Abstract
This work is the first comprehensive report on the quantitative metabolomic composition of the rat lens. Quantitative metabolomic profiles of lenses were acquired with the combined use of high-frequency nuclear magnetic resonance (NMR) and high-performance liquid chromatography with high-resolution mass-spectrometric detection (LC-MS) methods. More than forty low molecular weight compounds found in the lens have been reliably identified and quantified. The most abundant metabolites in the 3-month-old Wistar rat lens are taurine, hypotaurine, lactate, phosphocholine and reduced glutathione. The analysis of age-related changes in the lens metabolomic composition shows a gradual decrease of the content of most metabolites. This decrease is the most pronounced between 1 and 3 months, which probably corresponds to the completion of the lens maturation in one-month-old rats and to the high rate of the young lens growth. The enhanced levels of tryptophan, tyrosine, carnitine, glycerophosphate, GSH and GSSG were found in lenses of senescence-accelerated OXYS rats; for some metabolites, this effect may probably be attributed to the compensatory response to oxidative stress.
[NMR paper] An NMR-based metabolomics study of pork from different crossbreeds and relation to sensory perception.
An NMR-based metabolomics study of pork from different crossbreeds and relation to sensory perception.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles An NMR-based metabolomics study of pork from different crossbreeds and relation to sensory perception.
Meat Sci. 2014 Feb;96(2 Pt A):719-28
Authors: Straadt IK, Aaslyng MD, Bertram HC
Abstract
Meat extracts from five different pig crossbreeds including Duroc/Landrace/Yorkshire (DLY),...
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07-30-2014 10:22 AM
TransientElectrostatic Interactions Dominate theConformational Equilibrium Sampled by Multidomain Splicing FactorU2AF65: A Combined NMR and SAXS Study
TransientElectrostatic Interactions Dominate theConformational Equilibrium Sampled by Multidomain Splicing FactorU2AF65: A Combined NMR and SAXS Study
Jie-rong Huang, Lisa R. Warner, Carolina Sanchez, Frank Gabel, Tobias Madl, Cameron D. Mackereth, Michael Sattler and Martin Blackledge
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja502030n/aop/images/medium/ja-2014-02030n_0009.gif
Journal of the American Chemical Society
DOI: 10.1021/ja502030n
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04-30-2014 02:03 AM
[NMR paper] pH-Independence of trialanine and the effects of termini blocking in short peptides: a combined vibrational, NMR, UVCD, and molecular dynamics study.
pH-Independence of trialanine and the effects of termini blocking in short peptides: a combined vibrational, NMR, UVCD, and molecular dynamics study.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles pH-Independence of trialanine and the effects of termini blocking in short peptides: a combined vibrational, NMR, UVCD, and molecular dynamics study.
J Phys Chem B. 2013 Apr 11;117(14):3689-706
Authors: Toal S, Meral D, Verbaro D, Urbanc B, Schweitzer-Stenner R
Abstract...
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09-24-2013 10:18 AM
[NMR paper] Impact of oxidized phospholipids on the structural and dynamic organization of phospholipid membranes: a combined DSC and solid state NMR study.
Impact of oxidized phospholipids on the structural and dynamic organization of phospholipid membranes: a combined DSC and solid state NMR study.
Impact of oxidized phospholipids on the structural and dynamic organization of phospholipid membranes: a combined DSC and solid state NMR study.
Faraday Discuss. 2013;161:499-513; discussion 563-89
Authors: Wallgren M, Beranova L, Pham QD, Linh K, Lidman M, Procek J, Cyprych K, Kinnunen PK, Hof M, Gröbner G
Abstract
Membranes undergo severe changes under oxidative stress conditions due to the...
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06-29-2013 11:49 AM
[NMR paper] Direct Evidence for Hydrogen Bonding Between Hydroxyl Groups in Glycans: A Combined NMR and Molecular Dynamics Study.
Direct Evidence for Hydrogen Bonding Between Hydroxyl Groups in Glycans: A Combined NMR and Molecular Dynamics Study.
Related Articles Direct Evidence for Hydrogen Bonding Between Hydroxyl Groups in Glycans: A Combined NMR and Molecular Dynamics Study.
J Phys Chem B. 2013 Mar 26;
Authors: Battistel MD, Pendrill R, Widmalm G, Freedberg DI
Abstract
With this report we introduce the abundant hydroxyl groups of glycans as NMR handles and structural probes that expand the repertoire of tools for structure-function studies on glycans in...
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03-28-2013 04:03 PM
1H NMR Metabolomics Study of Human Urine
1H NMR Metabolomics Study of Human Urine
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1H NMR Metabolomics Study of Human Urine
A presentation of some recent work from the Metabolomics and Bioinformatics & Statistics Groups at the Institute of Food Research, Norwich, UK. See the teams' webpages at: www.ifr.ac.uk and www.ifr.ac.uk This video is a shortened version of a presentation given at "Advances in Metabolic Profiling 2011".
From:BioStatsXtra
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07-30-2012 07:42 AM
Methanol Strengthens Hydrogen Bonds and Weakens Hydrophobic Interactions in Proteins - A Combined Molecular Dynamics and NMR study.
Methanol Strengthens Hydrogen Bonds and Weakens Hydrophobic Interactions in Proteins - A Combined Molecular Dynamics and NMR study.
Methanol Strengthens Hydrogen Bonds and Weakens Hydrophobic Interactions in Proteins - A Combined Molecular Dynamics and NMR study.
J Phys Chem B. 2011 May 2;
Authors: Hwang S, Shao Q, Williams H, Hilty C, Gao YQ
A combined simulation and experimental study was performed to investigate how methanol affects the structure of a model peptide BBA5. BBA5 forms a stable ?-hairpin-?-helix structure in aqueous solutions....
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05-04-2011 04:14 PM
Sequence-Dependent Enrichment of a Model Phosphopeptide: A Combined MALDI-TOF and NMR Study.
Sequence-Dependent Enrichment of a Model Phosphopeptide: A Combined MALDI-TOF and NMR Study.
Sequence-Dependent Enrichment of a Model Phosphopeptide: A Combined MALDI-TOF and NMR Study.
Anal Chem. 2011 Mar 23;
Authors: Lucre?ce M, Emmanuelle S, Fabienne B, Sandrine S, Olivier L, Ge?rard B
The goal of this study was to detect and quantify by MALDI-TOF MS the phosphorylation of a peptide containing the recognition motif of the Protein Kinase C (PKC). Such model peptide can be used as a phosphorylation probe to follow intracellular...
Metabolomics of the rat lens: a combined LC-MS and NMR study.
Linear Mode
