Nanodiscs vs. Macrodiscs for NMR of Membrane Proteins.

		BioNMR > Educational resources > Journal club
Nanodiscs vs. Macrodiscs for NMR of Membrane Proteins.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

09-23-2011, 05:30 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Nanodiscs vs. Macrodiscs for NMR of Membrane Proteins.

Nanodiscs vs. Macrodiscs for NMR of Membrane Proteins.

Nanodiscs vs. Macrodiscs for NMR of Membrane Proteins.

Biochemistry. 2011 Sep 21;

Authors: Park SH, Berkamp S, Cook GA, Chan MK, Viadiu H, Opella SJ

Abstract
It is challenging to find membrane mimics that stabilize the native structure, dynamics, and functions of membrane proteins. In a recent advance, nanodiscs have been shown to provide a bilayer environment compatible with solution NMR. Increasing the lipid to "belt" peptide ratio expands their diameter, slows their reorientation rate, and enables the protein-containing discs to be aligned in a magnetic field for Oriented Sample solid-state NMR. Here, we compare the spectroscopic properties of membrane proteins with between one and seven trans-membrane helices in q=0.1 isotropic bicelles, ~10 nm diameter isotropic nanodiscs, ~30 nm diameter magnetically aligned macrodiscs, and q=5 bicelles.

PMID: 21936505 [PubMed - as supplied by publisher]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Nanodiscs versus Macrodiscs for NMR of Membrane Proteins Nanodiscs versus Macrodiscs for NMR of Membrane Proteins http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/bi201289c/aop/images/medium/bi-2011-01289c_0002.gif Biochemistry DOI: 10.1021/bi201289c http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/bichaw/~4/4XXVT7tcC8w More...	nmrlearner	Journal club	0	09-30-2011 08:01 PM
Preparation of a functional GABARAP-lipid conjugate in nanodiscs and its investigation by solution NMR spectroscopy. Preparation of a functional GABARAP-lipid conjugate in nanodiscs and its investigation by solution NMR spectroscopy. Preparation of a functional GABARAP-lipid conjugate in nanodiscs and its investigation by solution NMR spectroscopy. Chembiochem. 2010 Sep 24;11(14):1967-70 Authors: Ma P, Mohrlüder J, Schwarten M, Stoldt M, Singh SK, Hartmann R, Pacheco V, Willbold D	nmrlearner	Journal club	0	01-21-2011 01:22 AM
Postdoc Membrane Proteins Postdoc Membrane Proteins Structural studies of membrane proteins by NMR A post-doctoral position is available to work on the structural studies of a variety of membrane proteins (cytochrome P450, cytochrome b5, cytochrome-P450-reductase, and a combination of these proteins to probe protein-protein interactions) and amyloid proteins using NMR spectroscopy. We are presently utilizing a combination of high-resolution solution NMR and solid-state (static and MAS) NMR techniques to solve high-resolution structures and characterize the dynamics of membrane proteins embedded in suitable...	nmrlearner	Job marketplace	0	01-12-2011 01:45 PM
[NMR paper] How to prepare membrane proteins for solid-state NMR: A case study on the alpha-helical integral membrane protein diacylglycerol kinase from E. coli. How to prepare membrane proteins for solid-state NMR: A case study on the alpha-helical integral membrane protein diacylglycerol kinase from E. coli. Related Articles How to prepare membrane proteins for solid-state NMR: A case study on the alpha-helical integral membrane protein diacylglycerol kinase from E. coli. Chembiochem. 2005 Sep;6(9):1693-700 Authors: Lorch M, Faham S, Kaiser C, Weber I, Mason AJ, Bowie JU, Glaubitz C Several studies have demonstrated that it is viable to use microcrystalline preparations of water-soluble proteins as...	nmrlearner	Journal club	0	12-01-2010 06:56 PM
[NMR paper] Techniques and applications of NMR to membrane proteins. Techniques and applications of NMR to membrane proteins. Related Articles Techniques and applications of NMR to membrane proteins. Mol Membr Biol. 2004 May-Jun;21(3):129-41 Authors: Nielsen N, Malmendal A, Vosegaard T The fact that membrane proteins are notoriously difficult to analyse using standard protocols for atomic-resolution structure determination methods have motivated adaptation of these techniques to membrane protein studies as well as development of new technologies. With this motivation, liquid-state nuclear magnetic resonance...	nmrlearner	Journal club	0	11-24-2010 09:51 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off