Related ArticlesMetabolic profiling in kidneys of Atlantic salmon infected with Aeromonas salmonicida based on (1)H NMR.
Fish Shellfish Immunol. 2016 Aug 27;
Authors: Liu PF, Du Y, Meng L, Li X, Liu Y
Abstract
Aeromonas salmonicida, an important pathogenic bacterium which induces furunculosis, is globally causing increased risks in Atlantic salmon (Salmo salar) farming. Although the kidney is the main target organ of A. salmonicida, the metabolic profiling of kidney in response to A. salmonicida in vivo remains unknown. Here, we used (1)H nuclear magnetic resonance (NMR) to comprehensively analyze the metabolic changes in the kidney of Atlantic salmon. Through the NOESYPR1D spectrum combined with multi-variate pattern recognition analysis, including principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) models, significant metabolic changes were observed seven and 14 days post-infection and in a control group. Hence, the main objective of this study was to estimate the significant metabolites with resistance to furunculosis and further understand the mechanism of A. salmonicida in Atlantic salmon. Notably, substantial alterations of kidney metabolites were observed, such as with fumarate, alanine, valine, glycine, aspartate, choline, glycerophosphocholine and betaine, and summarized by metabolic pathways including the citrate cycle, glycolysis/gluconeogenesis, tryptophan metabolism, and urea cycle, respectively. Changes were also observed in 3-hydroxybutyrate and phosphocholine which were not involved in these four metabolic pathways. After analyzing the alteration trend of these metabolites, we inferred that A. salmonicida caused absorption inhibition of amino acids and disturbed protein metabolism as well as cell metabolism in favor of its replication. These observations offered novel insights into the mechanisms of infection at a functional level and facilitated further assessment and clarification of fish disease from A. salmonicida exposure.
PMID: 27577538 [PubMed - as supplied by publisher]
[NMR paper] NMR-Based Metabolic Profiling Reveals Neurochemical Alterations in the Brain of Rats Treated with Sorafenib.
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http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles NMR-Based Metabolic Profiling Reveals Neurochemical Alterations in the Brain of Rats Treated with Sorafenib.
Neurotox Res. 2015 Nov;28(4):290-301
Authors: Du C, Shao X, Zhu R, Li Y, Zhao Q, Fu D, Gu H, Kong J, Luo L, Long H, Deng P, Wang H, Hu C, Zhao Y, Cen X
Abstract
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07-22-2016 02:21 PM
[NMR paper] A pilot metabolic profiling study in hepatopancreas of Litopenaeus vannamei with white spot syndrome virus based on (1)H NMR spectroscopy.
A pilot metabolic profiling study in hepatopancreas of Litopenaeus vannamei with white spot syndrome virus based on (1)H NMR spectroscopy.
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J Invertebr Pathol. 2014 Oct 14;124C:51-56
Authors: Liu PF, Liu QH, Wu Y, Jie H
Abstract
White spot syndrome virus, which was a pathogen first found in 1992, had emerged globally affecting shrimp populations in aquaculture. Here, we comprehensively...
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12-03-2014 04:05 PM
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Mol Cancer. 2014 Aug 27;13(1):197
Authors: Shao W, Gu J, Huang C, Liu D, Huang H, Huang Z, Lin Z, Yang W, Liu K, Lin D, Ji T
Abstract
BACKGROUND: Ambiguity in malignant transformation of glioma has made prognostic diagnosis very challenging. Tumor malignant transformation is closely correlated with specific alterations...
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08-29-2014 05:36 PM
Journal Highlight: 1H NMR based metabolic profiling in Crohn's disease by random forest methodology
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http://www.spectroscopynow.com/common/images/thumbnails/146ed5b84e7.jpg1H NMR spectroscopy of the serum metabolites of patients with Crohn's disease, supported by random forest methodology, showed that valine and isoleucine are differentiating metabolites for diagnosis.
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06-30-2014 11:39 PM
Colon cancer calculated: Metabolic profiling
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http://www.spectroscopynow.com/common/images/thumbnails/14077d20717.jpgNuclear magnetic resonance (NMR) spectroscopy can be used to obtain a metabolic fingerprint of cancer of the bowel and so determine how far advanced the disease is, according to UK researchers.
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Mutations in the Saccharomyces cerevisiae succinate dehydrogenase result in distinct metabolic phenotypes revealed through (1)H NMR-based metabolic footprinting.
Mutations in the Saccharomyces cerevisiae succinate dehydrogenase result in distinct metabolic phenotypes revealed through (1)H NMR-based metabolic footprinting.
Mutations in the Saccharomyces cerevisiae succinate dehydrogenase result in distinct metabolic phenotypes revealed through (1)H NMR-based metabolic footprinting.
J Proteome Res. 2010 Dec 3;9(12):6729-39
Authors: Szeto SS, Reinke SN, Sykes BD, Lemire BD
Metabolomics is a powerful method of examining the intricate connections between mutations, metabolism, and disease. Metabolic...
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05-25-2011 07:01 PM
Metabolic profiling of cadmium-induced effects in one pioneer intertidal halophyte Suaeda salsa by NMR-based metabolomics.
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Ecotoxicology. 2011 May 15;
Authors: Liu X, Yang C, Zhang L, Li L, Liu S, Yu J, You L, Zhou D, Xia C, Zhao J, Wu H
Cadmium is a non-essential element to living organisms and has become the severe contaminant in both seawater and sediment in the intertidal zones of the Bohai Sea. The halophyte, Suaeda salsa...
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05-17-2011 06:21 PM
1H NMR-based metabolic profiling reveals inherent biological variation in yeast and nematode model systems
1H NMR-based metabolic profiling reveals inherent biological variation in yeast and nematode model systems
Abstract The application of metabolomics to human and animal model systems is poised to provide great insight into our understanding of disease etiology and the metabolic changes that are associated with these conditions. However, metabolomic studies have also revealed that there is significant, inherent biological variation in human samples and even in samples from animal model systems where the animals are housed under carefully controlled conditions. This inherent biological...
Metabolic profiling in kidneys of Atlantic salmon infected with Aeromonas salmonicida based on (1)H NMR.
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