The use of Nuclear Magnetic Resonance spectroscopy for studying lipid digestion in vitro most often consists of quantifying lipolysis products after they have been extracted from the reaction medium using organic solvents. However, the current sensitivity level of NMR spectrometers makes possible to avoid the extraction step and continuously quantify the lipids directly in the reaction medium. We used real-time ¹H NMR spectroscopy and guinea pig pancreatic lipase-related protein 2 (GPLRP2) as...
Real-time monitoring of enzyme-assisted animal protein hydrolysis by NMR spectroscopy – An NMR reactomics concept
Real-time monitoring of enzyme-assisted animal protein hydrolysis by NMR spectroscopy – An NMR reactomics concept
Publication date: Available online 23 April 2018
Source:LWT</br>
Author(s): Ulrik K. Sundekilde, Lise Jarno, Nina Eggers, Hanne Christine Bertram</br>
This study presents an application of proton (1H) NMR spectroscopy for monitoring of enzyme-assisted hydrolysis of muscle protein under both at-line and real-time conditions. Measurements were carried out on sample material >1 g, and two different enzymes (alcalase and papain) were examined. The...
nmrlearner
Journal club
0
04-23-2018 05:00 PM
[ASAP] Multiplexed Real-Time NMR GTPase Assay for Simultaneous Monitoring of Multiple Guanine Nucleotide Exchange Factor Activities from Human Cancer Cells and Organoids
Multiplexed Real-Time NMR GTPase Assay for Simultaneous Monitoring of Multiple Guanine Nucleotide Exchange Factor Activities from Human Cancer Cells and Organoids
Teklab Gebregiworgis, Christopher B. Marshall, Tadateru Nishikawa, Nikolina Radulovich, María-José Sandí, Zhenhao Fang, Robert Rottapel, Ming-Sound Tsao, Mitsuhiko Ikura
https://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.7b13703/20180322/images/medium/ja-2017-13703a_0005.gif
Journal of the American Chemical Society
DOI: 10.1021/jacs.7b13703...
nmrlearner
Journal club
0
03-22-2018 06:35 PM
[NMR paper] Multiplexed real-time NMR GTPase assay for simultaneous monitoring of multiple GEF activities from human cancer cells and organoids.
Multiplexed real-time NMR GTPase assay for simultaneous monitoring of multiple GEF activities from human cancer cells and organoids.
Multiplexed real-time NMR GTPase assay for simultaneous monitoring of multiple GEF activities from human cancer cells and organoids.
J Am Chem Soc. 2018 Mar 15;:
Authors: Gebregiworgis T, Marshall CB, Nishikawa T, Radulovich N, Sandi MJ, Fang Z, Rottapel R, Tsao MS, Ikura M
Abstract
Small GTPases (sGTPases) are critical switch-like regulators that mediate several important cellular functions and...
nmrlearner
Journal club
0
03-16-2018 12:12 PM
Salt-mediated oligomerization of the mouse prion protein monitored by real time NMR
Salt-mediated oligomerization of the mouse prion protein monitored by real time NMR
Publication date: Available online 11 May 2017
Source:Journal of Molecular Biology</br>
Author(s): Ishita Sengupta, Suhas H. Bhate, Ranabir Das, Jayant B. Udgaonkar</br>
The prion protein forms ß-rich soluble oligomers in vitro at pH4 in the presence of physiological concentrations of salt. In the absence of salt, oligomerization and misfolding does not take place in an experimentally tractable timescale. While it is well established that a lowering of pH facilitates...
nmrlearner
Journal club
0
05-11-2017 12:28 PM
[NMR paper] In vivo NMR spectroscopy: toward real time monitoring of environmental stress.
In vivo NMR spectroscopy: toward real time monitoring of environmental stress.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-7315-19-Wiley_FullText_120x30_orange.png Related Articles In vivo NMR spectroscopy: toward real time monitoring of environmental stress.
Magn Reson Chem. 2015 Sep;53(9):774-9
Authors: Soong R, Nagato E, Sutrisno A, Fortier-McGill B, Akhter M, Schmidt S, Heumann H, Simpson AJ
PMID: 25296400
nmrlearner
Journal club
0
05-18-2016 09:53 PM
[NMR paper] Real-Time Monitoring of New Delhi Metallo-?-Lactamase Activity in Living Bacterial Cells by (1) H NMR Spectroscopy.
Real-Time Monitoring of New Delhi Metallo-?-Lactamase Activity in Living Bacterial Cells by (1) H NMR Spectroscopy.
Related Articles Real-Time Monitoring of New Delhi Metallo-?-Lactamase Activity in Living Bacterial Cells by (1) H NMR Spectroscopy.
Angew Chem Int Ed Engl. 2014 Jan 23;
Authors: Ma J, McLeod S, Maccormack K, Sriram S, Gao N, Breeze AL, Hu J
Abstract
Disconnections between in vitro responses and those observed in whole cells confound many attempts to design drugs in areas of serious medical need. A method based on 1D (1) H...
nmrlearner
Journal club
0
01-25-2014 02:07 PM
Monitoring Mechanistic Details in the Synthesis of Pyrimidines via Real-Time, Ultrafast Multidimensional NMR Spectroscopy
Monitoring Mechanistic Details in the Synthesis of Pyrimidines via Real-Time, Ultrafast Multidimensional NMR Spectroscopy
Zulay D. Pardo, Gregory L. Olsen, Mari?a Encarnacio?n Ferna?ndez-Valle, Lucio Frydman, Roberto Marti?nez-A?lvarez and Antonio Herrera
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja210154g/aop/images/medium/ja-2011-10154g_0006.gif
Journal of the American Chemical Society
DOI: 10.1021/ja210154g
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/tEFGGh16-DU
Monitoring galactolipid digestion and simultaneous changes in lipid-bile salt micellar organization by real-time NMR spectroscopy
Linear Mode
