NMR spectroscopy permits real-time monitoring of reactions that involve changes in the spectra of reactants. MICCS (MIcro Channeled Cell for Synthesis monitoring) is a microfluidic chip for such purposes, which is used to rapidly activate reactions by mixing the reactant solutions in the chip inserted into the typical NMR tube. Although it allows monitoring of chemical reactions of small compounds, its simple mixing system dependent on diffusion in the microchannel was not suitable for...
[NMR paper] Monitoring protein ubiquitination and SUMOylation in real-time by NMR.
Monitoring protein ubiquitination and SUMOylation in real-time by NMR.
Monitoring protein ubiquitination and SUMOylation in real-time by NMR.
Chem Commun (Camb). 2020 May 19;:
Authors: Habibullah BI, Tripathi V, Surana P, Das R
Abstract
The covalent conjugation of ubiquitin (Ub), known as ubiquitination, is a multi-step reaction involving multiple enzymes. We report a real-time, tag-free method to monitor protein ubiquitination by NMR spectroscopy under physiological conditions. The approach is also applicable for monitoring...
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05-20-2020 05:17 PM
Shim-on-Chip Design for Microfluidic NMR Detectors
From The DNP-NMR Blog:
Shim-on-Chip Design for Microfluidic NMR Detectors
Active shims to achieve high-resolution spectra are crucial parts of the NMR instrumentation. Typically, shims are designed to produce a magnetic correction field corresponding to individual spherical harmonics. Other methods have been proposed (e.g. matrix shims) and the approach described in this article simplify uses flat ribbon cables.
Meerten, S. G. J. van, P. J. M. van Bentum, and A. P. M. Kentgens. “Shim-on-Chip Design for Microfluidic NMR Detectors.” Analytical Chemistry 90, no. 17 (September 4,...
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04-03-2020 09:41 PM
[NMR paper] Optimized fast mixing device for real-time NMR applications.
Optimized fast mixing device for real-time NMR applications.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Optimized fast mixing device for real-time NMR applications.
J Magn Reson. 2017 May 31;281:125-129
Authors: Franco R, Favier A, Schanda P, Brutscher B
Abstract
We present an improved fast mixing device based on the rapid mixing of two solutions inside the NMR probe, as originally proposed by Hore and coworkers (J. Am. Chem. Soc. 125...
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06-10-2017 05:21 PM
[NMR paper] Optimized fast mixing device for real-time NMR applications
Optimized fast mixing device for real-time NMR applications
Publication date: Available online 31 May 2017
Source:Journal of Magnetic Resonance</br>
Author(s): Rémi Franco, Adrien Favier, Paul Schanda, Bernhard Brutscher</br>
We present an improved fast mixing device based on the rapid mixing of two solutions inside the NMR probe, as originally proposed by Hore and coworkers (J. Am. Chem. Soc. 125 (2003) 12484–12492). Such a device is important for off-equilibrium studies of molecular kinetics by multidimensional real-time NMR spectrsocopy. The novelty of...
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05-31-2017 08:35 AM
[NMR paper] In vivo NMR spectroscopy: toward real time monitoring of environmental stress.
In vivo NMR spectroscopy: toward real time monitoring of environmental stress.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-7315-19-Wiley_FullText_120x30_orange.png Related Articles In vivo NMR spectroscopy: toward real time monitoring of environmental stress.
Magn Reson Chem. 2015 Sep;53(9):774-9
Authors: Soong R, Nagato E, Sutrisno A, Fortier-McGill B, Akhter M, Schmidt S, Heumann H, Simpson AJ
PMID: 25296400
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05-18-2016 09:53 PM
PhD scholarship in Development of DNP-NMR Methods for the Real-Time Investigation of Catalytic Reaction Mechanisms
From The DNP-NMR Blog:
PhD scholarship in Development of DNP-NMR Methods for the Real-Time Investigation of Catalytic Reaction Mechanisms
A 3-year PhD scholarship is available from the 1 September 2015 or as soon as possible thereafter at Department of Chemistry at Technical University of Denmark and DTU Electro.
The project is funded as a part of the Danish National Research Foundation Center of Excellence Center for Hyperpolarized Magnetic Resonance:http://www.dnp.dtu.dk/
A mixing microfluidic chip for real-time NMR monitoring of macromolecular reaction
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