salsa by NMR-based metabolomics.
Metabolic profiling of cadmium-induced effects in one pioneer intertidal halophyte Suaeda salsa by NMR-based metabolomics.
Metabolic profiling of cadmium-induced effects in one pioneer intertidal halophyte Suaeda salsa by NMR-based metabolomics.
Ecotoxicology. 2011 May 15;
Authors: Liu X, Yang C, Zhang L, Li L, Liu S, Yu J, You L, Zhou D, Xia C, Zhao J, Wu H
Cadmium is a non-essential element to living organisms and has become the severe contaminant in both seawater and sediment in the intertidal zones of the Bohai Sea. The halophyte, Suaeda salsa...
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05-17-2011 06:21 PM
1H NMR-based metabolic profiling reveals inherent biological variation in yeast and nematode model systems
1H NMR-based metabolic profiling reveals inherent biological variation in yeast and nematode model systems
Abstract The application of metabolomics to human and animal model systems is poised to provide great insight into our understanding of disease etiology and the metabolic changes that are associated with these conditions. However, metabolomic studies have also revealed that there is significant, inherent biological variation in human samples and even in samples from animal model systems where the animals are housed under carefully controlled conditions. This inherent biological...
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03-03-2011 02:06 AM
In Vivo NMR Metabolic Profiling of Fabrea salina Reveals Sequential Defense Mechanisms against Ultraviolet Radiation.
In Vivo NMR Metabolic Profiling of Fabrea salina Reveals Sequential Defense Mechanisms against Ultraviolet Radiation.
In Vivo NMR Metabolic Profiling of Fabrea salina Reveals Sequential Defense Mechanisms against Ultraviolet Radiation.
Biophys J. 2011 Jan 5;100(1):215-24
Authors: Marangoni R, Paris D, Melck D, Fulgentini L, Colombetti G, Motta A
Fabrea salina is a hypersaline ciliate that is known to be among the strongest ultraviolet (UV)-resistant microorganisms; however, the molecular mechanisms of this resistance are almost unknown. By means...
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12-31-2010 07:03 PM
[NMR paper] 31P-NMR of liver peroxisome membranes from normal and clofibrate-treated rats.
31P-NMR of liver peroxisome membranes from normal and clofibrate-treated rats.
Related Articles 31P-NMR of liver peroxisome membranes from normal and clofibrate-treated rats.
Cell Mol Biol (Noisy-le-grand). 1993 Jul;39(5):479-89
Authors: Serafini B, Cimini A, Sette M, Sartori C
Normal and clofibrate-induced rat liver peroxisomes were studied in order to correlate the drug-elicited modifications of membrane permeability and fluidity with changes of the phospholipid component. The phospholipid composition and membrane fluidity of purified...
NMR-Based Metabolic Profiling Reveals Neurochemical Alterations in the Brain of Rats Treated with Sorafenib.
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