Rossukon Thongwichian, Jonas Kosten, Uwe Benary, Honor May Rose, Marchel Stuiver, Francois-Xavier Theillet, Alexander Dose, Birgit Koch, Hideki Yokoyama, Dirk Schwarzer, Jana Wolf and Philipp Selenko
Journal of the American Chemical Society
DOI: 10.1021/jacs.5b02987
Real-time pure shift 15 N HSQC of proteins: a real improvement in resolution and sensitivity
Real-time pure shift 15 N HSQC of proteins: a real improvement in resolution and sensitivity
Abstract
Spectral resolution in proton NMR spectroscopy is reduced by the splitting of resonances into multiplets due to the effect of homonuclear scalar couplings. Although these effects are often hidden in protein NMR spectroscopy by low digital resolution and routine apodization, behind the scenes homonuclear scalar couplings increase spectral overcrowding. The possibilities for biomolecular NMR offered by new pure shift NMR methods are illustrated here....
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03-04-2015 08:56 AM
[NMR paper] Real-time NMR monitoring of biological activities in complex physiological environments.
Real-time NMR monitoring of biological activities in complex physiological environments.
Real-time NMR monitoring of biological activities in complex physiological environments.
Curr Opin Struct Biol. 2015 Feb 26;32C:39-47
Authors: Smith MJ, Marshall CB, Theillet FX, Binolfi A, Selenko P, Ikura M
Abstract
Biological reactions occur in a highly organized spatiotemporal context and with kinetics that are modulated by multiple environmental factors. To integrate these variables in our experimental investigations of 'native'...
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03-03-2015 08:34 PM
NMR profiling of histone deacetylase and acetyl-transferase activities in real time.
NMR profiling of histone deacetylase and acetyl-transferase activities in real time.
NMR profiling of histone deacetylase and acetyl-transferase activities in real time.
ACS Chem Biol. 2011 May 20;6(5):419-24
Authors: Dose A, Liokatis S, Theillet FX, Selenko P, Schwarzer D
Abstract
Histone deacetylases (HDACs) and histone acetyl-transferases (HATs) are universal regulators of eukaryotic transcriptional activity and emerging therapeutic targets for human diseases. Here we describe the generation of isotope-labeled deacetylation and...
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09-07-2011 06:28 PM
Interfacial enzyme kinetics of a membrane bound kinase analyzed by real-time MAS-NMR.
Interfacial enzyme kinetics of a membrane bound kinase analyzed by real-time MAS-NMR.
Interfacial enzyme kinetics of a membrane bound kinase analyzed by real-time MAS-NMR.
Nat Chem Biol. 2011 Mar 20;
Authors: Ullrich SJ, Hellmich UA, Ullrich S, Glaubitz C
The simultaneous observation of interdependent reactions within different phases as catalyzed by membrane-bound enzymes is still a challenging task. One such enzyme, the Escherichia coli integral membrane protein diacylglycerol kinase (DGK), is a key player in lipid regulation. It catalyzes the...
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03-23-2011 05:41 PM
[NMR paper] Object-oriented approach to drug design enabled by NMR SOLVE: first real-time structu
Object-oriented approach to drug design enabled by NMR SOLVE: first real-time structural tool for characterizing protein-ligand interactions.
Related Articles Object-oriented approach to drug design enabled by NMR SOLVE: first real-time structural tool for characterizing protein-ligand interactions.
J Cell Biochem Suppl. 2001;Suppl 37:99-105
Authors: Sem DS, Yu L, Coutts SM, Jack R
As a result of genomics efforts, the number of protein drug targets is expected to increase by an order of magnitude. Functional genomics efforts are identifying...
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11-19-2010 08:32 PM
[NMR paper] Folding kinetics of the SH3 domain of PI3 kinase by real-time NMR combined with optic
Folding kinetics of the SH3 domain of PI3 kinase by real-time NMR combined with optical spectroscopy.
Related Articles Folding kinetics of the SH3 domain of PI3 kinase by real-time NMR combined with optical spectroscopy.
J Mol Biol. 1998 Feb 27;276(3):657-67
Authors: Guijarro JI, Morton CJ, Plaxco KW, Campbell ID, Dobson CM
The refolding kinetics of the chemically denatured SH3 domain of phosphatidylinositol 3'-kinase (PI3-SH3) have been monitored by real-time one-dimensional 1H NMR coupled with a variety of other biophysical techniques. These...
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11-17-2010 11:06 PM
[NMR paper] Following protein folding in real time using NMR spectroscopy.
Following protein folding in real time using NMR spectroscopy.
Related Articles Following protein folding in real time using NMR spectroscopy.
Nat Struct Biol. 1995 Oct;2(10):865-70
Authors: Balbach J, Forge V, van Nuland NA, Winder SL, Hore PJ, Dobson CM
The refolding of apo bovine alpha-lactalbumin has been monitored in real time by NMR spectroscopy following rapid in situ dilution of a chemically denatured state. By examining individual resonances in the time-resolved NMR spectra, the native state has been shown to emerge in a cooperative...
A Multiplexed NMR-Reporter Approach to Measure Cellular Kinase and Phosphatase Activities in Real-Time
Linear Mode
