Related ArticlesNASR, An Effective Approach for Simultaneous Noise and Artifact Suppression in NMR Spectroscopy.
Anal Chem. 2013 Jan 22;
Authors: Jiang B, Luo F, Ding Y, Sun P, Zhang X, Jiang L, Li C, Mao XA, Yang D, Tang C, Liu M
Abstract
As a powerful tool for biological analysis, especially protein structure and dynamic studies, nuclear magnetic resonance (NMR) spectroscopy suffers from intrinsic low signal to nose ratio (SNR) and long acquisition time required for multidimensional (nD) experiments. Non-uniform sampling (NUS) can effectively speed up the experiment, but often introduces artifacts into spectrum. In addition to development of high sensitive hardwares and NMR pulse sequences, data post-processing is a relative simple and cost-effective method to improve the SNR and suppress the artifacts. In this work, we proposed an effective approach for simultaneously suppressing noise and artifacts based on the resampling principle The method is named NASR for short and tested using one-, two- and three-dimensional (1D, 2D & 3D) NMR spectra that were acquired using ether conventional or NUS (spiral and random, for 3D) approaches. The results reveal that the NASR is fast and applicable for improving the quality of 1D to nD NMR spectra with all kind of sampling schemes.
PMID: 23339579 [PubMed - as supplied by publisher]
Singular spectrum analysis for an automated solvent artifact removal and baseline correction of 1D NMR spectra.
Singular spectrum analysis for an automated solvent artifact removal and baseline correction of 1D NMR spectra.
Singular spectrum analysis for an automated solvent artifact removal and baseline correction of 1D NMR spectra.
J Magn Reson. 2011 Mar 6;
Authors: De Sanctis S, Malloni WM, Kremer W, Tomé AM, Lang EW, Neidig KP, Kalbitzer HR
NMR spectroscopy in biology and medicine is generally performed in aqueous solutions, thus in (1)H NMR spectroscopy, the dominant signal often stems from the partly suppressed solvent and can be many orders of...
nmrlearner
Journal club
0
04-05-2011 10:22 PM
Simultaneous convection compensation and solvent suppression in biomolecular NMR diffusion experiments
Simultaneous convection compensation and solvent suppression in biomolecular NMR diffusion experiments
Abstract Thermal convection and high intensity solvent resonances can significantly hamper diffusion estimates in pulsed gradient spin-echo nuclear magnetic resonance diffusion experiments on biomolecule samples. To overcome these two problems, a new double functional NMR diffusion sequence, double echo PGSTE-WATERGATE, is presented. The new sequence provides excellent convection compensation and solvent suppression (with a suppression factor in excess of at least 105 in a single scan)...
nmrlearner
Journal club
0
01-09-2011 12:46 PM
[NMR paper] Effective rotational correlation times of proteins from NMR relaxation interference.
Effective rotational correlation times of proteins from NMR relaxation interference.
Related Articles Effective rotational correlation times of proteins from NMR relaxation interference.
J Magn Reson. 2006 Jan;178(1):72-6
Authors: Lee D, Hilty C, Wider G, Wüthrich K
Knowledge of the effective rotational correlation times, tauc, for the modulation of anisotropic spin-spin interactions in macromolecules subject to Brownian motion in solution is of key interest for the practice of NMR spectroscopy in structural biology. The value of tauc enables...
nmrlearner
Journal club
0
12-01-2010 06:56 PM
[NMR paper] A simple and effective NMR cell for studies of encapsulated proteins dissolved in low
A simple and effective NMR cell for studies of encapsulated proteins dissolved in low viscosity solvents.
Related Articles A simple and effective NMR cell for studies of encapsulated proteins dissolved in low viscosity solvents.
J Biomol NMR. 2002 Aug;23(4):311-6
Authors: Flynn PF, Milton MJ, Babu CR, Wand AJ
Application of triple-resonance and isotope-edited-NOE methods to the study of increasingly larger macromolecules and their complexes remains a central goal of solution NMR spectroscopy. The slow reorientational motion of larger molecules...
nmrlearner
Journal club
0
11-24-2010 08:58 PM
[Question from NMRWiki Q&A forum] How to fix the broad antiphase artifact after SOL function in nmrPipe?
How to fix the broad antiphase artifact after SOL function in nmrPipe?
Hi, we've tried to apply SOL function of nmrPipe to process a 3D 13C edited NOESY. and the result is a wide antiphase-looking peak on water resonance that strongly distorts the baseline.
Would you have any idea what might be causing this? First image is a screenshot of data using SOL processing step and the second one was prepared without SOL.
Thanks.
http://qa.nmrwiki.org/upfiles/12853514685812478.png
nmrlearner
News from other NMR forums
0
09-24-2010 07:36 PM
[Stan NMR blog] Noise Figure and Equivalent Input Noise
Noise Figure and Equivalent Input Noise
An educational article about the definitions of - and the relation between - the two concepts.
More...
nmrlearner
News from NMR blogs
0
08-21-2010 06:14 PM
Automated solvent artifact removal and base plane correction of multidimensional NMR
Abstract Strong solvent signals lead to a disappearance of weak protein signals close to the solvent resonance frequency and to base plane variations all over the spectrum. AUREMOL-SSA provides an automated approach for solvent artifact removal from multidimensional NMR protein spectra. Its core algorithm is based on singular spectrum analysis (SSA) in the time domain and is combined with an automated base plane correction in the frequency domain. The performance of the method has been tested on synthetic and experimental spectra including two-dimensional NOESY and TOCSY spectra and a...
NASR, An Effective Approach for Simultaneous Noise and Artifact Suppression in NMR Spectroscopy.
Linear Mode
