NMR paper An NMR-based biosensor to measure stereo-specific methionine sulfoxide reductase (MSR) activities in vitro and in vivo.

		BioNMR > Educational resources > Journal club
[NMR paper] An NMR-based biosensor to measure stereo-specific methionine sulfoxide reductase (MSR) activities in vitro and in vivo.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

06-06-2020, 04:36 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

An NMR-based biosensor to measure stereo-specific methionine sulfoxide reductase (MSR) activities in vitro and in vivo.

An NMR-based biosensor to measure stereo-specific methionine sulfoxide reductase (MSR) activities in vitro and in vivo.

Related Articles An NMR-based biosensor to measure stereo-specific methionine sulfoxide reductase (MSR) activities in vitro and in vivo.

Chemistry. 2020 Jun 05;:

Authors: Sanchez-Lopez C, Labadie N, Lombardo V, Biglione F, Manta B, Jacob R, Gladyshev V, Abdelilah-Seyfried S, Selenko P, Binolfi A

Abstract
Oxidation of protein methionines to methionine-sulfoxides (MetOx) is associated with several age-related diseases. In healthy cells, MetOx is reduced to methionine by two families of conserved methionine sulfoxide reductase enzymes, MSRA and MSRB that specifically target the S - or R -diastereoisomers of methionine-sulfoxides, respectively. To directly interrogate MSRA and MSRB functions in cellular settings, we developed an NMR-based biosensor that we call CarMetOx to simultaneously measure both enzyme activities in single reaction setups. We demonstrate the suitability of our strategy to delineate MSR functions in complex biological environments, including cell lysates and live zebrafish embryos. Thereby, we establish differences in substrate specificities between prokaryotic and eukaryotic MSRs and introduce CarMetOx as a highly sensitive tool for studying therapeutic targets of oxidative stress-related human diseases and redox regulated signaling pathways.

PMID: 32501570 [PubMed - as supplied by publisher]

