[NMR paper] Metabolomic investigation of Arthus reaction in a rat model using proton nuclear magnetic resonance (1H NMR) spectroscopy and rapid resolution liquid chromatography (RRLC).
Metabolomic investigation of Arthus reaction in a rat model using proton nuclear magnetic resonance (1H NMR) spectroscopy and rapid resolution liquid chromatography (RRLC).
Related ArticlesMetabolomic investigation of Arthus reaction in a rat model using proton nuclear magnetic resonance (1H NMR) spectroscopy and rapid resolution liquid chromatography (RRLC).
Mol Biosyst. 2013 Jun;9(6):1423-35
Authors: Zhang X, Shen J, Cao B, Xu L, Zhao T, Liu X, Zhang H
Abstract
Arthus reaction (AR), a type of unconventional immune complex-mediated inflammation, is likely accompanied by alterations in circulating metabolites. Here, a proton nuclear magnetic resonance ((1)H NMR) spectroscopy method coupled with a rapid resolution liquid chromatography (RRLC) method was developed to evaluate the systemic metabolic consequences of AR and characterize metabolic aberrations. Serum and urine samples from AR rats and normal controls were compared to determine whether there were significant alterations associated with AR. The partial least squares discriminant analysis (PLS-DA) models of metabolomic results demonstrated good intergroup separations between AR rats and normal controls. Multivariate statistical analysis revealed significant alterations in the levels of 34 metabolites, which were termed as the disease-associated biomarkers. Differential metabolites identified from the metabolomic analysis suggested that AR caused dysfunctions of kidney and liver accompanied with changes in widespread metabolic pathways including the tricarboxylic acid (TCA) cycle, gut microbiota metabolism, lipids and cell membranes metabolism, glucose metabolism, fatty acid ?-oxidation, amino acids metabolism and ketogenesis. This study assessed and provided important metabolomic variations in serum and urine associated with AR and, therefore, demonstrated metabolomics as a powerful approach for the complete elucidation of the underlying pathophysiologic mechanisms of AR.
Towards automatic metabolomic profiling of high-resolution one-dimensional proton NMR spectra
Towards automatic metabolomic profiling of high-resolution one-dimensional proton NMR spectra
Abstract Nuclear magnetic resonance (NMR) and Mass Spectroscopy (MS) are the two most common spectroscopic analytical techniques employed in metabolomics. The large spectral datasets generated by NMR and MS are often analyzed using data reduction techniques like Principal Component Analysis (PCA). Although rapid, these methods are susceptible to solvent and matrix effects, high rates of false positives, lack of reproducibility and limited data transferability from one platform to the next....
Metabolomic investigation of Arthus reaction in a rat model using proton nuclear magnetic resonance (1H NMR) spectroscopy and rapid resolution liquid chromatography (RRLC).
Linear Mode
