Ultrafast magic angle spinning (MAS) technology and 1H detection have dramatically enhanced the sensitivity of solid-state NMR (ssNMR) spectroscopy of biopolymers. We previously showed that, when combined with polarization optimized experiments (POE), these advancements enable the simultaneous acquisition of multi-dimensional 1H- or 13C-detected experiments using a single receiver. Here, we propose a new sub-class within the POE family, namely HC-DUMAS, HC-MEIOSIS, and HC-MAeSTOSO, that utilize dual receiver technology for the simultaneous detection of 1H and 13C nuclei. We also expand this approach to record 1H-, 13C-, and 15N-detected homonuclear 2D spectra simultaneously using three independent receivers. The combination of POE and multi-receiver technology will further shorten the total experimental time of ssNMR experiments for biological solids.
[NMR paper] Experimental Aspects of Polarization Optimized Experiments (POE) for Magic Angle Spinning Solid-State NMR of Microcrystalline and Membrane-Bound Proteins.
Experimental Aspects of Polarization Optimized Experiments (POE) for Magic Angle Spinning Solid-State NMR of Microcrystalline and Membrane-Bound Proteins.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles Experimental Aspects of Polarization Optimized Experiments (POE) for Magic Angle Spinning Solid-State NMR of Microcrystalline and Membrane-Bound Proteins.
Methods Mol Biol. 2018;1688:37-53
Authors: Gopinath T, Veglia G
Abstract
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11-21-2017 10:10 PM
[NMR paper] Proton-Based Ultrafast Magic Angle Spinning Solid-State NMR Spectroscopy.
Proton-Based Ultrafast Magic Angle Spinning Solid-State NMR Spectroscopy.
Related Articles Proton-Based Ultrafast Magic Angle Spinning Solid-State NMR Spectroscopy.
Acc Chem Res. 2017 Mar 29;:
Authors: Zhang R, Mroue KH, Ramamoorthy A
Abstract
Protons are vastly abundant in a wide range of exciting macromolecules and thus can be a powerful probe to investigate the structure and dynamics at atomic resolution using solid-state NMR (ssNMR) spectroscopy. Unfortunately, the high signal sensitivity, afforded by the high...
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03-30-2017 06:42 PM
[NMR paper] Backbone assignment of perdeuterated proteins by solid-state NMR using proton detection and ultrafast magic-angle spinning.
Backbone assignment of perdeuterated proteins by solid-state NMR using proton detection and ultrafast magic-angle spinning.
Related Articles Backbone assignment of perdeuterated proteins by solid-state NMR using proton detection and ultrafast magic-angle spinning.
Nat Protoc. 2017 Apr;12(4):764-782
Authors: Fricke P, Chevelkov V, Zinke M, Giller K, Becker S, Lange A
Abstract
Solid-state NMR (ssNMR) is a technique that allows the study of protein structure and dynamics at atomic detail. In contrast to X-ray crystallography and...
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03-10-2017 04:23 PM
[NMR paper] A Novel High-Resolution and Sensitivity-Enhanced Three-Dimensional Solid-State NMR Experiment Under Ultrafast Magic Angle Spinning Conditions.
A Novel High-Resolution and Sensitivity-Enhanced Three-Dimensional Solid-State NMR Experiment Under Ultrafast Magic Angle Spinning Conditions.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.nature.com-images-lo_npg.gif http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.ncbi.nlm.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles A Novel High-Resolution and Sensitivity-Enhanced Three-Dimensional Solid-State NMR Experiment Under Ultrafast Magic Angle Spinning Conditions.
Sci Rep. 2015;5:11810
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08-03-2016 04:58 AM
Recovery of bulk proton magnetization and sensitivity enhancement in ultrafast magic-angle spinning solid-state NMR
From The DNP-NMR Blog:
Recovery of bulk proton magnetization and sensitivity enhancement in ultrafast magic-angle spinning solid-state NMR
A large portion of the magnetization in a CP experiment remains unused after an experiment and different strategies exist to make better use of the proton magnetization. Here the authors show their results of testing 7 different cp schemes. Although not directly related to DNP these techniques are still very valuable to increase the sensitivity of an NMR experiment especially in combination with DNP.
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03-30-2015 06:04 PM
[NMR paper] Multiple acquisition of magic angle spinning solid-state NMR experiments using one receiver: Application to microcrystalline and membrane protein preparations.
Multiple acquisition of magic angle spinning solid-state NMR experiments using one receiver: Application to microcrystalline and membrane protein preparations.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Multiple acquisition of magic angle spinning solid-state NMR experiments using one receiver: Application to microcrystalline and membrane protein preparations.
J Magn Reson. 2015 Apr;253:143-53
Authors: Gopinath T, Veglia G
Abstract
Solid-state NMR...
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03-24-2015 09:58 PM
Multiple acquisition of magic angle spinning solid-state NMR experiments using one receiver: Application to microcrystalline and membrane protein preparations
Multiple acquisition of magic angle spinning solid-state NMR experiments using one receiver: Application to microcrystalline and membrane protein preparations
Publication date: April 2015
Source:Journal of Magnetic Resonance, Volume 253</br>
Author(s): T. Gopinath , Gianluigi Veglia</br>
Solid-state NMR spectroscopy of proteins is a notoriously low-throughput technique. Relatively low-sensitivity and poor resolution of protein samples require long acquisition times for multidimensional NMR experiments. To speed up data acquisition, we developed a family of...
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03-20-2015 01:48 AM
[NMR paper] Experiments Optimized for Magic Angle Spinning and Oriented Sample Solid-State NMR of Proteins.
Experiments Optimized for Magic Angle Spinning and Oriented Sample Solid-State NMR of Proteins.
Related Articles Experiments Optimized for Magic Angle Spinning and Oriented Sample Solid-State NMR of Proteins.
J Phys Chem B. 2013 Sep 17;
Authors: Das BB, Lin EC, Opella SJ
Abstract
Structure determination by solid-state NMR of proteins is rapidly advancing as result of recent developments of samples, experimental methods, and calculations. There are a number of different solid-state NMR approaches that utilize stationary, aligned samples or...
Multi-receiver solid-state NMR using polarization optimized experiments (POE) at ultrafast magic angle spinning
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