[Question from NMRWiki Q&A forum] Impact of deuteration on relaxation rates PROTEIN NMR
Impact of deuteration on relaxation rates PROTEIN NMR
DEAR NMR WIKIERS
IS THEIR ANY NMR EXPERMENT TO KNOW THE IMPACT OF DEUTERATION ON RELAXATION REATES OF C13,N15,H ALFA , H BETA OF DEURATED PROTEIN (RANDOMLY DEUTERTED) Regards
SRI
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06-21-2011 03:40 PM
Sequence correction of random coil chemical shifts: correlation between neighbor correction factors and changes in the Ramachandran distribution
Sequence correction of random coil chemical shifts: correlation between neighbor correction factors and changes in the Ramachandran distribution
Abstract Random coil chemical shifts are necessary for secondary chemical shift analysis, which is the main NMR method for identification of secondary structure in proteins. One of the largest challenges in the determination of random coil chemical shifts is accounting for the effect of neighboring residues. The contributions from the neighboring residues are typically removed by using neighbor correction factors determined based on each...
[NMR paper] Relating side-chain mobility in proteins to rotameric transitions: insights from mole
Relating side-chain mobility in proteins to rotameric transitions: insights from molecular dynamics simulations and NMR.
Related Articles Relating side-chain mobility in proteins to rotameric transitions: insights from molecular dynamics simulations and NMR.
J Biomol NMR. 2005 Jun;32(2):151-62
Authors: Hu H, Hermans J, Lee AL
The dynamic aspect of proteins is fundamental to understanding protein stability and function. One of the goals of NMR studies of side-chain dynamics in proteins is to relate spin relaxation rates to discrete...
nmrlearner
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11-25-2010 08:21 PM
[NMR paper] Factors determining the reliable description of global tumbling parameters in solutio
Factors determining the reliable description of global tumbling parameters in solution NMR.
Related Articles Factors determining the reliable description of global tumbling parameters in solution NMR.
J Biomol NMR. 2002 Nov;24(3):215-29
Authors: Pawley NH, Gans JD, Nicholson LK
An accurate description of global tumbling of a protein is essential for correct analysis and interpretation of internal dynamics and thermodynamics. The accurate fitting of global tumbling parameters is affected by the number of experimental relaxation data points...
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11-24-2010 08:58 PM
[Question from NMRWiki Q&A forum] Relating 13C and 1H chemical shifts for representation of 2D CSPs
Relating 13C and 1H chemical shifts for representation of 2D CSPs
Hi, our lab uses the following relationship for the calculation of normalized CSPs from 1H-15N HSQC spectra: CSPnorm = ((0.154*Nshift)^2) + Hshift^2)^0.5
Can anyone provide the equivalent multiplier for 13C/1H normalized CSP calculations? I've been looking on the BMRB site but haven't had any success so far.
Cheers.
nmrlearner
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10-05-2010 02:04 AM
Crystallographic and NMR evaluation of the impact of peptide binding to the second PD
Crystallographic and NMR evaluation of the impact of peptide binding to the second PDZ domain of PTP1E.
Crystallographic and NMR evaluation of the impact of peptide binding to the second PDZ domain of PTP1E.
Biochemistry. 2010 Sep 14;
Authors: Zhang J, Sapienza PJ, Ke H, Chang A, Hengel SR, Wang H, Phillips GN, Lee AL
PDZ (PSD95/Discs large/ZO-1) domains are ubiquitous protein interaction motifs found in scaffolding proteins involved in signal transduction. Despite the fact that many PDZs show a limited tendency to undergo structural change, the...
nmrlearner
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09-16-2010 02:59 PM
[NMR paper] NMR studies in experimental allergic encephalomyelitis: factors which contribute to T
NMR studies in experimental allergic encephalomyelitis: factors which contribute to T1 and T2 values.
Related Articles NMR studies in experimental allergic encephalomyelitis: factors which contribute to T1 and T2 values.
Magn Reson Med. 1990 Apr;14(1):1-11
Authors: Karlik SJ, Gilbert JJ, Wong C, Vandervoort MK, Noseworthy JH
To determine the factors which may alter NMR relaxation times in multiple sclerosis (MS) lesions we measured the proton T1 and T2, specific gravity (SG), and histology in the central nervous system (CNS; 13-19 levels per...
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