Related ArticlesMicelle-induced curvature in a water-insoluble HIV-1 Env peptide revealed by NMR dipolar coupling measurement in stretched polyacrylamide gel.
The structure of a water-insoluble fragment encompassing residues 282-304 of the HIV envelope protein gp41 is studied when solubilized by dihexanoyl phosphatidylcholine (DHPC) and by small bicelles, consisting of a 4:1 molar ratio of DHPC and dimyristoyl phosphatidylcholine (DMPC). Weak alignment with the magnetic field was accomplished in a stretched polyacrylamide gel, permitting measurement of one-bond (1)H-(15)N, (13)Ca-(13)C', and (13)C'-(15)N dipolar couplings, which formed the basis for determining the peptide structure. In both detergent systems, the peptide adopts an alpha-helical conformation from residue 4 through 18. In the presence of the DHPC micelles the helix is strongly curved towards the hydrophobic surface, whereas in the presence of bicelles a much weaker curvature in the opposite direction is observed.
Helix conformation of a small peptide melittin in a methanol-water mixed solvent studied by NMR.
Helix conformation of a small peptide melittin in a methanol-water mixed solvent studied by NMR.
Helix conformation of a small peptide melittin in a methanol-water mixed solvent studied by NMR.
Protein Pept Lett. 2011 Mar;18(3):318-26
Authors: Miura Y
Temperature dependence of the ?-helix conformation of bee venom melittin in methanol-water mixed solvents has been examined by NMR, in order to elucidate conformation stability and a phase diagram. At high methanol concentration of 100 - ca. 80 wt.%, melittin forms a full ?-helix conformation in the...
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06-04-2011 11:26 AM
[NMR paper] NMR structural analysis of a peptide mimic of the bridging sheet of HIV-1 gp120 in methanol and water.
NMR structural analysis of a peptide mimic of the bridging sheet of HIV-1 gp120 in methanol and water.
Related Articles NMR structural analysis of a peptide mimic of the bridging sheet of HIV-1 gp120 in methanol and water.
Biochem J. 2005 Sep 1;390(Pt 2):573-81
Authors: Chakraborty K, Shivakumar P, Raghothama S, Varadarajan R
gp120 is a subunit of the Env (viral envelope protein) of HIV-1. The protein consists of inner and outer domains linked by a bridging sheet. Several gp120 residues that bind the neutralizing antibody 17b as well as the...
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[NMR paper] 'Boomerang'-like insertion of a fusogenic peptide in a lipid membrane revealed by sol
'Boomerang'-like insertion of a fusogenic peptide in a lipid membrane revealed by solid-state 19F NMR.
Related Articles 'Boomerang'-like insertion of a fusogenic peptide in a lipid membrane revealed by solid-state 19F NMR.
Magn Reson Chem. 2004 Feb;42(2):195-203
Authors: Afonin S, Dürr UH, Glaser RW, Ulrich AS
Solid state (19)F NMR revealed the conformation and alignment of the fusogenic peptide sequence B18 from the sea urchin fertilization protein bindin embedded in flat phospholipid bilayers. Single (19)F labels were introduced into nine...
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11-24-2010 09:25 PM
[NMR paper] Micelle-bound conformation of a hairpin-forming peptide: combined NMR and molecular d
Micelle-bound conformation of a hairpin-forming peptide: combined NMR and molecular dynamics study.
Related Articles Micelle-bound conformation of a hairpin-forming peptide: combined NMR and molecular dynamics study.
Biopolymers. 2002 Nov 15;65(4):284-98
Authors: Dixon AM, Venable RM, Pastor RW, Bull TE
A peptide fragment from a protein hairpin turn region was modified by addition of isoleucine residues to both ends to enhance binding to lipid micelles; the resulting peptide (I(1)-I(2)-C(3)-N(4)-N(5)-P(6)-H(7)-I(8)-I(9)) contains the core...
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Production of recombinant isotopically labelled peptide by fusion to an insoluble par
Production of recombinant isotopically labelled peptide by fusion to an insoluble partner protein: generation of integrin ?v?6 binding peptides for NMR.
Related Articles Production of recombinant isotopically labelled peptide by fusion to an insoluble partner protein: generation of integrin ?v?6 binding peptides for NMR.
Mol Biosyst. 2010 Oct 18;
Authors: Wagstaff JL, Howard MJ, Williamson RA
The integrin ?v?6 is up-regulated in several cancers and has clinical potential for both tumour imaging and therapy. Peptide ligands have been developed...
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10-19-2010 04:51 PM
[NMR paper] 2D NMR and structural model for a mitochondrial signal peptide bound to a micelle.
2D NMR and structural model for a mitochondrial signal peptide bound to a micelle.
Related Articles 2D NMR and structural model for a mitochondrial signal peptide bound to a micelle.
Biochemistry. 1990 Oct 23;29(42):9872-8
Authors: Karslake C, Piotto ME, Pak YK, Weiner H, Gorenstein DG
The 19 amino acid signal peptide of rat liver aldehyde dehydrogenase, possessing a lysine substitution for an arginine and containing 3 extra amino acid residues at the C terminus, was studied by two-dimensional NMR in a dodecylphosphocholine micelle. In this...
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08-21-2010 11:04 PM
[NMR paper] Calcium-induced peptide association to form an intact protein domain: 1H NMR structur
Calcium-induced peptide association to form an intact protein domain: 1H NMR structural evidence.
Related Articles Calcium-induced peptide association to form an intact protein domain: 1H NMR structural evidence.
Science. 1990 Jul 20;249(4966):280-3
Authors: Shaw GS, Hodges RS, Sykes BD
The 70-residue carboxyl-terminal domain of the muscle contractile protein troponin-C contains two helix-loop-helix calcium (Ca)-binding sites that are related to each other by approximate twofold rotational symmetry. Hydrophobic residues from the helices and a...
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[NMR paper] An intrinsic curvature towards the minor groove in the cAMP-responsive element DNA fo
An intrinsic curvature towards the minor groove in the cAMP-responsive element DNA found by combined NMR and molecular modelling studies.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles An intrinsic curvature towards the minor groove in the cAMP-responsive element DNA found by combined NMR and molecular modelling studies.
Eur J Biochem. 1999 Feb;259(3):877-86
Authors: Chaoui M, Derreumaux S, Mauffret O, Fermandjian S
The...
Micelle-induced curvature in a water-insoluble HIV-1 Env peptide revealed by NMR dipo
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