Fluorine (19F) NMR is emerging as an invaluable analytical technique in chemistry, biochemistry, structural biology, material science, drug discovery, and medicine, especially due to the inherent rarity of naturally occurring fluorine in biological, organic, and inorganic compounds. Here, we revisit the under-reported problem of fluoride leaching from new and unused glass NMR tubes. We characterised the leaching of free fluoride from various types of new and unused glass NMR tubes over the course of several hours and quantify this contaminant to be at micromolar concentrations for typical NMR sample volumes across multiple glass types and brands. We find that this artefact is undetectable for samples prepared in quartz NMR tubes within the timeframes of our experiments. We also observed that pre-soaking new glass NMR tubes combined with rinsing removes this contamination below micromolar levels. Given the increasing popularity of 19F NMR across a wide range of fields, increasing popularity of single-use screening tubes, the long collection times required for relaxation studies and samples of low concentrations, and the importance of avoiding contamination in all NMR experiments, we anticipate that our simple solution will be useful to biomolecular NMR spectroscopists.
[NMR paper] Fragment Screening and Fast Micromolar Detection on a Benchtop NMR Spectrometer Boosted by Photoinduced Hyperpolarization
Fragment Screening and Fast Micromolar Detection on a Benchtop NMR Spectrometer Boosted by Photoinduced Hyperpolarization
Fragment-based drug design is a well-established strategy for rational drug design, with nuclear magnetic resonance (NMR) on high-field spectrometers as the method of reference for screening and hit validation. However, high-field NMR spectrometers are not only expensive, but require specialized maintenance, dedicated space, and depend on liquid helium cooling which became critical over the recurring global helium shortages. We propose an alternative to high-field NMR...
[NMR paper] Applications of high dimensionality experiments to biomolecular NMR.
Applications of high dimensionality experiments to biomolecular NMR.
Applications of high dimensionality experiments to biomolecular NMR.
Prog Nucl Magn Reson Spectrosc. 2015 Nov;90-91:49-73
Authors: Nowakowski M, Saxena S, Stanek J, ?erko S, Ko?mi?ski W
Abstract
High dimensionality NMR experiments facilitate resonance assignment and precise determination of spectral parameters such as coupling constants. Sparse non-uniform sampling enables acquisition of experiments of high dimensionality with high resolution in acceptable...
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11-26-2015 12:13 AM
Applications of high dimensionality experiments to biomolecular NMR
Applications of high dimensionality experiments to biomolecular NMR
Publication date: Available online 11 July 2015
Source:Progress in Nuclear Magnetic Resonance Spectroscopy</br>
Author(s): Micha? Nowakowski , Saurabh Saxena , Jan Stanek , Szymon ?erko , Wiktor Ko?mi?ski</br>
High dimensionality NMR experiments facilitate resonance assignment and precise determination of spectral parameters such as coupling constants. Sparse non-uniform sampling enables acquisition of experiments of high dimensionality with high resolution in acceptable time. In this review...
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07-12-2015 07:12 AM
A Simple Device for Cleaning Many NMR Tubes Simultaneously
A Simple Device for Cleaning Many NMR Tubes Simultaneously
http://www.spectroscopynow.com/common/images/thumbnails/sepspec19674education.jpgNMR tubes are widely used in organic chemistry. Cleaning a few tubes after use is not a big problem but cleaning hundreds of tubes is a demanding task in terms of time and use of solvents. Here is presented a simple nmr tube cleaner that can clean and dry hundreds of tubes in a short time with minimal exposure of the user to solvents. The tube cleaner is easily assembled from equipment normally present in the organic chemistry laboratory.
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02-03-2013 09:28 AM
[Question from NMRWiki Q&A forum] Diamagnetic contamination
Diamagnetic contamination
How to determine diamagnetic contamination in PRE experiments.In what extent it will effect the Gamma2 values?
Check if somebody has answered this question on NMRWiki QA forum
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12-14-2012 08:57 PM
[NMR paper] An emerging concept of biomolecular dynamics and function: applications of NMR & MRI.
An emerging concept of biomolecular dynamics and function: applications of NMR & MRI.
Related Articles An emerging concept of biomolecular dynamics and function: applications of NMR & MRI.
Magn Reson Med Sci. 2002;1(1):27-31
Authors: Kuwata K
A new concept of protein dynamics has emerged quite recently, and a crucial link between protein dynamics and function has been largely established using recent NMR techniques in the solution state. Protein structure is governed by the thermodynamic principle and may not necessarily be unique in the...
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11-24-2010 08:49 PM
[NMR paper] Refinement of the NMR solution structure of a protein to remove distortions arising f
Refinement of the NMR solution structure of a protein to remove distortions arising from neglect of internal motion.
Related Articles Refinement of the NMR solution structure of a protein to remove distortions arising from neglect of internal motion.
Biochemistry. 1991 Apr 23;30(16):3807-11
Authors: Fejzo J, Krezel AM, Westler WM, Macura S, Markley JL
The effect of internal motion on the quality of a protein structure derived from nuclear magnetic resonance (NMR) cross relaxation has been investigated experimentally. Internal rotation of the...
Micromolar fluoride contamination arising from glass NMR tubes and a simple solution for biomolecular applications
Linear Mode
