NMR assignments of actin depolymerizing factor (ADF) like UNC-60A and cofilin like UNC-60B proteins of Caenorhabditis elegans.
Biomol NMR Assign. 2014 Dec 11;
Authors: Shukla VK, Kabra A, Yadav R, Ono S, Kumar D, Arora A
Abstract
The actin filament dynamics in nematode, Caenorhabditis elegans, is regulated by differential activity of two proteins UNC-60A and UNC-60B. UNC-60A exhibits strong pointed end depolymerization on C. elegans actin (Ce-actin), strong inhibition of polymerization, strong monomer sequestering activity, weak severing activity, and low affinity for F-actin binding, while UNC-60B exhibits strong pointed end depolymerization on rabbit muscle actin, strong severing activity, and high affinity for F-actin binding. Structural characterization of these proteins will help to understand (1) molecular mechanism of actin dynamics regulation and (2) the differential activity of these proteins. Here, we report (1)H, (13)C, and (15)N chemical shift assignments of these two proteins as determined by heteronuclear NMR experiments (at pH 6.5 and temperature 298*K).
PMID: 25503290 [PubMed - as supplied by publisher]
[NMR paper] Backbone and side chain NMR assignments for the ribosome assembly factor Nop6 from Saccharomyces cerevisiae.
Backbone and side chain NMR assignments for the ribosome assembly factor Nop6 from Saccharomyces cerevisiae.
Backbone and side chain NMR assignments for the ribosome assembly factor Nop6 from Saccharomyces cerevisiae.
Biomol NMR Assign. 2013 Aug 7;
Authors: Wurm JP, Lioutikov A, Kötter P, Entian KD, Wöhnert J
Abstract
The Saccharomyces cerevisiae Nop6 protein is involved in the maturation of the small ribosomal subunit. It contains a central RNA binding domain and a predicted C-terminal coiled-coil domain. Here we report the almost...
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[NMR paper] Structural and Functional Insight into ADF/Cofilin from Trypanosoma brucei.
Structural and Functional Insight into ADF/Cofilin from Trypanosoma brucei.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.plosone.org-images-pone_120x30.png http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Structural and Functional Insight into ADF/Cofilin from Trypanosoma brucei.
PLoS One. 2013;8(1):e53639
Authors: Dai K, Liao S, Zhang J, Zhang X, Tu X
Abstract
The ADF/cofilin family has been characterized as a group of actin-binding...
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02-03-2013 10:19 AM
[NMR paper] NMR backbone assignments of the tyrosine kinase domain of human fibroblast growth factor receptor 1.
NMR backbone assignments of the tyrosine kinase domain of human fibroblast growth factor receptor 1.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles NMR backbone assignments of the tyrosine kinase domain of human fibroblast growth factor receptor 1.
Biomol NMR Assign. 2013 Jan 17;
Authors: Vajpai N, Schott AK, Vogtherr M, Breeze AL
Abstract
Members of the fibroblast growth factor receptor tyrosine kinase family (FGFR1-4) play an important role in many...
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NMR structure of the let-7 miRNA interacting with the site LCS1 of lin-41 mRNA from Caenorhabditis elegans.
NMR structure of the let-7 miRNA interacting with the site LCS1 of lin-41 mRNA from Caenorhabditis elegans.
NMR structure of the let-7 miRNA interacting with the site LCS1 of lin-41 mRNA from Caenorhabditis elegans.
Nucleic Acids Res. 2010 Nov 1;38(21):7814-21
Authors: Cevec M, Thibaudeau C, Plavec J
We have determined the 3D structure of a 34-nt RNA construct, herein named LCS1co, which mimics the interaction of let-7 microRNA (miRNA) to one of its complementary binding sites, LCS1, in the 3'-untranslated region of lin-41 mRNA by solution-state...
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[NMR paper] NMR assignments of the cold-shock protein ribosome-binding factor A (RbfA) from Therm
NMR assignments of the cold-shock protein ribosome-binding factor A (RbfA) from Thermotoga maritima.
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J Biomol NMR. 2005 Jan;31(1):73-4
Authors: Grimm SK, Wöhnert J
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[NMR paper] NMR structure of conserved eukaryotic protein ZK652.3 from C. elegans: a ubiquitin-li
NMR structure of conserved eukaryotic protein ZK652.3 from C. elegans: a ubiquitin-like fold.
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Proteins. 2002 Sep 1;48(4):733-6
Authors: Cort JR, Chiang Y, Zheng D, Montelione GT, Kennedy MA
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[NMR paper] Transforming growth factor beta 1: NMR signal assignments of the recombinant protein
Transforming growth factor beta 1: NMR signal assignments of the recombinant protein expressed and isotopically enriched using Chinese hamster ovary cells.
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Biochemistry. 1993 Feb 2;32(4):1152-63
Authors: Archer SJ, Bax A, Roberts AB, Sporn MB, Ogawa Y, Piez KA, Weatherbee JA, Tsang ML, Lucas R, Zheng BL
The transforming growth factor beta s are a homologous family of...
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08-21-2010 11:53 PM
[NMR paper] 1H-NMR studies of bovine platelet factor 4: histidine assignments and interactions wi
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Related Articles 1H-NMR studies of bovine platelet factor 4: histidine assignments and interactions with heparin.
Biochim Biophys Acta. 1991 Jun 24;1078(2):208-18
Authors: Talpas CJ, Walz DA, Lee L
1H-NMR spectroscopy has been used to assign and to characterize the two histidine C2H resonances of the heparin binding protein, bovine platelet factor 4. One histidine has a pKa value of 6.51 at 27 degrees C; the second histidine exhibits 2 pKa values...
NMR assignments of actin depolymerizing factor (ADF) like UNC-60A and cofilin like UNC-60B proteins of Caenorhabditis elegans.
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