NMR paper Milligram-Scale Assembly and NMR Fingerprint of Tau Fibrils Adopting the Alzheimer's Disease Fold

		BioNMR > Educational resources > Journal club
[NMR paper] Milligram-Scale Assembly and NMR Fingerprint of Tau Fibrils Adopting the Alzheimer's Disease Fold

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

04-29-2024, 09:50 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,734

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Milligram-Scale Assembly and NMR Fingerprint of Tau Fibrils Adopting the Alzheimer's Disease Fold

Milligram-Scale Assembly and NMR Fingerprint of Tau Fibrils Adopting the Alzheimer's Disease Fold

In the Alzheimer's disease (AD) brain, the microtubule-associated protein tau aggregates into paired helical filaments (PHFs) in which each protofilament has a C-shaped conformation. In vitro assembly of tau fibrils adopting this fold is highly valuable for both fundamental and applied studies of AD without requiring patient-brain extracted fibrils. To date, reported methods for forming AD-fold tau fibrils have been irreproducible and sensitive to subtle variations in fibrillization conditions....

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Cell-free synthesis of amyloid fibrils with infectious properties and amenable to sub-milligram magic-angle spinning NMR analysis Cell-free synthesis of amyloid fibrils with infectious properties and amenable to sub-milligram magic-angle spinning NMR analysis Structural investigations of amyloid fibrils often rely on heterologous bacterial overexpression of the protein of interest. Due to their inherent hydrophobicity and tendency to aggregate as inclusion bodies, many amyloid proteins are challenging to express in bacterial systems. Cell-free protein expression is a promising alternative to classical bacterial expression to produce hydrophobic proteins and introduce NMR-active isotopes that can improve and speed up...	nmrlearner	Journal club	0	11-10-2022 06:00 AM
Magic-angle-spinning NMR used to probe protein/microtubule assembly at atomic scale - UD Daily Magic-angle-spinning NMR used to probe protein/microtubule assembly at atomic scale - UD Daily Magic-angle-spinning NMR used to probe protein/microtubule assembly at atomic scale UD Daily5:30 p.m., Nov. 24, 2015--A latticework of tiny tubes called microtubules gives your cells their shape and also acts like a railroad track that essential proteins ... Read here	nmrlearner	Online News	0	01-07-2019 05:49 AM
Magic-angle-spinning NMR used to probe protein/microtubule assembly at atomic scale - Phys.Org Magic-angle-spinning NMR used to probe protein/microtubule assembly at atomic scale - Phys.Org http://www.bionmr.com//t1.gstatic.com/images?q=tbn:ANd9GcRBtQa7jjiFR1Ka7bIeJtv8GwyoUep-8VjT60fBCPfstugvxkbxBGj14wdtmcsu0NjpeAQ5zKru Phys.Org <img alt="" height="1" width="1"> Magic-angle-spinning NMR used to probe protein/microtubule assembly at atomic scale Phys.Org "Just as we are always moving our arms and legs about, proteins are very dynamic. They do not stand still," Polenova says. "These motions are essential to their biological function, and NMR spectroscopy is the only technique...	nmrlearner	Online News	0	12-02-2017 02:54 PM
Cryo-electron microscopy: Alzheimer's fibrils Cryo-electron microscopy: Alzheimer's fibrils http://www.spectroscopynow.com/common/images/thumbnails/15ec800ad6f.jpgA technique that can augment X-ray crystallographic and spectroscopic studies has been demonstrated to provide an atomic resolution in imaging of the amyloid fibrils present in Alzheimer's disease, thanks to European research. Read the rest at Spectroscopynow.com	nmrlearner	General	0	10-01-2017 06:06 AM
[NMR paper] Capturing a reactive state of amyloid aggregates: NMR-based characterization of copper-bound Alzheimer disease amyloid ?-fibrils in a redox cycle. Capturing a reactive state of amyloid aggregates: NMR-based characterization of copper-bound Alzheimer disease amyloid ?-fibrils in a redox cycle. http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--highwire.stanford.edu-icons-externalservices-pubmed-standard-jbc_final.gif Related Articles Capturing a reactive state of amyloid aggregates: NMR-based characterization of copper-bound Alzheimer disease amyloid ?-fibrils in a redox cycle. J Biol Chem. 2014 Apr 4;289(14):9998-10010 Authors: Parthasarathy S, Yoo B, McElheny D, Tay W,...	nmrlearner	Journal club	0	05-31-2014 01:57 PM
[NMR paper] Cryogenic solid state NMR studies of fibrils of the Alzheimer's disease amyloid-? peptide: perspectives for DNP. Cryogenic solid state NMR studies of fibrils of the Alzheimer's disease amyloid-? peptide: perspectives for DNP. http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles Cryogenic solid state NMR studies of fibrils of the Alzheimer's disease amyloid-? peptide: perspectives for DNP. J Biomol NMR. 2013 Aug;56(4):359-63 Authors: Lopez del Amo JM, Schneider D, Loquet A, Lange A, Reif B Abstract Dynamic Nuclear Polarization solid-state NMR...	nmrlearner	Journal club	0	05-23-2014 03:21 PM
Cryogenic solid state NMR studies of fibrils of the Alzheimer's disease amyloid-beta peptide: perspectives for DNP From The DNP-NMR Blog: Cryogenic solid state NMR studies of fibrils of the Alzheimer's disease amyloid-beta peptide: perspectives for DNP Lopez Del Amo, J.M., et al., Cryogenic solid state NMR studies of fibrils of the Alzheimer's disease amyloid-beta peptide: perspectives for DNP. J Biomol NMR, 2013: p. 1-5. http://www.ncbi.nlm.nih.gov/pubmed/23793606	nmrlearner	News from NMR blogs	0	08-14-2013 05:24 PM
Cryogenic solid state NMR studies of fibrils of the Alzheimer's disease amyloid-beta peptide: perspectives for DNP From The DNP-NMR Blog: Cryogenic solid state NMR studies of fibrils of the Alzheimer's disease amyloid-beta peptide: perspectives for DNP Lopez del Amo, J.-M., et al., Cryogenic solid state NMR studies of fibrils of the Alzheimer’s disease amyloid-? peptide: perspectives for DNP. J. Biomol. NMR, 2013: p. 1-5. http://www.ncbi.nlm.nih.gov/pubmed/23793606	nmrlearner	News from NMR blogs	0	07-19-2013 09:20 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off