Calcium carbonate (CaCO(3)) is one of the most significant biominerals in nature. Living organisms are able to control its biomineralization by means of an organic matrix to tailor a myriad of hybrid functional materials. The soluble organic components are often proteins rich in acidic amino-acids such as l-aspartic acid. While several studies have demonstrated the influence of amino acids on the crystallization of calcium carbonate, nanoscopic insight of their impact on CaCO(3) mineralization,...
[NMR paper] A mixing microfluidic chip for real-time NMR monitoring of macromolecular reaction
A mixing microfluidic chip for real-time NMR monitoring of macromolecular reaction
NMR spectroscopy permits real-time monitoring of reactions that involve changes in the spectra of reactants. MICCS (MIcro Channeled Cell for Synthesis monitoring) is a microfluidic chip for such purposes, which is used to rapidly activate reactions by mixing the reactant solutions in the chip inserted into the typical NMR tube. Although it allows monitoring of chemical reactions of small compounds, its simple mixing system dependent on diffusion in the microchannel was not suitable for...
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[NMR paper] Monitoring protein ubiquitination and SUMOylation in real-time by NMR.
Monitoring protein ubiquitination and SUMOylation in real-time by NMR.
Monitoring protein ubiquitination and SUMOylation in real-time by NMR.
Chem Commun (Camb). 2020 May 19;:
Authors: Habibullah BI, Tripathi V, Surana P, Das R
Abstract
The covalent conjugation of ubiquitin (Ub), known as ubiquitination, is a multi-step reaction involving multiple enzymes. We report a real-time, tag-free method to monitor protein ubiquitination by NMR spectroscopy under physiological conditions. The approach is also applicable for monitoring...
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05-20-2020 05:17 PM
Hyperpolarized 133Cs is a sensitive probe for real-time monitoring of biophysical environments
From The DNP-NMR Blog:
Hyperpolarized 133Cs is a sensitive probe for real-time monitoring of biophysical environments
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Karlsson, M., J.H. Ardenkjaer-Larsen, and M.H. Lerche, Hyperpolarized 133Cs is a sensitive probe for real-time monitoring of biophysical environments. Chemical Communications, 2017. 53(49): p. 6625-6628.
http://dx.doi.org/10.1039/C7CC02943H
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10-30-2017 10:19 PM
[NMR paper] In vivo NMR spectroscopy: toward real time monitoring of environmental stress.
In vivo NMR spectroscopy: toward real time monitoring of environmental stress.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-7315-19-Wiley_FullText_120x30_orange.png Related Articles In vivo NMR spectroscopy: toward real time monitoring of environmental stress.
Magn Reson Chem. 2015 Sep;53(9):774-9
Authors: Soong R, Nagato E, Sutrisno A, Fortier-McGill B, Akhter M, Schmidt S, Heumann H, Simpson AJ
PMID: 25296400
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05-18-2016 09:53 PM
[NMR paper] Real-time NMR monitoring of biological activities in complex physiological environments.
Real-time NMR monitoring of biological activities in complex physiological environments.
Real-time NMR monitoring of biological activities in complex physiological environments.
Curr Opin Struct Biol. 2015 Feb 26;32C:39-47
Authors: Smith MJ, Marshall CB, Theillet FX, Binolfi A, Selenko P, Ikura M
Abstract
Biological reactions occur in a highly organized spatiotemporal context and with kinetics that are modulated by multiple environmental factors. To integrate these variables in our experimental investigations of 'native'...
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03-03-2015 08:34 PM
[NMR paper] Characterization of the spherical intermediates and fibril formation of hCT in HEPES solution using solid-state 13C-NMR and transmission electron microscopy.
Characterization of the spherical intermediates and fibril formation of hCT in HEPES solution using solid-state 13C-NMR and transmission electron microscopy.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.rsc.org-images-entities-char_z_RSClogo.gif Related Articles Characterization of the spherical intermediates and fibril formation of hCT in HEPES solution using solid-state 13C-NMR and transmission electron microscopy.
Phys Chem Chem Phys. 2013 Oct 21;15(39):16956-64
Authors: Itoh-Watanabe H, Kamihira-Ishijima M,...
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04-22-2014 03:54 PM
[NMR paper] Real time NMR monitoring of local unfolding of HIV-1 protease tethered dimer driven b
Real time NMR monitoring of local unfolding of HIV-1 protease tethered dimer driven by autolysis.
Related Articles Real time NMR monitoring of local unfolding of HIV-1 protease tethered dimer driven by autolysis.
FEBS Lett. 2001 May 18;497(1):59-64
Authors: Panchal SC, Bhavesh NS, Hosur RV
Structural studies in proteases have been hampered because of their inherent autolytic function. However, since autolysis is known to be mediated via protein unfolding, careful monitoring of the autolytic reaction has the potential to throw light on the...
Monitoring of CaCO3 Nanoscale Structuration through Real-Time Liquid Phase Transmission Electron Microscopy and Hyperpolarized NMR
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