Related ArticlesNew applications of solid-state NMR in structural biology.
Emerg Top Life Sci. 2018 Apr 20;2(1):57-67
Authors: van der Wel PCA
Abstract
Various recent developments in solid-state nuclear magnetic resonance (ssNMR) spectroscopy have enabled an array of new insights regarding the structure, dynamics, and interactions of biomolecules. In the ever more integrated world of structural biology, ssNMR studies provide structural and dynamic information that is complementary to the data accessible by other means. ssNMR enables the study of samples lacking a crystalline lattice, featuring static as well as dynamic disorder, and does so independent of higher-order symmetry. The present study surveys recent applications of biomolecular ssNMR and examines how this technique is increasingly integrated with other structural biology techniques, such as (cryo) electron microscopy, solution-state NMR, and X-ray crystallography. Traditional ssNMR targets include lipid bilayer membranes and membrane proteins in a lipid bilayer environment. Another classic application has been in the area of protein misfolding and aggregation disorders, where ssNMR has provided essential structural data on oligomers and amyloid fibril aggregates. More recently, the application of ssNMR has expanded to a growing array of biological assemblies, ranging from non-amyloid protein aggregates, protein-protein complexes, viral capsids, and many others. Across these areas, multidimensional magic angle spinning (MAS) ssNMR has, in the last decade, revealed three-dimensional structures, including many that had been inaccessible by other structural biology techniques. Equally important insights in structural and molecular biology derive from the ability of MAS ssNMR to probe information beyond comprehensive protein structures, such as dynamics, solvent exposure, protein-protein interfaces, and substrate-enzyme interactions.
Unique Insights into the Structural and Functional Biology of Membrane Proteins from Solid State NMR Spectroscopy
Unique Insights into the Structural and Functional Biology of Membrane Proteins from Solid State NMR Spectroscopy
Publication date: 2 February 2018
Source:Biophysical Journal, Volume 114, Issue 3, Supplement 1</br>
Author(s): Timothy Cross, Joana Paulino, Huajun Qin, Yiseul Shin, Cristian Escobar, Rongfu Zhang, Joshua Taylor, Yimin Miao, Riqiang Fu, Eduard Chekmenev, Ivan Hung, Zhehong Gan, Petr Gor'kov</br>
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02-07-2018 03:41 PM
[NMR paper] Beyond Structural Biology to Functional Biology: Solid-State NMR Experiments and Strategies for Understanding the M2 Proton Channel Conductance.
Beyond Structural Biology to Functional Biology: Solid-State NMR Experiments and Strategies for Understanding the M2 Proton Channel Conductance.
Related Articles Beyond Structural Biology to Functional Biology: Solid-State NMR Experiments and Strategies for Understanding the M2 Proton Channel Conductance.
J Phys Chem B. 2017 Apr 20;:
Authors: Qin H, Miao Y, Cross TA, Fu R
Abstract
In terms of structural biology, solid-state NMR experiments and strategies have been well established for resonance assignments leading to the...
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04-21-2017 03:35 PM
Structural biology applications of solid state MAS DNP NMR #DNPNMR
From The DNP-NMR Blog:
Structural biology applications of solid state MAS DNP NMR #DNPNMR
Akbey, U. and H. Oschkinat, Structural biology applications of solid state MAS DNP NMR. J Magn Reson, 2016. 269: p. 213-24.
http://www.ncbi.nlm.nih.gov/pubmed/27095695
nmrlearner
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08-31-2016 02:34 PM
[NMR paper] Solid-state NMR: An emerging technique in structural biology of self-assemblies.
Solid-state NMR: An emerging technique in structural biology of self-assemblies.
Related Articles Solid-state NMR: An emerging technique in structural biology of self-assemblies.
Biophys Chem. 2015 Jul 16;
Authors: Habenstein B, Loquet A
Abstract
Protein self-assemblies are ubiquitous biological systems involved in many cellular processes, ranging from bacterial and viral infection to the propagation of neurodegenerative disorders. Studying the atomic three-dimensional structures of protein self-assemblies is a particularly...
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08-04-2015 03:00 PM
Solid-state NMR enhanced by dynamic nuclear polarization as a novel tool for ribosome structural biology
From The DNP-NMR Blog:
Solid-state NMR enhanced by dynamic nuclear polarization as a novel tool for ribosome structural biology
Gelis, I., et al., Solid-state NMR enhanced by dynamic nuclear polarization as a novel tool for ribosome structural biology. J. Biomol. NMR, 2013. 56(2): p. 85-93.
http://dx.doi.org/10.1007/s10858-013-9721-2
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09-09-2013 05:14 PM
Structural topology of phospholamban pentamer in lipid bilayers by a hybrid solution and solid-state NMR method [Biophysics and Computational Biology]
Structural topology of phospholamban pentamer in lipid bilayers by a hybrid solution and solid-state NMR method
Verardi, R., Shi, L., Traaseth, N. J., Walsh, N., Veglia, G....
Date: 2011-05-31
Phospholamban (PLN) is a type II membrane protein that inhibits the sarcoplasmic reticulum Ca2+-ATPase (SERCA), thereby regulating calcium homeostasis in cardiac muscle. In membranes, PLN forms pentamers that have been proposed to function either as a storage for active monomers or as ion channels. Here, we report the T-state structure of pentameric PLN solved by a hybrid solution and...
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05-31-2011 11:41 PM
[NMR paper] Biomolecular solid state NMR: advances in structural methodology and applications to
Biomolecular solid state NMR: advances in structural methodology and applications to peptide and protein fibrils.
Related Articles Biomolecular solid state NMR: advances in structural methodology and applications to peptide and protein fibrils.
Annu Rev Phys Chem. 2001;52:575-606
Authors: Tycko R
Solid state nuclear magnetic resonance (NMR) methods can provide atomic-level structural constraints on peptides and proteins in forms that are not amenable to characterization by other high-resolution structural techniques, owing to insolubility,...
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11-19-2010 08:32 PM
Structural Biology- Practical NMR Applications-Quincy Teng
Structural Biology-Practical NMR Applications-Quincy Teng
I have just seen this book and have had time to skim it, seems like an excellent book to base an upper level undergrad or a grad course on, much easier to read than Cavanaugh and includes questions as well as learning goals for each section.
Has anyone else had a chance to go through this book?
New applications of solid-state NMR in structural biology.
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