Nanodiscs vs. Macrodiscs for NMR of Membrane Proteins.
Nanodiscs vs. Macrodiscs for NMR of Membrane Proteins.
Nanodiscs vs. Macrodiscs for NMR of Membrane Proteins.
Biochemistry. 2011 Sep 21;
Authors: Park SH, Berkamp S, Cook GA, Chan MK, Viadiu H, Opella SJ
Abstract
It is challenging to find membrane mimics that stabilize the native structure, dynamics, and functions of membrane proteins. In a recent advance, nanodiscs have been shown to provide a bilayer environment compatible with solution NMR. Increasing the lipid to "belt" peptide ratio expands their diameter, slows their reorientation...
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09-23-2011 05:30 PM
Preparation of a functional GABARAP-lipid conjugate in nanodiscs and its investigation by solution NMR spectroscopy.
Preparation of a functional GABARAP-lipid conjugate in nanodiscs and its investigation by solution NMR spectroscopy.
Preparation of a functional GABARAP-lipid conjugate in nanodiscs and its investigation by solution NMR spectroscopy.
Chembiochem. 2010 Sep 24;11(14):1967-70
Authors: Ma P, Mohrlüder J, Schwarten M, Stoldt M, Singh SK, Hartmann R, Pacheco V, Willbold D
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01-21-2011 01:22 AM
Postdoc Membrane Proteins
Postdoc Membrane Proteins
Structural studies of membrane proteins by NMR
A post-doctoral position is available to work on the structural studies of a variety of
membrane proteins (cytochrome P450, cytochrome b5, cytochrome-P450-reductase,
and a combination of these proteins to probe protein-protein interactions)
and amyloid proteins using NMR spectroscopy. We are presently utilizing a combination
of high-resolution solution NMR and solid-state (static and MAS) NMR techniques to
solve high-resolution structures and characterize the dynamics of membrane proteins
embedded in suitable...
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01-12-2011 01:45 PM
[NMR paper] How to prepare membrane proteins for solid-state NMR: A case study on the alpha-helical integral membrane protein diacylglycerol kinase from E. coli.
How to prepare membrane proteins for solid-state NMR: A case study on the alpha-helical integral membrane protein diacylglycerol kinase from E. coli.
Related Articles How to prepare membrane proteins for solid-state NMR: A case study on the alpha-helical integral membrane protein diacylglycerol kinase from E. coli.
Chembiochem. 2005 Sep;6(9):1693-700
Authors: Lorch M, Faham S, Kaiser C, Weber I, Mason AJ, Bowie JU, Glaubitz C
Several studies have demonstrated that it is viable to use microcrystalline preparations of water-soluble proteins as...
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12-01-2010 06:56 PM
[NMR paper] Lipid modifications of a Ras peptide exhibit altered packing and mobility versus host membrane as detected by 2H solid-state NMR.
Lipid modifications of a Ras peptide exhibit altered packing and mobility versus host membrane as detected by 2H solid-state NMR.
Related Articles Lipid modifications of a Ras peptide exhibit altered packing and mobility versus host membrane as detected by 2H solid-state NMR.
J Am Chem Soc. 2005 Sep 7;127(35):12263-72
Authors: Vogel A, Katzka CP, Waldmann H, Arnold K, Brown MF, Huster D
The human N-ras protein binds to cellular membranes by insertion of two covalently bound posttranslational lipid modifications, which is crucial for its...
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12-01-2010 06:56 PM
[NMR paper] Techniques and applications of NMR to membrane proteins.
Techniques and applications of NMR to membrane proteins.
Related Articles Techniques and applications of NMR to membrane proteins.
Mol Membr Biol. 2004 May-Jun;21(3):129-41
Authors: Nielsen N, Malmendal A, Vosegaard T
The fact that membrane proteins are notoriously difficult to analyse using standard protocols for atomic-resolution structure determination methods have motivated adaptation of these techniques to membrane protein studies as well as development of new technologies. With this motivation, liquid-state nuclear magnetic resonance...
Nanodiscs versus Macrodiscs for NMR of Membrane Proteins
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