RgNanH is an intramolecular trans-sialidase expressed by the human gut symbiont Ruminococcus gnavus, to utilise intestinal sialylated mucin glycan epitopes. Its catalytic domain, belonging to glycoside hydrolase GH33 family, cleaves off terminal sialic acid residues from mucins, releasing 2,7-anhydro-Neu5Ac which is then used as metabolic substrate by R. gnavus to proliferate in the mucosal environment. RgNanH is one of the three intramolecular trans-sialidases (IT-sialidases) characterised to...
[NMR paper] Derivatives of GdAAZTA Conjugated to Amino Acids: A Multinuclear and Multifrequency NMR Study
Derivatives of GdAAZTA Conjugated to Amino Acids: A Multinuclear and Multifrequency NMR Study
The GdAAZTA (AAZTA = 6-amino-6-methylperhydro-1,4-diazepinetetraacetic acid) complex represents a platform of great interest for the design of innovative MRI probes due to its remarkable magnetic properties, thermodynamic stability, kinetic inertness, and high chemical versatility. Here, we detail the synthesis and characterization of new derivatives functionalized with four amino acids with different molecular weights and charges: l-serine, l-cysteine, l-lysine, and l-glutamic acid. The main...
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08-11-2022 01:49 PM
[NMR paper] 19F-NMR Unveils the Ligand-Induced Conformation of a Catalytically Inactive Twisted Homodimer of tRNA-Guanine Transglycosylase
19F-NMR Unveils the Ligand-Induced Conformation of a Catalytically Inactive Twisted Homodimer of tRNA-Guanine Transglycosylase
Understanding the structural arrangements of protein oligomers can support the design of ligands that interfere with their function in order to develop new therapeutic concepts for disease treatment. Recent crystallographic studies have elucidated a novel twisted and functionally inactive form of the homodimeric enzyme tRNA-guanine transglycosylase (TGT), a putative target in the fight against shigellosis. Active-site ligands have been identified that stimulate the...
[NMR paper] The Transient Complex of Cytochrome c and Cytochrome c Peroxidase: Insights into the Encounter Complex from Multifrequency EPR and NMR Spectroscopy.
The Transient Complex of Cytochrome c and Cytochrome c Peroxidase: Insights into the Encounter Complex from Multifrequency EPR and NMR Spectroscopy.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-7388-69-wiley-full-text.png Related Articles The Transient Complex of Cytochrome c and Cytochrome c Peroxidase: Insights into the Encounter Complex from Multifrequency EPR and NMR Spectroscopy.
Chemphyschem. 2020 Apr 17;:
Authors: van Son M, Schilder JT, Di Savino A, Blok A, Ubbink M, Huber M
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04-20-2020 05:10 PM
Development of the Multifrequency Gyrotron FU CW GV with Gaussian Beam Output
From The DNP-NMR Blog:
Development of the Multifrequency Gyrotron FU CW GV with Gaussian Beam Output
Tatematsu, Y., et al., Development of the Multifrequency Gyrotron FU CW GV with Gaussian Beam Output. J Infrared Milli Terahz Waves, 2015: p. 1-12.
http://dx.doi.org/10.1007/s10762-015-0173-5
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News from NMR blogs
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06-19-2015 04:44 PM
NMR Thesis Defended: Vladimir Michaelis
NMR Thesis Defended: Vladimir Michaelis
Congratulations go to a long-time 900 Facility user Vladimir Michaelis who has just defended his Ph.D. Thesis! Well done, Vlad!
Vladimir Michaelis (Department of Chemistry, University of Manitoba) November 10, 2010
Supervisor: Prof. Scott Kroeker
Ph.D. thesis "Nuclear Magnetic Resonance Studies of Disorder and Local Structure in Borate and Germanate Materials"
Vlad and Scott just published an important paper on 73Ge solid-state NMR in the Journal of Physical Chemistry which was part of his thesis project
nmrlearner
NMR theses
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11-14-2010 05:16 AM
[NMR paper] Molecular dynamics-derived conformation and intramolecular interaction analysis of th
Molecular dynamics-derived conformation and intramolecular interaction analysis of the N-acetyl-9-O-acetylneuraminic acid-containing ganglioside GD1a and NMR-based analysis of its binding to a human polyclonal immunoglobulin G fraction with selectivity for O-acetylated sialic acids.
Related Articles Molecular dynamics-derived conformation and intramolecular interaction analysis of the N-acetyl-9-O-acetylneuraminic acid-containing ganglioside GD1a and NMR-based analysis of its binding to a human polyclonal immunoglobulin G fraction with selectivity for O-acetylated sialic acids.
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Multifrequency-STD NMR unveils the first Michaelis complex of an intramolecular trans-sialidase from Ruminococcus gnavus
Linear Mode
