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Side-chains:
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Structure from NMR restraints:
Ab initio:
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Fragment-based:
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Template-based:
GeNMR
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WeNMR CS-Rosetta
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Homology-based:
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Torsion angles from chemical shifts:
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Secondary structure from chemical shifts:
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Flexibility from chemical shifts:
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Chemical shifts re-referencing:
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From structure:
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From sequence:
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Disordered proteins:
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Format conversion & validation:
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Protein disorder:
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Protein solubility:
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Solid-state NMR:
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Old 02-03-2013, 10:19 AM
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Default Native mass spectrometry of photosynthetic pigment-protein complexes.

Native mass spectrometry of photosynthetic pigment-protein complexes.

Related Articles Native mass spectrometry of photosynthetic pigment-protein complexes.

FEBS Lett. 2013 Jan 18;

Authors: Zhang H, Cui W, Gross ML, Blankenship RE

Abstract
Native mass spectrometry (MS), or as is sometimes called "native electrospray ionization" allows proteins in their native or near-native states in solution to be introduced into the gas phase and interrogated by mass spectrometry. This approach is now a powerful tool to investigate protein complexes. This article reviews the background of native MS of protein complexes and describes its strengths, taking photosynthetic pigment-protein complexes as examples. Native MS can be utilized in combination with other MS-based approaches to obtain complementary information to that provided by tools such as X-ray crystallography and NMR spectroscopy to understand the structure-function relationships of protein complexes. When additional information beyond that provided by native MS is required, other MS-based strategies can be successfully applied to augment the results of native MS.


PMID: 23337874 [PubMed - as supplied by publisher]



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