The fungal cell wall plays a critical role in regulating cellular integrity and communication, and serves as a frontline defense against stress. It is also a prime target for the development of antifungal agents. The cell wall is comprised of diverse polysaccharides and proteins and poses a challenging target for high-resolution structural characterization. Recently, the solid-state nuclear magnetic resonance (ssNMR) analysis of intact Aspergillus fumigatus cells has provided atomic-level...
Solid-state NMR molecular snapshots of Aspergillus fumigatus cell wall architecture during a conidial morphotype transition
Solid-state NMR molecular snapshots of Aspergillus fumigatus cell wall architecture during a conidial morphotype transition
Gaëlle LamonAlons LendsIsabel ValsecchiSarah Sze Wah WongVincent DuprèsFrank LafontJames TolchardChristine SchmittAdeline MalletAxelle GrélardEstelle MorvanErick J. DufourcBirgit HabensteinJ. Iñaki GuijarroVishukumar AimaniandaAntoine LoquetaUniv. Bordeaux, Centre National de la Recherche Scientifique (CNRS), Bordeaux Institut National Polytechnique, Chemistry and Biology of Membranes and Nanoobjects (CBMN), Unité Mixte de Recherche (UMR) 5248, Institut Européen de...
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02-05-2023 05:05 AM
[NMR paper] Probing Cell-Surface Interactions in Fungal Cell Walls by High-Resolution 1H-detected Solid-State NMR Spectroscopy
Probing Cell-Surface Interactions in Fungal Cell Walls by High-Resolution 1H-detected Solid-State NMR Spectroscopy
Solid-state NMR (ssNMR) spectroscopy facilitates the non-destructive characterization of structurally heterogeneous biomolecules in their native setting, e.g., comprising proteins, lipids and polysaccharides. Here we demonstrate the utility of high and ultra-high field 1H-detected fast MAS ssNMR spectroscopy, which exhibits increased sensitivity and spectral resolution to further elucidate the atomic-level composition and structural arrangement of the cell wall of...
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10-02-2022 07:47 AM
[NMR paper] Solid-state NMR of plant and fungal cell walls: A critical review.
Solid-state NMR of plant and fungal cell walls: A critical review.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Solid-state NMR of plant and fungal cell walls: A critical review.
Solid State Nucl Magn Reson. 2020 Mar 26;107:101660
Authors: Zhao W, Fernando LD, Kirui A, Deligey F, Wang T
Abstract
The cell walls of plants and microbes are a central source for bio-renewable energy and the major targets of antibiotics and antifungal...
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04-07-2020 07:35 PM
Multidimensional solid-state NMR spectroscopy of plant cell walls #DNPNMR
From The DNP-NMR Blog:
Multidimensional solid-state NMR spectroscopy of plant cell walls #DNPNMR
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Wang, T., P. Phyo, and M. Hong, Multidimensional solid-state NMR spectroscopy of plant cell walls. Solid State Nuclear Magnetic Resonance, 2016. 78: p. 56-63.
http://www.sciencedirect.com/science/article/pii/S0926204016300595
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11-19-2016 08:35 PM
[NMR paper] Multidimensional solid-state NMR spectroscopy of plant cell walls.
Multidimensional solid-state NMR spectroscopy of plant cell walls.
Multidimensional solid-state NMR spectroscopy of plant cell walls.
Solid State Nucl Magn Reson. 2016 Aug 13;78:56-63
Authors: Wang T, Phyo P, Hong M
Abstract
Plant biomass has become an important source of bio-renewable energy in modern society. The molecular structure of plant cell walls is difficult to characterize by most atomic-resolution techniques due to the insoluble and disordered nature of the cell wall. Solid-state NMR (SSNMR) spectroscopy is uniquely...
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08-24-2016 04:39 PM
[NMR paper] Cellulose Structural Polymorphism in Plant Primary Cell Walls Investigated by High-Field 2D Solid-State NMR Spectroscopy and Density Functional Theory Calculations.
Cellulose Structural Polymorphism in Plant Primary Cell Walls Investigated by High-Field 2D Solid-State NMR Spectroscopy and Density Functional Theory Calculations.
Cellulose Structural Polymorphism in Plant Primary Cell Walls Investigated by High-Field 2D Solid-State NMR Spectroscopy and Density Functional Theory Calculations.
Biomacromolecules. 2016 May 18;
Authors: Wang T, Yang H, Kubicki JD, Hong M
Abstract
The native cellulose of bacterial, algal, and animal origins has been well studied structurally using X-ray and neutron...
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05-19-2016 10:13 AM
Sensitivity-enhanced solid-state NMR detection of expansin's target in plant cell walls
From The DNP-NMR Blog:
Sensitivity-enhanced solid-state NMR detection of expansin's target in plant cell walls
Wang, T., et al., Sensitivity-enhanced solid-state NMR detection of expansin's target in plant cell walls. Proc Natl Acad Sci U S A, 2013. 110(41): p. 16444-9.
http://www.ncbi.nlm.nih.gov/pubmed/24065828
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11-26-2013 01:19 AM
[NMR paper] Sensitivity-enhanced solid-state NMR detection of expansin's target in plant cell walls.
Sensitivity-enhanced solid-state NMR detection of expansin's target in plant cell walls.
Related Articles Sensitivity-enhanced solid-state NMR detection of expansin's target in plant cell walls.
Proc Natl Acad Sci U S A. 2013 Sep 24;
Authors: Wang T, Park YB, Caporini MA, Rosay M, Zhong L, Cosgrove DJ, Hong M
Abstract
Structure determination of protein binding to noncrystalline macromolecular assemblies such as plant cell walls (CWs) poses a significant structural biology challenge. CWs are loosened during growth by expansin proteins,...
New Vision of Cell Walls in Aspergillus fumigatus from Solid-State NMR Spectroscopy
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