NMR paper NMR analyses on the interactions of the yeast Tim50 C-terminal region with the presequence and Tim50 core domain.

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[NMR paper] NMR analyses on the interactions of the yeast Tim50 C-terminal region with the presequence and Tim50 core domain.

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01-28-2014, 11:53 AM

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NMR analyses on the interactions of the yeast Tim50 C-terminal region with the presequence and Tim50 core domain.

NMR analyses on the interactions of the yeast Tim50 C-terminal region with the presequence and Tim50 core domain.

NMR analyses on the interactions of the yeast Tim50 C-terminal region with the presequence and Tim50 core domain.

FEBS Lett. 2014 Jan 23;

Authors: Rahman B, Kawano S, Yunoki-Esaki K, Anzai T, Endo T

Abstract
The mitochondrial targeting signal in the presequence of mitochondrial precursor proteins is recognized by Tom20 and subsequently by Tim50 in mitochondria. Yeast Tim50 contains two presequence binding sites in the conserved core domain and in the fungi-specific C-terminal presequence binding domain (PBD). We report the NMR analyses on interactions of sPBD, a shorter variant of PBD, with presequences. The presequence is recognized by sPBD in a similar manner to Tom20. sPBD can also bind to the core domain of Tim50 through the presequence binding region, which could promote transfer of the presequence from sPBD to the core domain in Tim50.
STRUCTURED SUMMARY OF PROTEIN INTERACTIONS:: Tim50 sPBDandTim50corebindbynuclear magnetic resonance(View interaction) pSu9NandTim50 sPBDbindbynuclear magnetic resonance(1,2,3,4,5) pSu9NanddTim20bindbynuclear magnetic resonance(View interaction).

PMID: 24462684 [PubMed - as supplied by publisher]

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