Multiple Fluorescence Labeling and Two Dimensional FTIR - 13C NMR Heterospectral Correlation Spectroscopy to Characterize Extracellular Polymeric Substances in Biofilms Produced during Composting.
Multiple Fluorescence Labeling and Two Dimensional FTIR - 13C NMR Heterospectral Correlation Spectroscopy to Characterize Extracellular Polymeric Substances in Biofilms Produced during Composting.
Multiple Fluorescence Labeling and Two Dimensional FTIR - 13C NMR Heterospectral Correlation Spectroscopy to Characterize Extracellular Polymeric Substances in Biofilms Produced during Composting.
Environ Sci Technol. 2011 Sep 13;
Authors: Yu G, Tang Z, Yangchun X, Shen Q
Abstract
Knowledge on the structure and function of extracellular polymeric substances (EPS) in biofilms is essential for understanding biodegradation processes. Herein, a novel method based on multiple fluorescence labeling and two-dimensional (2D) FTIR - 13C NMR heterospectral correlation spectroscopy was developed to gain insight on the composition, architecture, and function of EPS in biofilms during composting. Compared to other environmental biofilms, biofilms in the thermophilic (> 55oC) and cooling (mature) stage of composting have distinct characteristics. The results of multiple fluorescence labeling demonstrated that biofilms were distributed in clusters during the thermophilic stage (day 14), and dead cells were detected. In the mature stage (day 26), biofilm formed a continuous layer with a thickness of approximately 20-100 µm around the compost, and re-colonization of cells at the surface of the compost was easily observed. Through 2D FTIR - 13C NMR correlation heterospectral spectroscopy, the following trend in the ease of the degradation of organic compounds was observed: heteropolysaccharides > cellulose > amide I in proteins. And proteins and cellulose showed significantly more degradation than heteropolysaccharides. In summary, the combination of multiple fluorescence labeling and 2D correlation spectroscopy is a promising approach for the characterization of EPS in biofilms.
PMID: 21910500 [PubMed - as supplied by publisher]
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Multiple Fluorescence Labeling and Two Dimensional FTIR - 13C NMR Heterospectral Correlation Spectroscopy to Characterize Extracellular Polymeric Substances in Biofilms Produced during Composting.
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