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Proximity-triggered covalent stabilization of low-affinity protein complexes in vitro and in vivo Proximity-triggered covalent stabilization of low-affinity protein complexes in vitro and in vivo The characterization of low-affinity protein complexes is challenging due to their dynamic nature. Here we present a method to stabilize transient protein complexes in vivo by generating a covalent and conformationally flexible bridge between the interaction partners. A highly active pyrrolysyl tRNA synthetase mutant directs the incorporation of unnatural amino acids bearing bromoalkyl moieties (BrCnK) into proteins. We demonstrate for the first time that low-affinity protein complexes...	nmrlearner	Journal club	0	09-28-2017 07:57 PM
Fumarase activity: an in vivo and in vitro biomarker for acute kidney injury From The DNP-NMR Blog: Fumarase activity: an in vivo and in vitro biomarker for acute kidney injury p.p1 {margin: 0.0px 0.0px 0.0px 36.0px; text-indent: -36.0px; font: 12.0px Helvetica} Nielsen, P.M., et al., Fumarase activity: an in vivo and in vitro biomarker for acute kidney injury. Scientific Reports, 2017. 7: p. 40812. http://dx.doi.org/10.1038/srep40812	nmrlearner	News from NMR blogs	0	05-09-2017 12:37 AM
[NMR paper] Orthogonal spin labeling using click chemistry for in vitro and in vivo applications Orthogonal spin labeling using click chemistry for in vitro and in vivo applications Publication date: Available online 2 December 2016 Source:Journal of Magnetic Resonance</br> Author(s): Svetlana Kucher, Sergei Korneev, Swati Tyagi, Ronja Apfelbaum, Dina Grohmann, Edward A. Lemke, Johann P. Klare, Heinz-Jürgen Steinhoff, Daniel Klose</br> Site-directed spin labeling for EPR- and NMR spectroscopy has mainly been achieved exploiting the specific reactivity of cysteines. For proteins with native cysteines or for in vivo applications, an alternative...	nmrlearner	Journal club	0	12-03-2016 02:09 PM
[NMR paper] A Multiplexed NMR-Reporter Approach to Measure Cellular Kinase and Phosphatase Activities in Real-Time. A Multiplexed NMR-Reporter Approach to Measure Cellular Kinase and Phosphatase Activities in Real-Time. http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles A Multiplexed NMR-Reporter Approach to Measure Cellular Kinase and Phosphatase Activities in Real-Time. J Am Chem Soc. 2015 May 27;137(20):6468-71 Authors: Thongwichian R, Kosten J, Benary U, Rose HM, Stuiver M, Theillet FX, Dose A, Koch B, Yokoyama H, Schwarzer D, Wolf J, Selenko P Abstract Cell...	nmrlearner	Journal club	0	03-02-2016 07:20 PM
A Multiplexed NMR-Reporter Approach to Measure Cellular Kinase and Phosphatase Activities in Real-Time A Multiplexed NMR-Reporter Approach to Measure Cellular Kinase and Phosphatase Activities in Real-Time Rossukon Thongwichian, Jonas Kosten, Uwe Benary, Honor May Rose, Marchel Stuiver, Francois-Xavier Theillet, Alexander Dose, Birgit Koch, Hideki Yokoyama, Dirk Schwarzer, Jana Wolf and Philipp Selenko http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.5b02987/20150515/images/medium/ja-2015-029876_0005.gif Journal of the American Chemical Society DOI: 10.1021/jacs.5b02987 http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA ...	nmrlearner	Journal club	0	05-16-2015 01:45 PM
Automated sequence- and stereo-specific assignment of methyl-labeled proteins by paramagnetic relaxation and methylâ??methyl nuclear overhauser enhancement spectroscopy Automated sequence- and stereo-specific assignment of methyl-labeled proteins by paramagnetic relaxation and methylâ??methyl nuclear overhauser enhancement spectroscopy Abstract Methyl-transverse relaxation optimized spectroscopy is rapidly becoming the preferred NMR technique for probing structure and dynamics of very large proteins up to ~1 MDa in molecular size. Data interpretation, however, necessitates assignment of methyl groups which still presents a very challenging and time-consuming process. Here we demonstrate that, in combination with a known 3D structure, paramagnetic...	nmrlearner	Journal club	0	09-26-2011 06:42 AM
Discovery of a potent and efficacious peptide derivative for ?/? opioid agonist/neurokinin 1 antagonist activity with a 2',6'-dimethyl-L-tyrosine: in vitro, in vivo, and NMR-based structural studies. Discovery of a potent and efficacious peptide derivative for ?/? opioid agonist/neurokinin 1 antagonist activity with a 2',6'-dimethyl-L-tyrosine: in vitro, in vivo, and NMR-based structural studies. Discovery of a potent and efficacious peptide derivative for ?/? opioid agonist/neurokinin 1 antagonist activity with a 2',6'-dimethyl-L-tyrosine: in vitro, in vivo, and NMR-based structural studies. J Med Chem. 2011 Apr 14;54(7):2029-38 Authors: Yamamoto T, Nair P, Largent-Milnes TM, Jacobsen NE, Davis P, Ma SW, Yamamura HI, Vanderah TW, Porreca F, Lai J, Hruby...	nmrlearner	Journal club	0	06-07-2011 11:05 AM
[NMR paper] Validation of a noninvasive method to measure brain temperature in vivo using 1H NMR Validation of a noninvasive method to measure brain temperature in vivo using 1H NMR spectroscopy. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_120x27.gif Related Articles Validation of a noninvasive method to measure brain temperature in vivo using 1H NMR spectroscopy. J Neurochem. 1995 Mar;64(3):1224-30 Authors: Corbett RJ, Laptook AR, Tollefsbol G, Kim B The goal of this study was to evaluate the potential of using the difference between the 1H NMR...	nmrlearner	Journal club	0	08-22-2010 03:41 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off