Monitoring protein ubiquitination and SUMOylation in real-time by NMR.
Chem Commun (Camb). 2020 May 19;:
Authors: Habibullah BI, Tripathi V, Surana P, Das R
Abstract
The covalent conjugation of ubiquitin (Ub), known as ubiquitination, is a multi-step reaction involving multiple enzymes. We report a real-time, tag-free method to monitor protein ubiquitination by NMR spectroscopy under physiological conditions. The approach is also applicable for monitoring other ubiquitin-like modifications, and the disassembly of Ub polymers.
PMID: 32426760 [PubMed - as supplied by publisher]
Real-time monitoring of enzyme-assisted animal protein hydrolysis by NMR spectroscopy – An NMR reactomics concept
Real-time monitoring of enzyme-assisted animal protein hydrolysis by NMR spectroscopy – An NMR reactomics concept
Publication date: Available online 23 April 2018
Source:LWT</br>
Author(s): Ulrik K. Sundekilde, Lise Jarno, Nina Eggers, Hanne Christine Bertram</br>
This study presents an application of proton (1H) NMR spectroscopy for monitoring of enzyme-assisted hydrolysis of muscle protein under both at-line and real-time conditions. Measurements were carried out on sample material >1 g, and two different enzymes (alcalase and papain) were examined. The...
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04-23-2018 05:00 PM
Hyperpolarized 133Cs is a sensitive probe for real-time monitoring of biophysical environments
From The DNP-NMR Blog:
Hyperpolarized 133Cs is a sensitive probe for real-time monitoring of biophysical environments
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Karlsson, M., J.H. Ardenkjaer-Larsen, and M.H. Lerche, Hyperpolarized 133Cs is a sensitive probe for real-time monitoring of biophysical environments. Chemical Communications, 2017. 53(49): p. 6625-6628.
http://dx.doi.org/10.1039/C7CC02943H
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10-30-2017 10:19 PM
[NMR paper] In vivo NMR spectroscopy: toward real time monitoring of environmental stress.
In vivo NMR spectroscopy: toward real time monitoring of environmental stress.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-7315-19-Wiley_FullText_120x30_orange.png Related Articles In vivo NMR spectroscopy: toward real time monitoring of environmental stress.
Magn Reson Chem. 2015 Sep;53(9):774-9
Authors: Soong R, Nagato E, Sutrisno A, Fortier-McGill B, Akhter M, Schmidt S, Heumann H, Simpson AJ
PMID: 25296400
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05-18-2016 09:53 PM
[NMR paper] Real-time NMR monitoring of biological activities in complex physiological environments.
Real-time NMR monitoring of biological activities in complex physiological environments.
Real-time NMR monitoring of biological activities in complex physiological environments.
Curr Opin Struct Biol. 2015 Feb 26;32C:39-47
Authors: Smith MJ, Marshall CB, Theillet FX, Binolfi A, Selenko P, Ikura M
Abstract
Biological reactions occur in a highly organized spatiotemporal context and with kinetics that are modulated by multiple environmental factors. To integrate these variables in our experimental investigations of 'native'...
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03-03-2015 08:34 PM
[NMR paper] Real-time NMR monitoring of protein-folding kinetics by a recycle flow system for temperature jump.
Real-time NMR monitoring of protein-folding kinetics by a recycle flow system for temperature jump.
Real-time NMR monitoring of protein-folding kinetics by a recycle flow system for temperature jump.
Anal Chem. 2013 Sep 12;
Authors: Yamasaki K, Obara Y, Hasegawa M, Tanaka H, Yamasaki T, Wakuda T, Okada M, Kohzuma T
Abstract
An NMR method was developed that allows for real-time monitoring of reactions (on the order of seconds) induced by temperature jump. In a recycle flow system, heating and cooling baths were integrated, with the latter...
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09-14-2013 03:02 PM
Monitoring Mechanistic Details in the Synthesis of Pyrimidines via Real-Time, Ultrafast Multidimensional NMR Spectroscopy
Monitoring Mechanistic Details in the Synthesis of Pyrimidines via Real-Time, Ultrafast Multidimensional NMR Spectroscopy
Zulay D. Pardo, Gregory L. Olsen, Mari?a Encarnacio?n Ferna?ndez-Valle, Lucio Frydman, Roberto Marti?nez-A?lvarez and Antonio Herrera
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja210154g/aop/images/medium/ja-2011-10154g_0006.gif
Journal of the American Chemical Society
DOI: 10.1021/ja210154g
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/tEFGGh16-DU
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01-28-2012 05:27 AM
[NMR paper] Real time NMR monitoring of local unfolding of HIV-1 protease tethered dimer driven b
Real time NMR monitoring of local unfolding of HIV-1 protease tethered dimer driven by autolysis.
Related Articles Real time NMR monitoring of local unfolding of HIV-1 protease tethered dimer driven by autolysis.
FEBS Lett. 2001 May 18;497(1):59-64
Authors: Panchal SC, Bhavesh NS, Hosur RV
Structural studies in proteases have been hampered because of their inherent autolytic function. However, since autolysis is known to be mediated via protein unfolding, careful monitoring of the autolytic reaction has the potential to throw light on the...