[NMR paper] Natural compounds against Alzheimer's disease: molecular recognition of A?1-42 peptide by Salvia sclareoides extract and its major component, rosmarinic acid, as investigated by NMR.
Natural compounds against Alzheimer's disease: molecular recognition of A?1-42 peptide by Salvia sclareoides extract and its major component, rosmarinic acid, as investigated by NMR.
Related ArticlesNatural compounds against Alzheimer's disease: molecular recognition of A?1-42 peptide by Salvia sclareoides extract and its major component, rosmarinic acid, as investigated by NMR.
Chem Asian J. 2013 Mar;8(3):596-602
Authors: Airoldi C, Sironi E, Dias C, Marcelo F, Martins A, Rauter AP, Nicotra F, Jimenez-Barbero J
Abstract
Amyloid peptides, A?1-40 and A?1-42, represent major molecular targets to develop potential drugs and diagnostic tools for Alzheimer's Disease (AD). In fact, oligomeric and fibrillar aggregates generated by these peptides are amongst the principal components of amyloid plaques found post mortem in patients suffering from AD. Rosmarinic acid has been demonstrated to be effective in preventing the aggregation of amyloid peptides in vitro and to delay the progression of the disease in animal models. Nevertheless, no information is available about its molecular mechanism of action. Herein, we report the NMR characterization of the interaction of Salvia sclareoides extract and that of its major component, rosmarinic acid, with A?1-42 peptide, whose oligomers have been described as the most toxic A? species in vivo. Our data shed light on the structural determinants of rosmarinic acid-A?1-42 oligomers interaction, thus allowing the elucidation of its mechanism of action. They also provide important information for the rational design of new compounds with higher affinity for A? peptides to generate new anti-amyloidogenic molecules and/or molecular tools for the specific targeting of amyloid aggregates in vivo. In addition, we identified methyl caffeate, another natural compound present in different plants and human diet, as a good ligand of A?1-42 oligomers, which also shows anti-amyloidogenic activity. Finally, we demonstrated the possibility to exploit STD-NMR and trNOESY experiments to screen extracts from natural sources for the presence of A? peptide ligands.
Cryogenic solid state NMR studies of fibrils of the Alzheimer's disease amyloid-beta peptide: perspectives for DNP
From The DNP-NMR Blog:
Cryogenic solid state NMR studies of fibrils of the Alzheimer's disease amyloid-beta peptide: perspectives for DNP
Lopez del Amo, J.-M., et al., Cryogenic solid state NMR studies of fibrils of the Alzheimer’s disease amyloid-? peptide: perspectives for DNP. J. Biomol. NMR, 2013: p. 1-5.
http://www.ncbi.nlm.nih.gov/pubmed/23793606
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07-19-2013 09:20 PM
[NMR paper] Structural characterization by NMR of a double phosphorylated chimeric Peptide vaccine for treatment of Alzheimer's disease.
Structural characterization by NMR of a double phosphorylated chimeric Peptide vaccine for treatment of Alzheimer's disease.
Structural characterization by NMR of a double phosphorylated chimeric Peptide vaccine for treatment of Alzheimer's disease.
Molecules. 2013;18(5):4929-41
Authors: Ramírez-Gualito K, Richter M, Matzapetakis M, Singer D, Berger S
Abstract
Rational design of peptide vaccines becomes important for the treatment of some diseases such as Alzheimer's disease (AD) and related disorders. In this study, as part of a larger...
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04-30-2013 10:21 PM
[NMR paper] Structural analysis of the pyroglutamate-modified isoform of the Alzheimer's disease-related amyloid-? using NMR spectroscopy.
Structural analysis of the pyroglutamate-modified isoform of the Alzheimer's disease-related amyloid-? using NMR spectroscopy.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-2250-98-WileyOnlineLibrary-Button_120x27px_FullText.gif Related Articles Structural analysis of the pyroglutamate-modified isoform of the Alzheimer's disease-related amyloid-? using NMR spectroscopy.
