[NMR paper] Monitoring fast reactions by spatially-selective and frequency-shifted continuous NMR spectroscopy: application to rapid-injection protein unfolding.
Related ArticlesMonitoring fast reactions by spatially-selective and frequency-shifted continuous NMR spectroscopy: application to rapid-injection protein unfolding.
Chem Commun (Camb). 2013 Mar 12;
Authors: Wagner GE, Sakhaii P, Bermel W, Zangger K
Abstract
The repetition rate of an NMR experiment is usually limited by the longitudinal relaxation times of the investigated molecule. Here we show that continuous excitation and data acquisition, without any interscan delay, is possible by a spatially resolved experiment where different nuclei are excited in consecutive scans.
PMID: 23482928 [PubMed - as supplied by publisher]
Transforming between discrete and continuous angle distribution models: application to protein Ï?1 torsions
Transforming between discrete and continuous angle distribution models: application to protein Ï?1 torsions
Abstract Two commonly employed angular-mobility models for describing amino-acid side-chain Ï?1 torsion conformation, the staggered-rotamer jump and the normal probability density, are discussed and performance differences in applications to scalar-coupling data interpretation highlighted. Both models differ in their distinct statistical concepts, representing discrete and continuous angle distributions, respectively. Circular statistics, introduced for describing torsion-angle...
Rapid Injection NMR Reveals ?3 ‘?-Allyl’ CuIII Intermediates in Addition Reactions of Organocuprate Reagents
Rapid Injection NMR Reveals ?3 ‘?-Allyl’ CuIII Intermediates in Addition Reactions of Organocuprate Reagents
Steven H. Bertz, Richard A. Hardin, Michael D. Murphy, Craig A. Ogle, Joshua D. Richter and Andy A. Thomas
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja3022363/aop/images/medium/ja-2012-022363_0012.gif
Journal of the American Chemical Society
DOI: 10.1021/ja3022363
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/FjoEID-4z0g
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05-25-2012 07:14 PM
Frequency-selective heteronuclear dephasing and selective carbonyl labeling to deconvolute crowded spectra of membrane proteins by magic angle spinning NMR.
Frequency-selective heteronuclear dephasing and selective carbonyl labeling to deconvolute crowded spectra of membrane proteins by magic angle spinning NMR.
Frequency-selective heteronuclear dephasing and selective carbonyl labeling to deconvolute crowded spectra of membrane proteins by magic angle spinning NMR.
J Magn Reson. 2011 Mar 17;
Authors: Traaseth NJ, Veglia G
We present a new method that combines carbonyl-selective labeling with frequency-selective heteronuclear recoupling to resolve the spectral overlap of magic angle spinning (MAS) NMR...
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04-13-2011 11:57 PM
Frequency-Selective Heteronuclear Dephasing and Selective Carbonyl Labeling to Deconvolute Crowded Spectra of Membrane Proteins By Magic Angle Spinning NMR
Frequency-Selective Heteronuclear Dephasing and Selective Carbonyl Labeling to Deconvolute Crowded Spectra of Membrane Proteins By Magic Angle Spinning NMR
Publication year: 2011
Source: Journal of Magnetic Resonance, In Press, Accepted Manuscript, Available online 17 March 2011</br>
Nathaniel J., Traaseth , Gianluigi, Veglia</br>
We present a new method that combines carbonyl-selective labeling with frequency-selective heteronuclear recoupling to resolve the spectral overlap of magic angle spinning (MAS) NMR spectra of membrane proteins in fluid lipid membranes with broad lines and...
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03-18-2011 06:43 AM
Continuous-wave EPR at 275 GHz: Application to high-spin Fe3+ systems
Continuous-wave EPR at 275 GHz: Application to high-spin Fe3+ systems
Publication year: 2011
Source: Journal of Magnetic Resonance, In Press, Accepted Manuscript, Available online 11 March 2011</br>
G., Mathies , H., Blok , J.A.J.M., Disselhorst , P., Gast , H., van der Meer , ...</br>
The 275 GHz electron-paramagnetic-resonance spectrometer we reported on in 2004 has been equipped with a new probe head, which contains a cavity especially designed for operation in continuous-wave mode. The sensitivity and signal stability that is achieved with this new probe head is illustrated...
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03-12-2011 05:21 PM
[NMR paper] Combined frequency- and time-domain NMR spectroscopy. Application to fast protein res
Combined frequency- and time-domain NMR spectroscopy. Application to fast protein resonance assignment.
Related Articles Combined frequency- and time-domain NMR spectroscopy. Application to fast protein resonance assignment.
J Biomol NMR. 2004 May;29(1):57-64
Authors: Brutscher B
A simple and general method is presented to simplify multi-dimensional NMR spectra of isotope-labeled bio-molecules. The approach is based on band-selective Hadamard-type frequency encoding, which disperses the correlation peaks into different sub-spectra. This makes...
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11-24-2010 09:51 PM
[NMR paper] Protein expression, selective isotopic labeling, and analysis of hyperfine-shifted NM
Protein expression, selective isotopic labeling, and analysis of hyperfine-shifted NMR signals of Anabaena 7120 vegetative ferredoxin.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Protein expression, selective isotopic labeling, and analysis of hyperfine-shifted NMR signals of Anabaena 7120 vegetative ferredoxin.
Arch Biochem Biophys. 1995 Jan 10;316(1):619-34
Authors: Cheng H, Westler WM, Xia B, Oh BH, Markley JL
Two alternative T7 RNA promoter/polymerase systems...
Monitoring fast reactions by spatially-selective and frequency-shifted continuous NMR spectroscopy: application to rapid-injection protein unfolding.
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