NMR chemical shift changes can report on the functional dynamics of biomacromolecules in solution with sizes >1 MDa. However, their interpretation requires chemical shift assignments to individual nuclei, which for large molecules often can only be obtained by tedious point mutations that may interfere with function. We present here an efficient pseudocontact shift NMR method to assign biomacromolecules using bound antibodies tagged with lanthanoid DOTA chelators. The stability of the antibody...
[ASAP] Nanobody GPS by PCS: An Efficient New NMR Analysis Method for G Protein Coupled Receptors and Other Large Proteins
Nanobody GPS by PCS: An Efficient New NMR Analysis Method for G Protein Coupled Receptors and Other Large Proteins
Feng-Jie Wu, Pascal S. Rieder, Layara Akemi Abiko, Philip Ro?ßler, Alvar D. Gossert, Daniel Ha?ussinger, and Stephan Grzesiek
https://pubs.acs.org/cms/10.1021/jacs.2c09692/asset/images/medium/ja2c09692_0007.gif
Journal of the American Chemical Society
DOI: 10.1021/jacs.2c09692
[NMR paper] Magic angle spinning NMR of G protein-coupled receptors
Magic angle spinning NMR of G protein-coupled receptors
G protein-coupled receptors (GPCRs) have a simple seven transmembrane helix architecture which has evolved to recognize a diverse number of chemical signals. The more than 800 GPCRs encoded in the human genome function as receptors for vision, smell and taste, and mediate key physiological processes. Consequently, these receptors are a major target for pharmaceuticals. Protein crystallography and electron cryo-microscopy have provided high resolution structures of many GPCRs in both active and...
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03-18-2022 12:01 AM
When Heterotrimeric G Proteins Are Not Activated by G Protein-Coupled Receptors: Structural Insights and Evolutionary Conservation
When Heterotrimeric G Proteins Are Not Activated by G Protein-Coupled Receptors: Structural Insights and Evolutionary Conservation
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.7b00845/20171016/images/medium/bi-2017-00845a_0003.gif
Biochemistry
DOI: 10.1021/acs.biochem.7b00845
http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/bichaw/~4/4Eup-rPKLl8
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10-17-2017 10:19 AM
Global G-Protein Coupled Receptors (GPCRs) Industry - Sacramento Bee
Global G-Protein Coupled Receptors (GPCRs) Industry - Sacramento Bee
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Global G-Protein Coupled Receptors (GPCRs) Industry
Sacramento Bee
NMR Spectroscopy: An Invaluable Tool to Study GPCR Structures II-4 ... G-PROTEIN COUPLED RECEPTORS (GPCR) - A CONCEPTUAL OVERVIEW II-7GPCR: Gateways to Cell II-7Classification of GPCRs II-7Table 1: List of Identified Known and Orphan (Non-Sensory)GPCRs ...
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08-15-2012 08:54 AM
Global G-Protein Coupled Receptors (GPCRs) Industry - Sacramento Bee
Global G-Protein Coupled Receptors (GPCRs) Industry - Sacramento Bee
<img alt="" height="1" width="1" />
Global G-Protein Coupled Receptors (GPCRs) Industry
Sacramento Bee
This report analyzes the Global market for G-Protein Coupled Receptors (GPCRs) in ... NMR Spectroscopy: An Invaluable Tool to Study GPCR Structures II-4 ...
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nmrlearner
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06-13-2012 11:34 AM
Structure and function of G protein-coupled receptors using NMR spectroscopy
Structure and function of G protein-coupled receptors using NMR spectroscopy
Publication year: 2010
Source:Progress in Nuclear Magnetic Resonance Spectroscopy, Volume 57, Issue 2</br>
Joseph A. Goncalves, Shivani Ahuja, Sina Erfani, Markus Eilers, Steven O. Smith</br>
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nmrlearner
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03-09-2012 09:16 AM
Structure and function of G protein-coupled receptors using NMR spectroscopy
Structure and function of G protein-coupled receptors using NMR spectroscopy
Publication year: 2010
Source: Progress in Nuclear Magnetic Resonance Spectroscopy, In Press, Accepted Manuscript, Available online 12 May 2010</br>
Joseph A., Goncalves , Shivani, Ahuja , Sina, Erfani , Markus, Eilers , Steven O., Smith</br>
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