J Pept Sci. 2012 Nov;18(11):691-5
Authors: Sun N, Hartmann R, Lecher J, Stoldt M, Funke SA, Gremer L, Ludwig HH, Demuth HU, Kleinschmidt M,...
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04-23-2013 08:37 PM
[NMR paper] NMR-based metabolomics of urine in a mouse model of Alzheimer's disease: identification of oxidative stress biomarkers.
NMR-based metabolomics of urine in a mouse model of Alzheimer's disease: identification of oxidative stress biomarkers.
Related Articles NMR-based metabolomics of urine in a mouse model of Alzheimer's disease: identification of oxidative stress biomarkers.
J Clin Biochem Nutr. 2013 Mar;52(2):133-8
Authors: Fukuhara K, Ohno A, Ota Y, Senoo Y, Maekawa K, Okuda H, Kurihara M, Okuno A, Niida S, Saito Y, Takikawa O
Abstract
Alzheimer's disease (AD) is the most common cause of neurodegenerative dementia among elderly patients. A biomarker...
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03-26-2013 01:30 PM
Alzheimer's analysed: New clues in brain disease
Alzheimer's analysed: New clues in brain disease
http://www.spectroscopynow.com/common/images/thumbnails/13af4d7f690.jpgRecent studies have added weight to the idea that amyloid-beta is a causative agent in Alzheimer's disease rather than the plaques formed from these chunks of misfolded peptide that deposit in the diseased brain. Now, European researchers have used REAPDOR solid-state NMR spectroscopy to analyse the hydrogen bonds in the tiny fibrils of amyloid-beta peptide, offering another perspective on this neurodegenerative disorder.
Source: Spectroscopynow.com
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02-03-2013 08:49 AM
[NMR paper] Holistic Control of Herbal Teas and Tinctures Based on Sage (Salvia officinalis L.) for Compounds with Beneficial and Adverse Effects using NMR Spectroscopy.
From Mendeley Biomolecular NMR group:
Holistic Control of Herbal Teas and Tinctures Based on Sage (Salvia officinalis L.) for Compounds with Beneficial and Adverse Effects using NMR Spectroscopy.
Analytical chemistry insights (2012). Pages: 1-12. Stephan G Walch, Dirk W Lachenmeier, Thomas Kuballa, Wolf Stühlinger, Yulia B Monakhova et al.
A methodology that utilizes (1)H-NMR spectroscopy has been developed to simultaneously analyze toxic terpenes (thujone and camphor), major polyphenolic compounds, the total antioxidant capacity (ORAC) and the Folin-Ciocalteu (FC) index in foods...
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08-24-2012 08:01 PM
[U. of Ottawa NMR Facility Blog] The Major Constituents of Natural Gas
The Major Constituents of Natural Gas
The major constituent of natural gas is methane however, other gaseous hydrocarbons are also present. One way to identify other components is to dissolve some natural gas in a solvent and examine the 1H NMR spectrum. The spectrum in the figure below was acquired on a sample prepared by bubbling natural gas through benzene-d6 for several minutes. The spectrum clearly shows the presence of methane, ethane, propane and water.http://1.bp.blogspot.com/-q-r6TSTHCdk/T3RrTY7dVUI/AAAAAAAAA8A/lYTb4acZIQU/s400/natural_gas.jpg The spectrum also indicated other...
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03-29-2012 10:11 PM
[NMR paper] Alzheimer's disease: NMR studies of asialo (GM1) and trisialo (GT1b) ganglioside inte
Alzheimer's disease: NMR studies of asialo (GM1) and trisialo (GT1b) ganglioside interactions with Abeta(1-40) peptide in a membrane mimic environment.
Related Articles Alzheimer's disease: NMR studies of asialo (GM1) and trisialo (GT1b) ganglioside interactions with Abeta(1-40) peptide in a membrane mimic environment.
Neurochem Res. 2004 Feb;29(2):447-53
Authors: Mandal PK, Pettegrew JW
Amyloid peptide (Abeta) is the major protein constituent of neuritic plaques in Alzheimer's disease (AD). This peptide is an amphipathic molecule that...